Study on substances regulating pollen germination in Japanese chestnut
Project/Area Number |
09660038
|
Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
園芸・造園学
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Research Institution | Miyagi Agricultural College |
Principal Investigator |
NAKAMURA Masahiro Miyagi Agricultural College, Horticulture, Professor, 園芸科, 教授 (30149091)
|
Project Period (FY) |
1997 – 1999
|
Project Status |
Completed (Fiscal Year 1999)
|
Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1999: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 1998: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1997: ¥1,800,000 (Direct Cost: ¥1,800,000)
|
Keywords | repressive substances of pollen germination / promotive substances of pollen germination / solution extracted with water from styles / pollen germination on stigma / development of ovules / substances regulating pollen germination / shikimic acid / sugar / 花柱抽出液 / 花粉発芽 / 花柱伸長初期 / 花柱伸長終了期 / 胚珠形成 / クリ開花と花粉発芽 / 花粉発芽・花粉管伸長抑制 / 花粉管 / フェノール物質 / ポリフェノール / ケイヒ酸 |
Research Abstract |
The study was conducted to clarify the substances regulating pollen germination and pollen tube growth in the styles of Japanese chestnut (Castanea crenata Sieb. Et Zucc.) in concerned with development of ovules. Pollen germination and pollen tube growth on the stigma began after the style ceased to grow, although the anthesis of male flowers had already began. The solution extracted with water from the styles at early stage of the growth repressed remarkably pollen germination and pollen tube growth, although its repressive effect disappeared as the style ceased the growth. The HPLC analysis of the extracted solution showed that repressive substance might be shikimic acid, as estimated from retention time. Sugar content in the extracted solution was higher both at early stage of the style growth and at the beginning of pollen germination and pollen tube growth on the stigma. When pollen tubes began to grow on the stigma, the development of ovules was at early stage in which no nucellus was formed in each ovules in a ovary. The HPLC analysis of the extracted solution under different analytical condition showed the possibility of existence of both repressive and promotive substances which were produced by styles before and after pollen germination and pollen tube growth began on the stigma, respectively. However, no distinct relationship between the production of those substances and the development of ovules was indicated. Therefore, it was concluded that pollen germination and pollen tube growth on the stigma were regulated by the substances produced from stigma and style themselves.
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Report
(4 results)
Research Products
(7 results)