|Budget Amount *help
¥1,300,000 (Direct Cost : ¥1,300,000)
Fiscal Year 1999 : ¥500,000 (Direct Cost : ¥500,000)
Fiscal Year 1998 : ¥800,000 (Direct Cost : ¥800,000)
Chara internodal cells can be killed in aqueous electrolyte solutions such as KCl, NaCl, MgCl_2 and BaCl_2. During this process, Ca^<2+> is released from Chara cell walls. Including this phenomenon, by a grant-in-aid sponsored from 1998 till 1999, the following subjects were clarified. Namely, (1) although a main chemical compound of calcium bands on Chara cell walls has been reported to be CaCO_3, is it true? (2) It is the electroneutrality rule that is essential for governing ion transport processes in the cell membrane. How can be the electroneutrality rule theoretically verified? (3) Whether or not electroneutrality is contradictory to electrogenicity. (4) Can active transport systems transport so much amount of a certain specific ion across the cell membrane independently from other ones that the concentration can be detected by usual instruments such as a pH meter, ion chromatograph, atomic absorption apparatus? In other words, whether or not electroneutrality does not necessarily hold true in active ion transport systems. In relation to (1), CaHPO_4 has been identified to coexist in calcium bands. In relation to (2) and (3), the electroneutrality rule has been verified based on a novel another standpoint than those so far and it has been verified that electroneutrality does not practically contradict to electrogenicity. In relation to (4), it has been verified that any active ion transport system cannot transport a specific ion so much independently from other ions that its concentration can be changed largely.