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2003 Fiscal Year Final Research Report Summary

Identification and characterization of plant microtubule-associated proteins

Research Project

Project/Area Number 13440244
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物生理
Research InstitutionHimeji Institute of Technology

Principal Investigator

SONOBE Seiji  University of Hyogo, Graduate school of life science, Associate professor, 大学院・理学研究科, 助教授 (30197024)

Project Period (FY) 2001 – 2003
KeywordsPlant Cell / microtubule / microtubule-associated protein / cortical microtubule / morphogenesis / cell division
Research Abstract

A microtubule-associated protein, MAP200, was purified from miniprotoplasts of tobacco BY-2 cells and characterized. As a result; 1, the MAP200 is identical with the TMBP200 which was purified from tobacco BY-2 cells and characterized by Yasuhara et at.; 2, MAT200 did not bundle microtubules differently from the TMBP200; 3, the MAP200 accelrates tubulin polymerization; 4, The MAP200 forms a complex with tubulin; 5, the MAP200 predominantly exists in the cytoplasm and partly does on microtubules. These results lead us to have a hypothesis that a complex consted of the MAP200 and tubulin exists in the cytoplasm and accelrated microtubule elongation by shuttling mechanism. On the other hand. I and Dr.Hussey were collaborated and elucidated some propertied of MAP65; 1, Ala420 is important for its MT binding;2,pleiade4, a mutant in mitosis, has amino acid substitution for Ala420;3, the MAP65 forms a homodimer and the n-terminal region is important for this. In addition, we examined the participation of protein phosphorylation in bundling of cortical MTs and we found that DMAP disorganized cortical MTs in vivo and isolated cortical MTs were dispersed on treatment with phosphatase. These results strongly suggest that bundling of cortical MTs is regulated by phosphorylation of the MAP65.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Sonobe S, Yamamoto S, Motomura M, Shimmen T: "Isolation of Cortical MTs from Tobacco BY-2 Cells"Plant Cell Physiol. 42. 162-169 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yasuhara H, Muraoka M, Shogaki H, Sonobe S: "TMBP200, a Microtubule Bundling Polypeptide Isolated from Telophase Tobacco BY-2 Cells is a MOR1 Homologue"Plant Cell Physiol. 43. 595-603 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Inada S, Sonobe S, Shimmen T: "Regulation of directional expansion by the cortical microtubule array in roots of Lemna minor"Funct.Plant Biol. 29. 1273-1278 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fujiwara S, Matsuda N, Sato T, Sonobe S, Maeshima M: "Molecular Properties of a Matrix Attachment Region-Binding Protein Located in the Nucleoli of Tobacco Cells"Plant Cell Physiol. 43. 1558-1567 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamada S, Sonobe S, Shimmen T: "Synthesis of a Callosic Substance during Rhizoid Differentiation in Spirogyra"Plant Cell Physiol. 44. 1225-1228 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshida K, Sonobe S, Shimmen T: "Involvement of microtubules in rhizoid differentiation of Spirogyra species"Protoplasma. 221. 227-235 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sonobe S, Yokota T, Shimmen T: "Tobacco BY-2 cells as an idea material for Biochemical Studies of plant cytoskeletic pattern"Springer. (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sonobe, S., Yamamoto, S., Motomura, M., Shimmen, T.: "Isolation of cortical MTs from tobacco By-2 cells"Plant Cell Physiol. 42. 162-169 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yasuhara, H., Muraoka M., Shogaki, H., Sonobe, S.: "TMBP200, a microtubule-bundling polypeptide isolated from telophase tobacco BY-2 cells is a MOR 1 homolog"Plant Cell Physiol. 43. 595-603 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Inada, S., Sonobe, S., Shimmen, T.: "Regulation of directional expansion by the cortical microtubule array in roots of Lemna Minor"Funct.Plant.Biol.. 29. 1273-1278 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fujiwara, S., Matsuda, N., Sato, T., Sonobe, S., Maeshima, M.: "Molecular properties of a matrix attachment region-binding protein located in the nucleoli of tobacco cells"Plant Cell Physiol. 43. 1558-1567 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamada, S., Sonobe, S., Shimmen, T.: "Synthesis of a callosic substance during rhizoid differentiation in Spirogyra"Plant Cell Physiol. 44. 1225-1228 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshida, K., Sonobe, S., Shimmen, T.: "Involvement of microtubules in rhizoid differentiation of Spirogyra species"Protoplasma. 221. 227-235 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sonobe, S., Yokota, E., Shimmen, T.: "Tobacco BY-2 cells as an ideal material for biochemical studies of plant cytoskeletal proteins"Biotechnology in Agriculture and Forestry. 53. 98-112 (2003)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2005-04-19  

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