Fluctuations of hippocampal amini acids during the sleep-waking cycle
Project/Area Number |
07610087
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
実験系心理学
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Research Institution | Fukuoka Prefectural University |
Principal Investigator |
KAMETANI Hideki Fukuoka Prefectural Univ., Dept.Integ.Human Studies & Soc.Sci, Professor, 人間社会学部, 教授 (20167349)
|
Project Period (FY) |
1995 – 1997
|
Project Status |
Completed (Fiscal Year 1997)
|
Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 1996: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1995: ¥1,700,000 (Direct Cost: ¥1,700,000)
|
Keywords | RAT / HIPPOCAMPUS / AMINO ACID / ACETYLCHOLINE / SEROTININ / SLEEP-WAKING CYCLE / MICRODIALYSIS / HPLC / 一酸化窒素(NO) / NMDA / PBN / 老化 / グルタミン酸 / 睡眠-覚醒リズム / 一酸化窒素 / ポリグラフ / GABA |
Research Abstract |
In the present study, ACh release from forebrain regions including the dorsal hippocampus, the frontal cortex, or the striatum were continuously monitored in freely moving rats during the sleep-waking cycle in the light and dark periods using an in vivo microdialysis technique coupled to polygraph recordings. In the first experiment, the results clearly demonstrate that ACh release from hippocampus, cortex and striatum are subject to profound state-dependent fluctuations during stages of sleep-wakefulness during the light period. ACh output during active waking (AW) is higher than that during slow wave sleep (SWS) while ACh levels during quiet waking (QW) were higher than those during SWS,but significantly lower than AW.Further, the results indicate that ACh output during paradoxical sleep (PS) is as high as that during AW.Serotonin output in the hippocampus and the frontal cortax was highest during waking and lowest during PS.The level of serotonin during SWS was between those in waking and paradoxical sleep. In contrast, highest dopamine output was observed in the striatum during waking whereas those during SWS and PS did not differ each other. Hippocampal amino acid levels including glutamate, taurine and glycine were examined during waking, PS and SWS.After the penetration of the microdialysis probe into the hippocampus, glutamate level increased whereas levels of taurine and glycine decreased up to 8-10 hours. Once amino acid levels were stabilized, these levels stayd constant and did not show any fluctuation during the sleep-waking cycle.
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Report
(4 results)
Research Products
(11 results)