| Project/Area Number |
13672134
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Hyogo College of Medicine |
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Principal Investigator |
TOKUNAGA Atsushi Hyogo College of Medicine, Assistant Professor, 医学部, 講師 (70254521)
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| Co-Investigator(Kenkyū-buntansha) |
KONDO Eiji Matsumoto Dental University, Professor, 歯学部, 教授 (50273636)
NOGUCHI Koichi Hyogo College of Medicine, Professor, 医学部, 教授 (10212127)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2001: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | neuritis / peripheral nerve / cytokine / glia / dorsal root ganglion / nerve injury / rat / immunohistochemistry |
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| Research Abstract |
Eliav et al. (Pain 83 162-182, 1999) reported that peripheral neuritis of the rat sciatic nerve induced hyperalgesia and allodynia.
We modified the Eliav's model in order to prevent physical stimulation to the sciatic nerve. Oxycel was directly placed on the sciatic nerve without any touch of instruments, and saturated with Complete Freund's Adjuvant (CFA). ED-1 (a marker for macrophages), TNFalpha and IL-1beta immunoreactivity were detected from post-operative day 1 by immunohistochemistry. TNFalpha and IL-1beta immunoreactive cells were thought to be Schwann cells. Myelin basic protein was abolished on the sciatic nerve. This suggests that demuelination was occurred in the sciatic nerve. Furthermore, dorsal root ganglion neurons expressed Neuropeptide Y, GAP-43 and ATF3, which were well known to be expressed on axotomized DRG neurons.
Those findings suggested that activating of peripheral immune system might induce peripheral nerve injury.
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