Project/Area Number |
06670850
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Research Category |
Grant-in-Aid for General Scientific Research (C)
|
Allocation Type | Single-year Grants |
Research Field |
Dermatology
|
Research Institution | Asahikawa Medical College |
Principal Investigator |
SAKAI Hiroyuki (1995) Asahikawa Medical College Department of Dermatology Assistant, 医学部・皮膚科, 助手 (50205712)
松尾 忍 (1994) 旭川医科大学, 医学部, 助教授 (60125408)
|
Co-Investigator(Kenkyū-buntansha) |
坂井 博之 旭川医科大学, 医学部, 助手 (50205712)
|
Project Period (FY) |
1994 – 1995
|
Project Status |
Completed (Fiscal Year 1995)
|
Budget Amount *help |
¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1995: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1994: ¥600,000 (Direct Cost: ¥600,000)
|
Keywords | psoriasis / loricrin / involucrin / cornified cell enrelope / protein kinase C / 単クローン抗体 / ハイブリドーマ |
Research Abstract |
We analyzed CE formation in normal skin and in lesional psoriatic skin by immunoelectron microscopy. In the superficial granular cells of normal epidermis, involucrin labeling was detected along the plasma membrane, whereas loricrin staining was mostly distributed diffusely within the cells, occasionally with some additional granular aggregates within the cytoplasm and nucleus. Loricrin was also present predominantly on the desmosomal attachment plaques, colocalizing with desmoglein. In the cornified cells, involucrin labeling was reduced, whereas loricrin labeling was retained and continuously decorated the CEs, with relative sparing of the desmosomal areas. In typical psoriatic epidermis, involucrin staining was very intense but loricrin labeling was markedly reduced. The involucrin-positive CEs were formed precociously and further maturation of CE did not occur.
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