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Molecular mechanism of scarless wound healing its application for therapy of epidermolysis bullosa

Research Project

Project/Area Number 13670861
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Dermatology
Research InstitutionHirosaki University

Principal Investigator

KON Atsushi  Hirosaki University, School of Medicine, Biochemistry, Associate Professor, 医学部, 助教授 (60271798)

Co-Investigator(Kenkyū-buntansha) MATSUZAKI Yasushi  Hirosaki University, University Hospital, Dermatology, Instructor, 医学部附属病院, 助手 (50322946)
TAKAGAKI Keiichi  Hirosaki University, School of Medicine, Biochemistry, Professor, 医学部, 教授 (70163160)
玉井 克人  弘前大学, 医学部, 助教授 (20236730)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 2003: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2002: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2001: ¥1,700,000 (Direct Cost: ¥1,700,000)
Keywordsscarless wound healing / scar / hyaluronic acid / regeneration / tissue engineering / SAGE
Research Abstract

In order to investigate the molecular mechanism of scarless wound healing and identify its specific gene/protein, that is, master gene/protein, we analyzed gene expression in the fetal mouse skin, which scarless wound healing is observed, by differential display and serial analysis gene expression techniques. Differential display analyses revealed that over ten kinds of scarless wound healing-related genes including genes previously reported such as fibromodulin, glycophorin, HOX and PRX families, TGF-beta, PDGH and EGF genes were detected. Furthermore several unknown genes were also detected. Serial analysis gene expression analyses detected over twenty kinds of genes including unknown genes However, introductions the expression vector of each gene or its recombinant protein into the wounds of adult mice skin did not disappear scar formation. Therefore, each gene identified in this study was not master gene of scarless wound healing. Hyaluronic acid is specifically accumulated in the dermis where scarless wound healing is observed, suggesting hyaluronic acid is one of crucial modulator of mechanism of scarless wound healing. Therefore, we next investigated hyaluronic acid rich environment specific genes. Serial analysis gene expression analyses of cultured human dermal fibroblasts containing high amounts of hyaluronic acid revealed similar results to these detected in scarless wound healing. Thirty five hyaluronic acid rich environment specific genes were detected. Seven of them were unknown. Howerver, introduction of these genes into wounded skin also did not disappear scar formation. These results suggest that all genes identified in this study are not master gene but specific gene of scarless wound healing. Therefore, further novel analysis methods and strategies are necessary to find unknown master gene.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Atsushi Kon: "Regulation of type VII collagen gene expression and its possible involvement in photoaged skin characterized by wrinkle formation."Fragrance Journal. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hideyuki Sasaki: "A novel Sp1-family mediated cis-acting element for transcription of type VII collagen gene (COL7A1)."J Dermatol Sci. 32. 239-242 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yasushi Matsuzaki: "Keratinocyte responsive element 3 : analysis of a keratinocyte- specific regulatory sequence in the 230 kDa bullus pemphigoid antigen gene promoter."J Invest Dermatol. 120. 308-312 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Atsushi Kon: "Novel transglutaminase 1 gene mutations (R348X/Y365D) in a Japanese family with lamellar ichthyosis."J Invest Dermatol. 120. 170-172 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Daisuke Sawamura: "Identification of COL7A1 alternative splicing inserting 9 amino acid residues into the fibronectin type III linker domain."J Invest Dermatol. 120. 942-948 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Atsushi Kon: "Regulation of type VII collagen gene expression in epidermal keratinocytes and dermal fibroblasts"Connec Tissue. 34. 127-135 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kon A: "Regulation of type VII collagen gene expression and its possible involvement in photoaged skin characterized by wrinkle formation."Fragrance J. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sasaki H, Kon A, Takeda H, Sawamura D, Kakizaki I, Takagaki K, Hashimoto I, Hanada K: "A novel Sp1-family mediated cis-acting element for transcription of type VII collagen gene(COL7A1)."J Dermatol Sci. 32. 239-242 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Matsuzaki Y, Tamai K, Kon A, Sawamura D, Uitto J, Hashimoto I: "Keratinocyte responsive element 3 : analysis of a keratinocyte-specific regulatory sequence in the 230kDa bullus pemphigoid antigen gene promoter."J Invest Dermatol. 120. 308-312 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kon A, Takeda H, Sasaki H, Yoneda K, Nomura K, Ahvazi B, Steinert PM, Hanada K, Hashimoto I: "Novel transglutaminase 1 gene mutations(R348X/Y365D) in a Japanese family with lamellar ichthyosis."J Invest Dermatol. 120. 170-172 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sawamura D, Goto M, Yasukawa K, Kon A, Akiyama M, Shimizu H: "Identification of COL7A1 altemative splicing inserting 9 amino acid residues into the fibronectin type III linker domain."J Invest Dermatol. 120. 942-948 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kon A: "Regulation of type VII collagen gene expression in epidermal keratinocytes and dermal fibroblasts."Connec Tissue. 34. 127-135 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary

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Published: 2001-04-01   Modified: 2016-04-21  

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