Structural study of light-driven sodium pump
Project/Area Number |
15H02391
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Research Category |
Grant-in-Aid for Scientific Research (A)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Biophysics
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Research Institution | Nagoya Institute of Technology |
Principal Investigator |
Kandori Hideki 名古屋工業大学, 工学(系)研究科(研究院), 教授 (70202033)
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥40,430,000 (Direct Cost: ¥31,100,000、Indirect Cost: ¥9,330,000)
Fiscal Year 2017: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
Fiscal Year 2016: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
Fiscal Year 2015: ¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
|
Keywords | ロドプシン / ナトリウムポンプ / 光反応サイクル / 結晶構造解析 / 赤外分光 |
Outline of Final Research Achievements |
Although it was believed that rhodopsins cannot pump non-proton cations, we discovered a light-driven sodium pump rhodopsin in 2013. This project aims at revealing the structure of sodium pump rhodopsins. In collaboration with Nureki Lab of Univ. Tokyo, we successfully crystalized a light-driven sodium pump KR2, and proposed a model of sodium pumping. We also performed light-induced difference FTIR spectroscopy, such as low-temperature, time-resolved, and attenuated total reflection (ATR) methods, to sodium pump rhodopsins, and obtained important structural information. We reported more than 50 articles in Nature, Nature Commun., JACS, Angew., PNAS, JPC Letters and JBC during the project period.
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Report
(4 results)
Research Products
(355 results)
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[Journal Article] “Effect of point mutations on the ultrafast photo-isomerization of Anabaena sensory rhodopsin.”2018
Author(s)
D. Agathangelou, Y. Orozco-Gonzalez, M. del Carmen Marín, P. P. Roy, J. Brazard, H. Kandori, K. -H. Jung, J. Léonard, T. Buckup, N. Ferre;, M. Olivucci, S. Haacke*
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Journal Title
Faraday Discuss.
Volume: 207
Pages: 55-75
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] A natural light-driven inward proton pump2016
Author(s)
Keiichi Inoue, Shota Ito, Yoshitaka Kato, Yurika Nomura, Mikihiro Shibata, Takayuki Uchihashi, Satoshi P. Tsunoda and Hideki Kandori
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Journal Title
Nature Communications
Volume: 7
Issue: 1
Pages: 13415-13415
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] "Toward automatic rhodopsin modeling as a tool for high-throughput computational photobiology"2016
Author(s)
F. Melaccio, M. del Carmen Marin, A. Valentini, F. Montisci, S. Rinaldi, M. Cherubini, X. Yang, Y. Kato, M. Stenrup, Y. Orozco-Gonzalez, N. Ferre, H. L. Luk, H. Kandori, M. Olivucci*
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Journal Title
J.Chem. Theory Comput.
Volume: 12
Issue: 12
Pages: 6020-6034
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Functional Conversion of CPD and (6-4) Photolyases by Mutation2016
Author(s)
Daichi Yamada, Hisham M. Dokainish, Tatsuya Iwata, Junpei Yamamoto, Tomoko Ishikawa, Takeshi Todo, Shigenori Iwai, Elizabeth D. Getzoff, Akio Kitao, and Hideki Kandori
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Journal Title
Biochemistry
Volume: 55
Issue: 30
Pages: 4173-4183
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Structural basis for Na+ transport mechanism by a light-driven Na+ pump2015
Author(s)
H. E. Kato, K. Inoue, R. Abe-Yoshizumi, Y. Kato, H. Ono, M. Konno, S. Hososhima, T. Ishizuka, M. R. Hoque, H. Kunitomo, J. Ito, S. Yoshizawa, K. Yamashita, M. Takemoto, T. Nishizawa, R. Taniguchi, K. Kogure, A. D. Maturana, Y. Iino, H. Yawo, R. Ishitani, H. Kandori*, O. Nureki*
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Journal Title
Nature
Volume: 未定
Issue: 7550
Pages: 48-53
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] "Function conversion between CPD and (6-4) photolyases"2017
Author(s)
D. Yamada, H. M. Dokainish, T. Iwata, J. Yamamoto, T. Ishikawa, T. Todo, S. Iwai, E. D. Getzoff, K. Yura, A. Kitao, H. Kandori
Organizer
19th International Union of Pure and Applied Biophysics (IUPAB) and 11th European Biophysical Societies' Association (EBSA) Congress
Related Report
Int'l Joint Research
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[Presentation] "Functional conversion of CPD and (6-4) photolyases"2017
Author(s)
D. Yamada, H. M. Dokainish, T. Iwata, J. Yamamoto, T. Ishikawa, T. Todo, S. Iwai, E. D. Getzoff, K. Yura, A. Kitao, H. Kandori
Organizer
Soft molecular systems, 5th symposium
Related Report
Int'l Joint Research
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[Presentation] "Natural light-driven inward proton pump"2016
Author(s)
K. Inoue, S. Ito, Y. Kato, Y. Nomura, M. Shibata, T. Uchihashi, S. P. Tsunoda, H. Kandori
Organizer
The 54th Annual Meeting of the Biophysical Society of Japan
Place of Presentation
tsukuba
Year and Date
2016-11-25
Related Report
Int'l Joint Research
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[Presentation] 光で陽イオンを運ぶ2016
Author(s)
神取秀樹
Organizer
理化学研究所「分子システム研究」研究報告会
Place of Presentation
和光
Year and Date
2016-02-03
Related Report
Invited
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[Presentation] Functional conversion of CPD and (6-4) photolyases by mutation2015
Author(s)
D. Yamada, H. M. Dokainish, T. Iwata, J. Yamamoto, T. Ishikawa, T. Todo, S. Iwai, E. D. Getzoff, A. Kitao, H. Kandori
Organizer
The 53rd Annual Meeting of the Biophysical Society of Japan
Place of Presentation
Kanazawa, Japan
Year and Date
2015-09-13
Related Report
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[Presentation] Functional conversion between photolyases by mutation2015
Author(s)
D. Yamada, T. Iwata, J. Yamamoto, T. Ishikawa, T. Todo, S. Iwai, E. D. Getzoff, H. Kandori
Organizer
The 3rd Awaji International Workshop on "Electron Spin Science & Technology: Biological and Materials Science Oriented Applications"
Place of Presentation
Awaji Island, Japan
Year and Date
2015-06-14
Related Report
Int'l Joint Research
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