Budget Amount *help |
¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1996: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1995: ¥500,000 (Direct Cost: ¥500,000)
|
Research Abstract |
"Shosaikoto" in a dose of 100 mg/kg was orally administered for 4 days to 10 week old male Wistar rats and the experimental liver injury model was prepared by 1 hr liver ischemia 1 hr after last administration. After 1 hr blood reperfusion of the liver, GOT,GPT and LDH in the serum were estimated. Lipid peroxides in liver homogenates were measured and expressed as nmol of malonic dialdehyde formed per mg protein. Content of total glutathion, activities of cytochrome P-450, cytochrome b_5 and NADPH cytochrome Creductase were also estimated. The contents of lipid peroxide of control rats were 6.82 <plus-minus>0.54 and those of rats treated with "Shosaikoto" was decreased to 5.11<plus-minus>0.15. However, contents of total glutathion, activities of cytochrome P-450, cytochrome b_5 and NADPH cytochrome C reductase were not altered. "Shosaikoto" dose not seem to protect against liver injury. Following above experiments, "Daisaikoto" in doses of 100,200 and 400 mg/kg, or "Hochuekkito" in dos
… More
es of 200 and 400 mg/kg were orally administrered for 4 days to 8 week old male Wistar rats and the moderate experimental liver injury model was prepared by 15 min liver ischemia 1 hr after last administration. After 1 hr blood reperfusion of the liver, GOT,GPT and LDH in the serum were estimated. Lipid peroxides in liver homogenates were measured and expressed as n mol of malonic dialdehyde formed per mg protein. Contents of lipid peroxides of control rats (ischemia) was 7.06 <plus-minus>0.67 and those of rats treated with 200 mg/kg of "Daisaikoto" were 6.58<plus-minus>0.33 and 6.33 <plus-minus>0.55, respectively. Therefore, "Daisaikoto" tended to decrease the contents. However, the contents of rats treated with 200 mg/kg and 400 mg/kg of "Hochuekkito" were not decreased. Slight remission of liver injury was observed by treatment with "Daisaikoto" and "Hochuekito". It is concluded that remission of liver injury by "Shosaikoto" and "Daisaikoto" may occur by trapping lipid peroxides. That by "Hochuekito" may occur by different mechanism. Less
|