| Project/Area Number |
08670034
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | KEIO UNIVERSITY |
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Principal Investigator |
AISO Sadakazu KEIO UNIVERSITY,SCHOOL OF MEDICINE,PROFESSOR, 医学部, 教授 (60138013)
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| Co-Investigator(Kenkyū-buntansha) |
OGAWA Motoyuki KEIO UNIVERSITY,SCHOOL OF MEDICINE,ASSISTANT, 医学部, 助手 (90255422)
HIRAOKA Yoshiki KEIO UNIVERSITY,SCHOOL OF MEDICINE LECTURER, 医学部, 講師 (80218768)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1996: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | PROLACTION / GENE EXPRESSION / IMMUNOHISTOCHEMISTRY / IN SITU PCR / PROLACTIN RECEPTOR / in situ RT / PCR / 組織化学 / 脳室 / ホルモン |
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| Research Abstract |
WE have found that the prolactin (PRL) gene is expressed in the interstitial cells of the mouse kidney. The renal interstitial cells are categorized into several types. The fibroblast-like type-1 interstitial cells are assumed to produce mPRL.We prepared anti-mPRL receptor antibody to investigate immunohistochemically the localization of mPRL receptor protein in the mouse kidney. The receptor molecules are present in the brush border of renal tubules and plasma membrane of the interstitial cells. We presumed that the receptor located on the brush border might be involved in reabsorption of pituitary-derived blood-borne mPRL in the renal tubules. On the other hand, the mPRL receptor on the plasma membrane of the interstitial cell is perhaps the target of mPRL produced by the interstitial cells themselves. The renal mPRL acts in an autocrine/paracrine fashion in the interstitium. It is well known that a transcription factor, Pit-1, plays an essential role in transcription of the PRL gene in the pituitary. We suspected that Pit-1 would participate in mPRL transcription in mouse kidney. Our results demonstrated the presence of Pit-1 mRNA in the interstitial cells of the mouse kidney. This raises the possibility that Pit-1 also regulates the expression of the mPRL gene in the kidney.
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