| Project/Area Number |
12671303
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | Fukui Medical University |
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Principal Investigator |
CHIBA Yukio Fukui Medical University 2nd department of surgery, assistant professor, 医学部, 助教授 (30111959)
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| Co-Investigator(Kenkyū-buntansha) |
KIMURA Tetsuya Fukui Medical University 2nd department of surgery, assistant, 医学部, 助手 (50195369)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2001: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2000: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | PD-ECGFAT / angiogenesis / schemic myocardium / apptosis / 遺伝子治療 / apoptosis / pCI vector / platelet-derived endothelial cell growth factor / 虚血性心疾患 |
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| Research Abstract |
Angiogenesis is a promising novel therapeutic strategy to provide new venues for blood flow in patients with severe ischemic heart arid peripheral vascular disease, who are not candidates for standard revascularization strategies.
Platelet-derived endothelial cell gro\yth factor (PD-ECGF) is also identical to thymidine phosphorylase (TP), catalyze the reversible phosphorolysis of thymidine to thymine and 2'-deoxy-D-ribose-1-phosphate. PD-ECGFfTP has been shown to possess angiogenic activity in and confers resistance to apoptosis induced by hypoxia.
This study examine the effectiveness of delivery a mammalian expression plasmid vector encoding human full length PD-ECGF/TP complementary DNA in the chronic canine ischemic myocardium. We found that injecting of PD-ECGF/TP gene not only induced angiogenesis, myocardiac function improvement, but also inhibited the apoptosis of the myocardial cells. The platelet-derived endothelial cell growth factor may be a influential candidate gene for gene therapeutic angibgenesis.
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