Regulatory mechanisms of replication origin activation.

• Project/Area Number: 15H04330
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Molecular biology
• Research Institution: Osaka University
• Principal Investigator: Masukata Hisao 大阪大学, 理学研究科, 教授 (00199689)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000); Fiscal Year 2017: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2016: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
• Keywords: テロメア / 複製開始 / 細胞周期 / 分裂酵母 / shelterin / Taz1 / Rif1 / PP1 / 複製開始点 / 染色体 / 核内局在 / 複製タイミング / DNA複製 / クロマチン / 細胞核 / Mcm10 / デグロン

Outline of Final Research Achievements

We found that the N-terminal 119 amino acid region of fission yeast Sld3 interacts each other and stabilizes Sld3 protein in the cells. Thus, it contributes to formation of bidirectional replication forks in replication initiation. On the other hand, the Taz1-dependent late replication origins that are visualized by lacO/LacI-GFP are localized at the nuclear periphery throughout the interphase, and associate with the telomeres specifically in the G1/S phase. The shelterin components play roles in tethering of the internal replication origins to the telomeres. The telomeric association of the replication origins causes suppression of early initiation by Rif1 and PP1 that are enriched at the telomeres.

Research Products

• [Journal Article] Nucleosomes around a mismatched base pair are excluded via an Msh2-dependent reaction with the aid of SNF2-family ATPase Smarcad1. (2018)
  • Author(s): Terui,R.,Nagao,K.,Kawasoe, Y., Taki,K., Higashi,TL., Tanaka,S., Nakagawa,T., Obusae,C., Masukata,H., Takahashi,TS.
  • Journal Title: Genes and Development Volume: -
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Regulation of mitotic recombination between DNA repeats in centromeres. (2017)
  • Author(s): Zafar,F., Okita,AK. Onaka,AT., Su,J., Katahira,Y., Nakayama,J. Takahashi,TS., Masukata, H., Nakagawa, T.
  • Journal Title: Nucleic Acids Research Volume: 44(22) Issue: 19 Pages: 11222-11235
  • DOI: 10.1093/nar/gkx763
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] MutSα maintains the mismatch repair capability by inhibiting PCNA unloading (2016)
  • Author(s): Kawasoe, Y., Tsurimoto, T., Nakagawa, T., Masukata, H., Takahashi, TS.
  • Journal Title: eLIFE Volume: 12;5
  • DOI: 10.7554/elife.15155
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Rad51 and Rad54 promote noncrossover recombination between centromere repeats on the same chromatid to prevent isochromosome formation. (2016)
  • Author(s): Onaka, AT., Toyofuku, N., Inoue, T., Okita, AK., Sagawa, M., Su, J., Shitanda, T., Matsuyama, R., Zafar, F., Takahashi, TS., Masukata, H., Nakagawa, T.
  • Journal Title: Nucleic Acids Research Volume: 44 (22) Issue: 22 Pages: 10744-10757
  • DOI: 10.1093/nar/gkw874
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Shugoshin forms a specialized chromatin domain at subtelomeres that regulates transcription and replication timing. (2016)
  • Author(s): Tashiro S, Handa T, Matsuda A, Ban T, Takigawa T, Miyasato K, Ishii K, Kugou K, Ohta K, Hiraoka Y, Masukata H, Kanoh J.
  • Journal Title: Nature Commun. Volume: 7 Issue: 1 Pages: 10393-10393
  • DOI: 10.1038/ncomms10393
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Histone H4 acetylation required for chromatin decompaction during DNA replication (2015)
  • Author(s): Ruan K, Yamamoto TG, Asakawa H, Chikashige Y, Kimura H, Masukata H, Haraguchi T, Hiraoka Y.
  • Journal Title: Sci Rep Volume: 5 Issue: 1 Pages: 1-10
  • DOI: 10.1038/srep12720
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Meiotic nuclear movements in fission yeast are regulated by the transcription factor Mei4 downstream of a Cds1-dependent replication checkpoint pathway. (2014)
  • Author(s): Ruan K, Yamamoto TG, Asakawa H, Chikashige Y, Masukata H, Haraguchi T, Hiraoka Y.
  • Journal Title: Genes Cells Volume: 20 Issue: 3 Pages: 160-172
  • DOI: 10.1111/gtc.12207
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Spatial and temporal control of chromosomal DNA replication by telomere sequence and telomere binding proteins in the fission yeast nucleus (2017)
  • Author(s): 升方久夫
  • Organizer: The 9th International Fission Yeast Meeting.
  • Int'l Joint Research
• [Presentation] Spatial and temporal control of chromosome DNA replication by telomere binding proteins. (2017)
  • Author(s): 小川志帆、城戸沙友里、小川英知、淺川東彦、高橋達郎、中川拓郎、平岡泰、升方久夫
  • Organizer: Eukaryotic DNA Replication and Genome Maintenance
  • Int'l Joint Research
• [Presentation] テロメア結合タンパク質およびシェルタリン構成因子によるDNA複製の時空間的制御機構の解明 (2017)
  • Author(s): 小川志帆、半田哲也、中村優太、高橋達郎、中川拓郎、小川英知、淺川東彦、平岡泰、升方久夫
  • Organizer: 第40回日本分子生物学会年会
• [Presentation] 分裂酵母シェルタリンによる複製開始タイミング制御機構 (2017)
  • Author(s): 城戸沙友里、小川志帆、高橋達郎、中川拓郎、升方久夫
  • Organizer: 第40回日本分子生物学会年会
• [Presentation] 分裂酵母における複製開始複合体形成の核内局在による制御 (2016)
  • Author(s): 升方久夫
  • Organizer: 第38回日本分子生物学会年会ワークショップ
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2016-11-30
  • Invited
• [Presentation] Control of DNA replication timing and location by telomere-binding proteins in fission yeast (2016)
  • Author(s): 升方久夫
  • Organizer: 10th 3R International Symposium
  • Place of Presentation: 松江
  • Year and Date: 2016-11-13
  • Int'l Joint Research / Invited
• [Presentation] Control of subnuclear localization and replication timing by telomere binding proteins in fission yeast (2016)
  • Author(s): 升方久夫
  • Organizer: FASEB Science Research Conferences
  • Place of Presentation: Snowmass, Colorado, USA
  • Year and Date: 2016-07-31
  • Int'l Joint Research / Invited
• [Presentation] Control of replication timing and subnuclear localization by telomere binding proteins in fission yeast (2016)
  • Author(s): 升方久夫
  • Organizer: DNA Metabolism, Genomic Stability and Disease、Cold Spring Harbor Asia
  • Place of Presentation: Suzhou、China
  • Year and Date: 2016-06-13
  • Int'l Joint Research / Invited
• [Presentation] テロメア結合タンパク質によるDNA複製の時空間的制御機構 (2015)
  • Author(s): 小川志帆、中村優太、高橋達郎、中川拓郎、小川英知、淺川東彦、平岡泰、升方久夫
  • Organizer: 第38回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2015-12-01
  • Invited
• [Presentation] DNA複製とミスマッチ修復の機能的相関 (2015)
  • Author(s): 高橋達郎、河添好孝、釣本敏樹、中川拓郎、升方久夫
  • Organizer: 第38回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2015-12-01
  • Invited
• [Presentation] Replication timing control by telomere-adjacent nuclear localization. (2015)
  • Author(s): 小川志帆、中村優太、半田哲也、小川英知、淺川東彦、高橋達郎、中川拓郎、平岡泰、升方久夫
  • Organizer: Eukaryotic DNA Replication and Genome Maintenance
  • Place of Presentation: Cold Spring Harbor, NY USA
  • Year and Date: 2015-09-01
  • Int'l Joint Research
• [Presentation] The mechanism of the MutSα-dependent strand memory reaction in Xenopus egg extract. (2015)
  • Author(s): 高橋達郎、河添好孝、釣本敏樹、中川拓郎、升方久夫
  • Organizer: Eukaryotic DNA Replication and Genome Maintenance
  • Place of Presentation: Cold Spring Harbor, NY USA
  • Year and Date: 2015-09-01
  • Int'l Joint Research
• [Presentation] Replication timing control by telomere-adjacent nuclear localization in fission yeast. (2015)
  • Author(s): 升方久夫、中村優太、小川英知、淺川東彦、高橋達郎、中川拓郎、平岡泰、小川志帆
  • Organizer: 第８回International Fission Yeast Meeting
  • Place of Presentation: 神戸
  • Year and Date: 2015-06-21
  • Int'l Joint Research / Invited
• [Presentation] Shugoshin forms a specialized chromatin domain at subtelomeres, repressing transcription and replication. (2015)
  • Author(s): 田代三喜、半田哲也、松田厚志、坂琢人、瀧川徹、宮里和実、石井浩二郎、久郷和人、太田邦広、平岡泰、升方久夫、加納純子
  • Organizer: 第８回International Fission Yeast Meeting
  • Place of Presentation: 神戸
  • Year and Date: 2015-06-21
  • Int'l Joint Research
• [Presentation] H3K9 methylation in heterochromatin suppresses gross chromosomal rearrangement in centromere in mitotic cells. (2015)
  • Author(s): 中川拓郎、沖田暁子、高橋達郎、升方久夫
  • Organizer: 第８回International Fission Yeast Meeting
  • Place of Presentation: 神戸
  • Year and Date: 2015-06-21
  • Int'l Joint Research
• [Presentation] Temporal and spatial regulation of replication in fission yeast. (2015)
  • Author(s): 小川志帆、中村優太、高橋達郎、中川拓郎、小川英知、淺川東彦、平岡泰、升方久夫
  • Organizer: 第８回International Fission Yeast Meeting
  • Place of Presentation: 神戸
  • Year and Date: 2015-06-21
  • Int'l Joint Research
• [Presentation] Rad51-independent recombination between centromere repeats causes gross chromosomal rearrangement in fission yeast. (2015)
  • Author(s): 大仲惇司、片平泰弘、沖田暁子、浅井麗伊、高橋達郎、升方久夫、中川拓郎
  • Organizer: 第８回International Fission Yeast Meeting
  • Place of Presentation: 神戸
  • Year and Date: 2015-06-21
  • Int'l Joint Research
• [Presentation] CENP-T histone-fold protein suppresses translocation in fission yeast. (2015)
  • Author(s): 蘇傑、高橋達郎、升方久夫、中川拓郎
  • Organizer: 第８回International Fission Yeast Meeting
  • Place of Presentation: 神戸
  • Year and Date: 2015-06-21
  • Int'l Joint Research
• [Remarks] 大阪大学大学院理学研究科生物科学専攻升方研究室
  • URL: http://www.bio.sci.osaka-u.ac.jp/dbs01/re-paper-temp.php?id=14
• [Remarks] 大阪大学大学院理学研究科生物科学専攻升方研究室
  • URL: http://www.bio.sci.osaka-u.ac.jp/bio_web/lab_page/masukata/thesis/index.html
• [Remarks] 大阪大学大学院理学研究科生物科学専攻升方研究室
  • URL: http://www.bio.sci.osaka-u.ac.jp/bio_web/lab_page/masukata/index.html
• [Remarks] 大阪大学大学院理学研究科生物科学専攻分子遺伝学研究室
  • URL: http://www.bio.sci.osaka-u.ac.jp/dbs01/re-paper-temp.php?id=14
• [Remarks] 大阪大学大学院理学研究科生物科学専攻分子遺伝学升方研究室ホームページ
  • URL: http://www.bio.sci.osaka-u.ac.jp/bio_web/lab_page/masukata/