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Expression and Abnormalities of Keratinocyte Terminal Differentiation-Associated Proteins

Research Project

Project/Area Number 08670940
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Dermatology
Research InstitutionASAHIKAWA MEDICAL COLLEGE

Principal Investigator

YAMAMOTO Akemi  Department of Dermatology, ASAHIKAWA MEDICAL COLLEGE Assistant Professor, 医学部, 講師 (30241441)

Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1996: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordsloricrin / cornified cell envelope / Vohwinkel's syndrome / progressive erythrokeratoderma / transglutaminase / keratinocytes / SPRR / involucrin / Vohwinkel症候群 / 角化 / 角化異常症
Research Abstract

Immunoelectron microscopic analysis of the process of cornified cell envelope formation in normal epidermis showed that involucrin is cross-linked earlier than loricrin (J Histochem Cytochem, 44 : 167-175,1996). Furthermore, we showed that cornified cell envelopes in psoriatic epidermis without granular layr was rich in involucrin and formed in a premature stage, while psoriatic epidermis with granular layr had envelopes similar to normal (J Histochem Cytochem, 44 : 167-175,1996). We also showed that psoriatic epidermis can be classified into two types ; epidermis with granular layr and without granular layr and these two have different proliferative situation when assessed by morphometry (Br J Dermatol 135 : 433-438,1996. J Invest Dermatol, 109 : 806-810,1997).
We induced expression of involucrin and SPRR in cultured normal human keratinocytes by increasing calcium concentration in the culture medium, and analyzed their localization and ultrastructural changes and showed that involucri … More n and SPRR were expressed and incorporated into the cornified cell envelopes in this order (J Invest Dermatol, 108 : 12-16,1997).
When we assessed expression patterns of keratinocytes differentiation-related proteins, we found that profilaggrin and trichohyalin had distinct expression patterns in normal and psoriatic epidermis, tongue epithelia and cultured keratinocytes despite that they have similar gene structures (Br J Dermatol, 137 : 9-16,1997). We further revealed that involucrin gene expression is stimulated by alpha and eta isoforms of protein kinase C (J Invest Dermatol, in press).
We showed that genetic keratinization disorders, Vohwinkel syndrome and progressive symmetric erythrokeratoderma were diseases of loricrin by morphological and molecular genetic studies (Nature Genetics, 13 : 70-77,1996. J Invest Dermatol, 109 : 604-610,1997, Am J Hum Genet, 61 : 581-589,1997) and we proposed that these diseases can be called loricrin keratoderma (Histol & Histopath, in press. Exp Dermatol, in press).
In the analysis of cornified cell envelope formation, disribution of envelope precursor protein, and morphology and function of epidermis in knockout mice for the transglutaminase 1 gene, it was found that cornified cell envelope formation, and distribution and cross-linking of loricrin and profilaggrin were disrupted, barrier function of the epidermis was severely decreased and mice died soon after birth (PNAS,in press). Less

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report

Research Products

(53 results)

All Other

All Publications

  • [Publications] A.Ishida-Yamamoto: "Immunoelectron microscopic analysis of cornified cell envelope formation in normal and psoriatic epidermis." J Histochem Cytochem. 44. 167-175 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] E.Maestrini: "A molecular defect in loricrin,the major component of the cornified cell envelope, underlies Vohwinkel's syndrome." Nature Genetics. 13. 70-77 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Iizuka: "Epidermal remodelling in psoriasis." Br J Dermatol. 135. 433-438 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A.Ishida-Yamamoto: "Eccrin syringofibroadenoma (Mascaro). An ultrastructural and immunohistochemical study." Am J Dematopathol. 18. 207-211 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A.Ishida-Yamamoto: "Involucrin and SPRR are synthesized sequentially in differentiating cultured epidermal cells." J Invest Dermatol. 108. 12-16 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Tamai: "Compound heterozygosity for a nonsense mutation and a splice site mutation in the type VII collagen gene (COL7A1) in recessive dystrophic epidermolysis bullosa." Laboratory Investigation. 76. 209-217 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A, Ishida-Yamamoto: "The molecular pathology of progressive symmetric erythrokeratodema:a frameshift mutation in the loricrin gene and pertubations in the cornified cell envelope." Am J Hum Genet. 61. 581-589 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A.Ishida-Yamamoto: "Distinctive expression of filaggrin and trichohyalin during various pathways of epithelial differentiation" Br J Dermatol. 137. 9-19 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] B.P.Korge: "Loricrin mutation in Vohwinkel's keratoderma is unique to the variant with ichthyosis" J Invest Dermatol. 109. 604-610 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Iizuka: "Another support for the location of epidermalstem cells residing adjacent to the tips of dermal papillae in the interfollicular epidermis" J Invest Dermatol. 109. 697 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Iizuka: "Epidermal Remodeling in psoriasis(II)" J Invest Dermatol. 109. 806-810 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A.Ishida-Yamamoto: "Loricrin and Human skin diseases : molecular basis of loricrin keratodermas." Histology and Histopathology. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A.Ishida-Yamamoto: "Structural organization of cornified cell envelopes and alterations in inherited skin disorders." Exp Dermatol. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Matsuki: "Defective stratum corneum and early neonatal death in mice lacking the gene for transglutaminase 1 (keratinocyte transglutaminase)." Proc Natl Acad Sci USA. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Takahashi: "The α and η isoforms of pretein kinase C stimulate transcription of human involucrin gene." J Invest Dermatol. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Tamura: "Functional alteration of guanine nucleotide binding proteins (Gs and Gi) in psoriatic epidermis." J Dermatol Sci. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Tanaka: "A Novel Variant of Acquired Epidermolysis Bullosa with Autoantibodies against the Central Triple-Helical Domain of Type VII Collagen." Laboratory Investigation. 77. 623-632 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A.Ishida-Yamamoto et al.: "Immunoelectron microscopic analysis of cornified cell envelope formation in normal and psoriatic epidermis." J Histochem Cytochem. 44. 167-175 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] E.Maestrini et al.: "A molecular defect in loricrin, the major component of the cornified cell envelope, underlies Vohwinkel's syndrome." Nature Genetics. 13. 70-77 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Iizuka, et al.: "Epidermal remodelling in psoriasis." Br J Dermatol. 135. 433-438 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A.Ishida-Yamamoto, et al.: "Eccrin syringofibroadenoma (Mascaro). An ultrastructural and immunohistochemical study." Am J Dermatopathol. 18. 207-211 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A.Ishida-Yamamoto, et al.: "Involucrin and SPRR are synthesized sequentially in differentiating cultured epidermal cells." J Invest Dermatol. 108. 12-16 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Tamai, et al.: "Compound heterozygosity for a nonsense mutation and a splice site mutation in the type VII collagen gene (COL7A1) in recessive dystrophic epidermolysis bullosa." Laboratory Investigation. 76. 209-217 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A Ishida-Yamamoto, et al.: "The molecular pathology of progressive symmetric erythrokeratoderma" Am J Hum Genet. 61. 581-589 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A Ishida-Yamamoto, et al.: "Distinctive expression of filaggrin and trichohyalin during various pathways of epithelial differentiation" Br J Dermatol. 137. 9-16 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] BP Korge, et al.: "Loricrin mutation in Vohwinkel's keratoderma is unique to the variant with ichthyosis" J Invest Dermatol. 109. 604-610 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H Iizuka, et al.: "Another support for the location of epidermal stem cells residing adjacent to the tips of dermal papillae in the interfollicular epidermis" J Invest Dermatol. 109. 697 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H Iizuka, et al.: "Epidermal Remodeling in psoriasis (II)" J Invest Dermatol. 109. 806-810 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A Ishida-Yamamoto, et al.: "Loricrin and Human skin diseases : molecular basis of loricrin keratodermas" Histology and Histopathology. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A Ishida-Yamamoto, et al.: "Structural organization of cornified cell envelopes and alterations in inherited skin disorders" Exp Dermatol. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M Matsuki, et al.: "Defective stratum corneum and early neonatal death in mice lacking the gene for transglutaminase 1 (keratinocyte transglutaminase)" Proc Natl Acad Sci. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H Takahashi, et al.: "The alpha and eta isoforms of protein kinase C stimulate transcription of human involucrin gene" J Invest Dermatol. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T Tamura, et al.: "Functional alteration of guanine nucleotide binding proteins (Gs and Gi) in psoriatic epidermis." J Dermatol Sci. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H Tanaka, et al.: "A Novel Variant of Acquired Epidermolysis Bullosa with Autoantibodies against the Central Triple-Helical Domain of Type VII Collagen." Lab Invest. 77. 623-632 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A.Ishida-Yamamoto: "Immunoelectron microscopic analysis of cornified cell envelope formation in normal and psoriatic epidermis." J Histochem Cytochem. 44. 167-175 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] E.Maestrini: "A molecular defect in loricrin,the major component of the cornified cell envelope,underlies Vohwinkel′s syndrome." Nature Genetics. 13. 70-77 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] H.Iizuka: "Epidermal remodelling in psoriasis." Br J Dermatol. 135. 433-438 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] A.Ishida-Yamamoto: "Eccrin syringofibroadenoma(Mascaro).An ultrastructural and immunohistochemical study." Am J Dermatopathol. 18. 207-211 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] A.Ishida-Yamamoto: "Involucrin and SPRR are synthesized sequentially in differentiating cultured epidermal cells." J Invest Dermatol. 108. 12-16 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] K.Tamai: "Compound heterozygosity for a nonsense mutation and a splice site mutation in the type VII collagen gene(COL7A1)in recessive dystrophic epidermolysis bullosa." Laboratory Investigation. 76. 209-217 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] A,Ishida-Yamamoto: "The molecular pathology of progressive symmetric erythrokeratoderma:a frameshift mutation in the loricrin gene and pertubations in the cornified cell envelope." Am J Hum Genet. 61. 581-589 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] A.Isida-Yamamoto: "Distinctive expression of filaggrin and trichohyalin during various pathways of epithelial differentiation" Br J Dermatol. 137. 9-19 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] B.P.Korge: "Loricrin mutation in Vohwinkel′s keratoderma is unique to the variant with ichthyosis" J Invest Dermatol. 109. 604-610 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] H.Iizuka: "Another support for the location of epidermal stem cells residing adjacent to the tips of dermal papillae in the interfollicular epidermis" J Invest Dermatol. 109. 697 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] H.Iizuka: "Epidermal Remodeling in psoriasis(II)" J Invest Dermatol. 109. 806-810 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] A.Ishida-Yamamoto: "Loricrin and Human skin diseases:molecular basis of loricrin keratodermas." Histology and Histopathology. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] A.Ishida-Yamamoto: "Structural organization of cornified cell envelopes and alterations in inherited skin disorders." Exp Dermatol. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Matsuki: "Defective stratum corneum and early neonatal death in mice lacking the gene for transglutaminase 1( keratinocyte transglutaminase)." Proc Natl Acad Sci USA. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] H.Takahashi: "The α and η isoforms of protein kinase C stimulate transcription of human involucrin gene." J Invest Dermatol. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Tamura: "Functional alteration of guanine nucleotide binding proteins(Gs and Gi) in psoriatic epidermis." J Dermatol Sci. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] H.Tanaka: "A Novel Variant of Acquired Epidermolysis Bullosa with Autoantibodies against the Central Triple-Helical Domain of Type VII Collagen." Laboratory Investigation. 77. 623-632 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Akemi Ishida-Yamamoto: "Involucrin and SPRR are synthesized sequentially in differentiating cultured epidermal cells" Journol of Investigative Dermatology. 108. 12-16 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] E.Maestrini: "A molecular defect in loricrin,the major component of the comified cell envelope,underlies Vohwinkel′s syndrome" Nature Genetics. 13. 70-77 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-03-31   Modified: 2016-04-21  

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