Project/Area Number |
08671626
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Cerebral neurosurgery
|
Research Institution | TAZUKE KOFOKAI MEDICAL RESEARCH INSTITUTE |
Principal Investigator |
NISHIOKA Tatsuya Tazuke Kofukai Medical Risearch Institute IV of Onocology, 医学研究所・第4研究部, 主任研究員 (10270563)
|
Project Period (FY) |
1996 – 1997
|
Project Status |
Completed (Fiscal Year 1997)
|
Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1997: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1996: ¥1,300,000 (Direct Cost: ¥1,300,000)
|
Keywords | human brain tomor / qlucose transporter / qlioblastoma / 糖代謝 / 画像診断 / 遺伝子療法 |
Research Abstract |
Purpose : Glucose is transported into cells via glucose transporter (GLUT). GLUT is subclassified into five, among which GLUT1 and GLUT3 are overexpressed in human glioma cells. The purpose of this study is to grope for new diagnostic methodes or therapy using GLUT.Results : 1) After sugar deprivation for three hours, mRNA of GLUT1 was found over expressed in all human tumor cell lines, whereas mRNA of GLUT3 was found overexpressed in 7/9 human glioma cell lines and was not expressed in human neuronal tumor cell lines. 2) In five glioma cell lines, expression of both GLUT1 and GLUT3 was found on protein level. 3) In vitro, T98G cells was more inhibited by GLUT3 polyclonal antibody as its concentration increased. 4) Using autoradiography, 32P-labelled antisense of GLUT3 was relatively specifically detected in brain tumor model of T98G-microinjected nude mice.
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