• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1996 Fiscal Year Final Research Report Summary

脳血管攣縮の平滑筋細胞障害におけるアポトーシスの関与とサイトカインの効果の研究

Research Project

Project/Area Number 07671501
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cerebral neurosurgery
Research InstitutionDokkyo University School of Medicine (1996)
The University of Tokyo (1995)

Principal Investigator

KIM Phyo  Dokkyo University School of Medicine, Associate Professor, 医学部, 助教授 (90231290)

Co-Investigator(Kenkyū-buntansha) ASAI Akio  University of Tokyo Hospital, Lecture, 医学部, 講師 (50231858)
Project Period (FY) 1995 – 1996
KeywordsIntracellular calcium / Basilar artery / Dog / Cerebral arterial spasm / Viability / Smooth muscle / Subarachnoid hemorrhage / Fura-2
Research Abstract

The intracellular calcium level was determined in the canine basilar artery in order to investigate whether Ca2+ regulation of its smooth muscle is altered during chronic vasospasm following subarachnoid hemorrhage. A double hemorrhage model was used. The occurrence of vasospasm was confirmed angiographically seven days after initial hemorrhage. The intracellular calcium concentration ( [Ca2+] i) of smooth muscle was measured using Fura-2. Fluorescence to excitation at 340 and 356 nm was monitored and the ratio R340/356 was used as the indicator of [Ca2+] i. When the extracellular calcium concentration ( [Ca2+] e) was increased from pCa 8 to 2, [Ca2+] i also increased. In the spastic arteries, the [Ca2+] e- [Ca2+] i curve was elevated as compared to the normal rteries. Treatment with ionomycin elevated the curve in the normal group, but it had little effect in the spastic arteries. Values of [Ca2+] i, calculated in multiples of Kd, were greater in the spastic arteries. Diltiazem (10-5 mol/l) partially suppressed the augmented [Ca2+] i signal in the spastic arteries, whereas it did not affect the curve in the control group. These results indicate that calcium regulation of smooth muscle impaired after subarachnoid hemorrhage, which may contribute to the pathogenesis of chronic vasospasm.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] P.Kim,Y.Yoshimoto,M.Iino et al: "Impaind Calcium Regulation of Smooth Muscle During Chronic Vasospasm Following Subarachnoid Hemorrhage" J.Cerebral Blood Flow and Metabolism. 16. 334-341 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshimoto Y.,Kim P.,Sasaki T.,et al: "Functional changes in cultured strips of canine cerebral arteries after prolonged exposure to oxyhemoglobin." J Neurosurg. 83. 867-874 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nagata K,Sasaki T,Mori T,Nikaido H,Kobayashi E,Kim P: "Cisternal talc injection in dog can induce delayed and prolonged constriction resembling cerebral vasospasm" Surg Neurol. 45. 442-447 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kim P:"Experimental models for subarachnoid hemorrhage and vasospasm.In:Piepgras DG and Yanagihara T(eds):Subarachnoid Hemorrhage." Marcel Dekker,New York,(1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kim P: "Pharmacological modification of experimental vasospasm.In:Piepgras DG and Yanagihara T(eds):Subarachnoid Hemorrhage." Marcel Dekker,New York, (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kim P,Yoshimoto Y,Iino M,Sasaki T,Kirino T,Nonomura Y.: "Increased Calcium Permeability of Sarcolemma during Chronic Vasospasm following Subarachnoid Hemorrhage." J Cereb Blood Flow Met. 16. 334-341 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshimoto Y., Kim P., Sasaki T., et al: "Functional changes in cultured strips of canine cerebral arteries after prolonged exposure to oxyhemoglobin." J Neurosurg. 83. 867-874 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nagata K,Sasaki T,Mori T,Nikado H,Kobayashi E,Kim P,Kirino T: "Cisternal talc injection in dog can induce delayd and prolonged arterial constriction resembling cerebral vasospasm morphologically and pharmacologically." Surg Neurol. 45. 442-447 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kim P: Experimental models for subarachnoid hemorrhage and vasospasm. In : Piepgras DG and Yanagihara T (eds) : Subarachnoid Hemorrhage.Marcel Dekker, New York, (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kim P: Pharmacological modification of experimental vasospasm. In : Piepgras DG and Yanagihara T (eds) : Subarachnoid Hemorrhage.Marcel Dekker, New York (in press), (1997)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1999-03-09  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi