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Identification and characterization of plant microtubule-associated proteins

Research Project

Project/Area Number 13440244
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物生理
Research InstitutionHimeji Institute of Technology

Principal Investigator

SONOBE Seiji  University of Hyogo, Graduate school of life science, Associate professor, 大学院・理学研究科, 助教授 (30197024)

Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥15,000,000 (Direct Cost: ¥15,000,000)
Fiscal Year 2003: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2002: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2001: ¥9,600,000 (Direct Cost: ¥9,600,000)
KeywordsPlant Cell / microtubule / microtubule-associated protein / cortical microtubule / morphogenesis / cell division / MAPs / 65kDa MAP / タバコ培養細胞BY-2 / ミニプロトプラスト / MAP210 / MAP65 / 細胞骨格 / タバコBY-2細胞
Research Abstract

A microtubule-associated protein, MAP200, was purified from miniprotoplasts of tobacco BY-2 cells and characterized. As a result; 1, the MAP200 is identical with the TMBP200 which was purified from tobacco BY-2 cells and characterized by Yasuhara et at.; 2, MAT200 did not bundle microtubules differently from the TMBP200; 3, the MAP200 accelrates tubulin polymerization; 4, The MAP200 forms a complex with tubulin; 5, the MAP200 predominantly exists in the cytoplasm and partly does on microtubules. These results lead us to have a hypothesis that a complex consted of the MAP200 and tubulin exists in the cytoplasm and accelrated microtubule elongation by shuttling mechanism. On the other hand. I and Dr.Hussey were collaborated and elucidated some propertied of MAP65; 1, Ala420 is important for its MT binding;2,pleiade4, a mutant in mitosis, has amino acid substitution for Ala420;3, the MAP65 forms a homodimer and the n-terminal region is important for this. In addition, we examined the participation of protein phosphorylation in bundling of cortical MTs and we found that DMAP disorganized cortical MTs in vivo and isolated cortical MTs were dispersed on treatment with phosphatase. These results strongly suggest that bundling of cortical MTs is regulated by phosphorylation of the MAP65.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Sonobe S, Yamamoto S, Motomura M, Shimmen T: "Isolation of Cortical MTs from Tobacco BY-2 Cells"Plant Cell Physiol. 42. 162-169 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yasuhara H, Muraoka M, Shogaki H, Sonobe S: "TMBP200, a Microtubule Bundling Polypeptide Isolated from Telophase Tobacco BY-2 Cells is a MOR1 Homologue"Plant Cell Physiol. 43. 595-603 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Inada S, Sonobe S, Shimmen T: "Regulation of directional expansion by the cortical microtubule array in roots of Lemna minor"Funct.Plant Biol. 29. 1273-1278 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fujiwara S, Matsuda N, Sato T, Sonobe S, Maeshima M: "Molecular Properties of a Matrix Attachment Region-Binding Protein Located in the Nucleoli of Tobacco Cells"Plant Cell Physiol. 43. 1558-1567 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamada S, Sonobe S, Shimmen T: "Synthesis of a Callosic Substance during Rhizoid Differentiation in Spirogyra"Plant Cell Physiol. 44. 1225-1228 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoshida K, Sonobe S, Shimmen T: "Involvement of microtubules in rhizoid differentiation of Spirogyra species"Protoplasma. 221. 227-235 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sonobe S, Yokota T, Shimmen T: "Tobacco BY-2 cells as an idea material for Biochemical Studies of plant cytoskeletic pattern"Springer. (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sonobe, S., Yamamoto, S., Motomura, M., Shimmen, T.: "Isolation of cortical MTs from tobacco By-2 cells"Plant Cell Physiol. 42. 162-169 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yasuhara, H., Muraoka M., Shogaki, H., Sonobe, S.: "TMBP200, a microtubule-bundling polypeptide isolated from telophase tobacco BY-2 cells is a MOR 1 homolog"Plant Cell Physiol. 43. 595-603 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Inada, S., Sonobe, S., Shimmen, T.: "Regulation of directional expansion by the cortical microtubule array in roots of Lemna Minor"Funct.Plant.Biol.. 29. 1273-1278 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fujiwara, S., Matsuda, N., Sato, T., Sonobe, S., Maeshima, M.: "Molecular properties of a matrix attachment region-binding protein located in the nucleoli of tobacco cells"Plant Cell Physiol. 43. 1558-1567 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamada, S., Sonobe, S., Shimmen, T.: "Synthesis of a callosic substance during rhizoid differentiation in Spirogyra"Plant Cell Physiol. 44. 1225-1228 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoshida, K., Sonobe, S., Shimmen, T.: "Involvement of microtubules in rhizoid differentiation of Spirogyra species"Protoplasma. 221. 227-235 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sonobe, S., Yokota, E., Shimmen, T.: "Tobacco BY-2 cells as an ideal material for biochemical studies of plant cytoskeletal proteins"Biotechnology in Agriculture and Forestry. 53. 98-112 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] S.SONOBE, E.YOKOTA, T.SHIMMEN: "Tobacco BY-2 cells as an ideal material for Biochemical studies of plant cytoskeletal proteins"Biotech.Apr.Forest. 53. 98-112 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] S.YAMADA, S.SONOBE, T.SHIMMEN: "Synthesis of a Callosic Substance during Rhizoid Differentiation in Spirogyra"Plant Cell Physiol. 44. 1225-1228 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] S.Fujiwara, N.Matsuda, T.Sato, S.Sonobe, M.Maeshima: "Molecular properties of a unique matrix attachment region-binding protein located in the nucleoli of tobacco cells."Plant Cell Physiol. 43. 1558-1567 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] K.Yoshida, S.Sonobe, T.Shimmen: "Involvement of microtubules in rhizoid differentiation of Spirogyra species"Protoplasma. 221. 227-235 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Inada, S., Sonobe, S., Shimmen T.: "Regulation of directional expansion by the cortical microtubule array in roots of Lemna minor"Funct.Plant Biol.. 29. 1273-1278 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] S.SONOBE, S.YAMAMOTO, M.MOTOMURA, T.SHIMMEN: "Isolation of Cortical MTs from Tobacco BY-2 Cells"Plant and Cell Physiology. 42. 162-169 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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