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RESEARCH FOR UTILIZATION IN PLANT BREEDING AND ALTERRATION OF CYTOPLASM BY CELL FUSION.

Research Project

Project/Area Number 04806001
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Breeding science
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

MORI Koh-ichi  FACULTY OF AGRICULTURE,HOKKAIDO UNIVERSITY Associate Prof, 農学部, 助教授 (70166354)

Project Period (FY) 1992 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1994: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1993: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1992: ¥900,000 (Direct Cost: ¥900,000)
KeywordsCybrid / protoplast / Mitochondria / Asynmetric fusion / Cell fusion / Somatic hybrid / Rice / サイブリッド
Research Abstract

Breeding by cell fusion is excelent method, especialy in breeding program among species and/or genus. It's not neccesary to consider for commpatibility among them.
In this research, it tried to transfer cytoplasms related male sterile in rice plant (Oryza sativa L.), and to use cybrids gotten in this process were used for analysis of expression mechanism of cytoplasmic male sterile. Cell fusion were done by assinmetric, and fusion combination was between fertile and sterile.
1. Cybrids showing cytoplasmic male sterile were examined their mitochondrial genome by mtDNA cosmid clone covering all sequence. Two dmains were closely related for seed fertility of cybrid. One domain is including atp6 gene, and other's is located rps1 gene. This is the new information for gene related cytoplasmic male strile.
2. For variable characters of wild rice having cybrids, survey of blast desease does not show higher resistance than O.sativa.
3. Some sterile cybrids were grown fertile and sterile tillers through stock storage, and for these differences between fertility, the analysis of mitochondrial genome for these differences were coresponding to above two domains.

Report

(4 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • 1992 Annual Research Report

URL: 

Published: 1992-04-01   Modified: 2016-04-21  

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