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脳血管攣縮の平滑筋細胞障害におけるアポトーシスの関与とサイトカインの効果の研究

Research Project

Project/Area Number 07671501
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cerebral neurosurgery
Research InstitutionDokkyo University School of Medicine (1996)
The University of Tokyo (1995)

Principal Investigator

KIM Phyo  Dokkyo University School of Medicine, Associate Professor, 医学部, 助教授 (90231290)

Co-Investigator(Kenkyū-buntansha) ASAI Akio  University of Tokyo Hospital, Lecture, 医学部, 講師 (50231858)
桐野 高明  東京大学, 医学部(病), 教授 (90126045)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1996: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1995: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsIntracellular calcium / Basilar artery / Dog / Cerebral arterial spasm / Viability / Smooth muscle / Subarachnoid hemorrhage / Fura-2
Research Abstract

The intracellular calcium level was determined in the canine basilar artery in order to investigate whether Ca2+ regulation of its smooth muscle is altered during chronic vasospasm following subarachnoid hemorrhage. A double hemorrhage model was used. The occurrence of vasospasm was confirmed angiographically seven days after initial hemorrhage. The intracellular calcium concentration ( [Ca2+] i) of smooth muscle was measured using Fura-2. Fluorescence to excitation at 340 and 356 nm was monitored and the ratio R340/356 was used as the indicator of [Ca2+] i. When the extracellular calcium concentration ( [Ca2+] e) was increased from pCa 8 to 2, [Ca2+] i also increased. In the spastic arteries, the [Ca2+] e- [Ca2+] i curve was elevated as compared to the normal rteries. Treatment with ionomycin elevated the curve in the normal group, but it had little effect in the spastic arteries. Values of [Ca2+] i, calculated in multiples of Kd, were greater in the spastic arteries. Diltiazem (10-5 mol/l) partially suppressed the augmented [Ca2+] i signal in the spastic arteries, whereas it did not affect the curve in the control group. These results indicate that calcium regulation of smooth muscle impaired after subarachnoid hemorrhage, which may contribute to the pathogenesis of chronic vasospasm.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] P.Kim,Y.Yoshimoto,M.Iino et al: "Impaind Calcium Regulation of Smooth Muscle During Chronic Vasospasm Following Subarachnoid Hemorrhage" J.Cerebral Blood Flow and Metabolism. 16. 334-341 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoshimoto Y.,Kim P.,Sasaki T.,et al: "Functional changes in cultured strips of canine cerebral arteries after prolonged exposure to oxyhemoglobin." J Neurosurg. 83. 867-874 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nagata K,Sasaki T,Mori T,Nikaido H,Kobayashi E,Kim P: "Cisternal talc injection in dog can induce delayed and prolonged constriction resembling cerebral vasospasm" Surg Neurol. 45. 442-447 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kim P:"Experimental models for subarachnoid hemorrhage and vasospasm.In:Piepgras DG and Yanagihara T(eds):Subarachnoid Hemorrhage." Marcel Dekker,New York,(1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kim P: "Pharmacological modification of experimental vasospasm.In:Piepgras DG and Yanagihara T(eds):Subarachnoid Hemorrhage." Marcel Dekker,New York, (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kim P,Yoshimoto Y,Iino M,Sasaki T,Kirino T,Nonomura Y.: "Increased Calcium Permeability of Sarcolemma during Chronic Vasospasm following Subarachnoid Hemorrhage." J Cereb Blood Flow Met. 16. 334-341 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoshimoto Y., Kim P., Sasaki T., et al: "Functional changes in cultured strips of canine cerebral arteries after prolonged exposure to oxyhemoglobin." J Neurosurg. 83. 867-874 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nagata K,Sasaki T,Mori T,Nikado H,Kobayashi E,Kim P,Kirino T: "Cisternal talc injection in dog can induce delayd and prolonged arterial constriction resembling cerebral vasospasm morphologically and pharmacologically." Surg Neurol. 45. 442-447 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kim P: Experimental models for subarachnoid hemorrhage and vasospasm. In : Piepgras DG and Yanagihara T (eds) : Subarachnoid Hemorrhage.Marcel Dekker, New York, (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kim P: Pharmacological modification of experimental vasospasm. In : Piepgras DG and Yanagihara T (eds) : Subarachnoid Hemorrhage.Marcel Dekker, New York (in press), (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] P.Kim,Y.Yoshimoto,M.Iino et al: "Impaind Calcium Regulation of Smooth Muscle During Chronic Vasospasm Following Subarachnoid Hemorrhage" J.Cerebral Blood Flow and Metabolism. 16. 334-341 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kim P: "Increased Calcium Bermeability of Sarcolemma durig Chronic Vasospasm" J Ceveb Blood Flow Met. in press. (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yoshimoto Y: "Functional Changes in Cultured Strips of Canine Cerebral Artenes" J.Neurosurg. 83. 867-874 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kim P(共同執筆): "Subarachnoid Hemorrhage" Marcel DeKKer(New YorK)(in press), (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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