2001 Fiscal Year Final Research Report Summary
Angiogenic therapy for Chronic Ischemic Myocardium with Platelet-derived Endothelial Cell Growth Factor Gene in aDog Model
Project/Area Number |
12671303
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Thoracic surgery
|
Research Institution | Fukui Medical University |
Principal Investigator |
CHIBA Yukio Fukui Medical University 2nd department of surgery, assistant professor, 医学部, 助教授 (30111959)
|
Co-Investigator(Kenkyū-buntansha) |
KIMURA Tetsuya Fukui Medical University 2nd department of surgery, assistant, 医学部, 助手 (50195369)
|
Project Period (FY) |
2000 – 2001
|
Keywords | PD-ECGFAT / angiogenesis / schemic myocardium / apptosis / 遺伝子治療 / apoptosis |
Research Abstract |
Angiogenesis is a promising novel therapeutic strategy to provide new venues for blood flow in patients with severe ischemic heart arid peripheral vascular disease, who are not candidates for standard revascularization strategies. Platelet-derived endothelial cell gro\yth factor (PD-ECGF) is also identical to thymidine phosphorylase (TP), catalyze the reversible phosphorolysis of thymidine to thymine and 2'-deoxy-D-ribose-1-phosphate. PD-ECGFfTP has been shown to possess angiogenic activity in and confers resistance to apoptosis induced by hypoxia. This study examine the effectiveness of delivery a mammalian expression plasmid vector encoding human full length PD-ECGF/TP complementary DNA in the chronic canine ischemic myocardium. We found that injecting of PD-ECGF/TP gene not only induced angiogenesis, myocardiac function improvement, but also inhibited the apoptosis of the myocardial cells. The platelet-derived endothelial cell growth factor may be a influential candidate gene for gene therapeutic angibgenesis.
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