Discovery of novel sulfated bioregulatory molecules acting on neural tissue receptors

• Project/Area Number: 15H04502
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Food science
• Research Institution: University of Miyazaki
• Principal Investigator: SAKAKIBARA YOICHI 宮崎大学, 農学部, 教授 (90295197)
• Co-Investigator(Kenkyū-buntansha): 水光 正仁 宮崎大学, 理事・副学長 (00128357) ; 榊原 啓之 宮崎大学, 農学部, 教授 (20403701)
• Project Period (FY): 2015-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000); Fiscal Year 2018: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2017: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2016: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2015: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
• Keywords: 硫酸転移酵素 / 硫酸化 / レセプター / 生体制御分子 / sulfation / sulfotransferase / sulfated / GPCR / 生体制御分子硫酸体 / 神経

Outline of Final Research Achievements

In this study, we focused on the functional control of bioregulatory molecules by sulfation as a metabolic mechanism. First, enzymatic preparation method of sulfated bioregulatory molecules were developed. Furthermore, we conducted research aimed at developing analysis technology for cell surface receptor-mediated signal transduction and elucidating its physiological function in cultured cells. Specifically, using E. coli that expressing human cytosolic sulfotransferase, we have established a biotechnology method to prepare a sulfated metabolite of a bioregulatory molecules such as food components. The obtained sulfated metabolite confirmed the chemical structure by NMR. Furthermore, a luciferase assay to evaluate GPR30 receptor-mediated activity was established, and the sulfated metabolites were evaluated for functional analysis.

Academic Significance and Societal Importance of the Research Achievements

現在、市場に流通する医薬品のほとんどがGタンパク質共役受容体を介して作用すると言われ、生体制御分子（ホルモン、食品機能性成分、医薬品など）の多くが受容体を標的として作用する。さらに、これら生体制御分子の多くは、肝臓等で代謝され、その代謝産物が神経細胞などで受容体に作用し機能していることが想定される。本研究は、硫酸化を受けた代謝物が受容体を介して細胞に作用することを確認した。このことは、硫酸化された代謝物は不活性な状態として排泄されているという定説を覆す知見となる。さらに、硫酸化代謝物に新規機能性を開発することで医薬品等の候補分子としての優位性を示すことが可能となる。

Research Products

• [Int'l Joint Research] トレド大学(米国)
• [Int'l Joint Research] トレド大学(米国)
• [Int'l Joint Research] トレド大学(米国)
• [Journal Article] Effects of human sulfotransferase 2A1 genetic polymorphisms 3 on the sulfation of Tibolone. (2018)
  • Author(s): Miller, E., Zalzala, M.H., Abunnaja, M.S, Kurogi, K., Sakakibara, Y., Suiko, M., Liu, M.-C.
  • Journal Title: European Journal of Drug Metabolism and Pharmacokinetics Volume: - Issue: 4 Pages: 415-421
  • DOI: 10.1007/s13318-017-0458-2
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Sulfation of catecholamines and serotonin by SULT1A3 allozymes. (2018)
  • Author(s): Bairam, A.F., Rasool, M.I., Alherz, F.A., Abunnaja, M.S., El Daibani, A.A., Gohal, S.A., Kurogi, K., Sakakibara, Y., Suiko, M., Liu, M.-C.
  • Journal Title: Biochamical Pharmacology Volume: 151 Pages: 104-113
  • DOI: 10.1016/j.bcp.2018.03.005
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Radical scavenging effects of 1-naphthol, 2-naphthol, and their sulfate-conjugates (2018)
  • Author(s): Sugahara S, Fukuhara K, Tokunaga Y, Tsutsumi S, Ueda Y, Ono M, Kurogi K, Sakakibara Y, Suiko M, Liu M-C, Yasuda S.
  • Journal Title: The Journal of Toxicological Sciences Volume: 43 Issue: 3 Pages: 213-221
  • DOI: 10.2131/jts.43.213
  • NAID: 130006507410
  • ISSN: 0388-1350, 1880-3989
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Effects of genetic polymorphisms on the sulfation of dehydroepiandrosterone and pregnenolone by human cytosolic sulfotransferase SULT2A1 (2018)
  • Author(s): Abunnaja, M.S., Alherz, F.A., El, Daibani, A.A., Bairam, A.F., Rasool, M.I., Gohal, S.A., Kurogi, K., Sakakibara, Y., Suiko, M., Liu, M.-C.
  • Journal Title: Biochemistry and Cell Biology Volume: 96 Issue: 5 Pages: 655-662
  • DOI: 10.1139/bcb-2017-0341
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The critical role of His48 in mouse cytosolic sulfotransferase SULT2A8 for the 7alfa-hydroxyl sulfation of bile acids (2018)
  • Author(s): Shimohira, T., Kurogi, K., Liu, M.-C., Suiko, M., Sakakibara, Y.
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 82 Issue: 8 Pages: 1359-136
  • DOI: 10.1080/09168451.2018.1464897
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] On the role of genetic polymorphisms in the cholesterol by human cytosolic sulphotransferase SULT2B1b (2018)
  • Author(s): Alherz, F.A., Daibani, A.A., Bairam, A.F., Abunnaja, M.S., El, Rasool, M.I., Kurogi, K., Sakakibara, Y., Suiko, M., Liu, M.-C.
  • Journal Title: Journal of Biochemistry Volume: 164 Issue: 3 Pages: 215-221
  • DOI: 10.1093/jb/mvy042
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Identification of zebrafish steroid sulfatase and comparative analysis of the enzymatic properties with human steroid sulfatase (2018)
  • Author(s): Kurogi, K., Yoshihama, M., Williams, F.E., Kenmochi, N., Sakakibara, Y., Suiko, M., Liu, M.-C.
  • Journal Title: The Journal of Steroid Biochemistry and Molecular Biology Volume: 185 Pages: 110-117
  • DOI: 10.1016/j.jsbmb.2018.08.004
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Identification and characterization of the zebrafish glutathione S-transferase Pi-1. (2017)
  • Author(s): Abunnaja, M. S., Kurogi, K., Mohammed, Y. I., Sakakibara, Y., Suiko, M., Hassoun, E., Liu, M.-C.
  • Journal Title: Journal of Biochemical and Molecular Toxicology Volume: 31 Issue: 10
  • DOI: 10.1002/jbt.21948
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Identification and characterization of the responsible human cytosolic sulfotransferases. (2017)
  • Author(s): Kurogi, K., Sakakibara, Y., Suiko, M., Liu, M.-C.
  • Journal Title: Federation of European Biochemical Societies Letters Volume: 591 Issue: 16 Pages: 2417-2425
  • DOI: 10.1002/1873-3468.12767
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Δ4-3-Ketosteroids as a new class of substrates for the cytosolic sulfotransferases. (2017)
  • Author(s): Hashiguchi, T., Kurogi, K., Shimohira, T., Teramoto, T., Liu, M.-C., Suiko, M., Sakakibara, Y.10.1016/j.bbagen.2017.08.005.
  • Journal Title: Biochimica et Biophysica Acta Volume: 1861 Issue: 11 Pages: 2883-2890
  • DOI: 10.1016/j.bbagen.2017.08.005
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Identification and characterization of 5alpha-cyprinol-sulfating cytosolic sulfotransferases (Sults) in the Zebrafish (Danio rerio). (2017)
  • Author(s): Kurogi, K., Yoshihama, M., Horton, A., Schiefer, I.T., Krasowski, M.D., Hagey, L.R., Williams, F.E., Sakakibara, Y., Kenmochi, N., Suiko, M., Liu, M.-C.
  • Journal Title: The Journal of Steroid Biochemistry and Molecular Biology Volume: 174 Pages: 120-127
  • DOI: 10.1016/j.jsbmb.2017.08.005
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Structural basis for the broad substrate specificity of the human tyrosylprotein sulfotaransferase-1. (2017)
  • Author(s): Tanaka, S., Nishiyori, T., Kojo, H., Otsubo, R., Tsuruta, M., Kurogi, K., Liu, M.-C., Suiko, M., Sakakibara, Y., Kakuta, Y.
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 8776-8776
  • DOI: 10.1038/s41598-017-07141-8
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Human cytosolic sulphotransferase SULT1C3: genomic analysis and functional characterization of splice variant SULT1C3 and SULT1C3d. (2017)
  • Author(s): Kurogi, K., Shimohira, T., Kouriki-Nagatomo, H., Zhang, G., Miller, E.R., Sakakibara, Y., Suiko, M., Liu, M.-C.
  • Journal Title: Journal of Biochemistry Volume: 162 Issue: 6 Pages: 403-414
  • DOI: 10.1093/jb/mvx044
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Regioselective production of sulfated polyphenols using human cytosolic sulfotransferase (SULT)-expressing Escherichia coli cells. (2017)
  • Author(s): Shimohira, T., Kurogi, K., Hashiguchi, T., Liu, M.-C., Suiko, M., Sakakibara, Y.
  • Journal Title: J. Biosci. Bioeng. Volume: - Issue: 1 Pages: 84-90
  • DOI: 10.1016/j.jbiosc.2017.02.006
  • NAID: 40022422358
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] A substrate-bound structure of cyanobacterial biliverdin reductase identifies stacked substrates as critical for activity (2017)
  • Author(s): Haruna Takao, Kei Hirabayashi, Yuki Nishigaya, Haruna Kouriki, Tetsuko Nakaniwa, Yoshinori Hagiwara, Jiro Harada, Hideaki Sato, Toshimasa Yamazaki, Yoichi Sakakibara, Masahito Suiko, Yujiro Asada, Yasuhiro Takahashi, Ken Yamamoto, Keiichi Fukuyama, Masakazu Sugishima, and Kei Wada
  • Journal Title: Nat. Commun. Volume: 8 Issue: 1 Pages: 1-10
  • DOI: 10.1038/ncomms14397
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Updated perspectives on the cytosolic sulfotransferases (SULTs) and SULT-mediated sulfation. (2017)
  • Author(s): Suiko, M., Kurogi, K., Hashiguchi, T., Sakakibara, Y., Liu, M.-C.
  • Journal Title: Biosci. Biotechnol. Biochem. Volume: 81 Issue: 1 Pages: 63-72
  • DOI: 10.1080/09168451.2016.1222266
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Human Cytosolic Sulfotransferase SULT1A3 Mediates the Sulfation of Dextrorphan (2016)
  • Author(s): Yamamoto, A., Kurogi, K., Schiefer, I.T., Liu, M.-Y., Sakakibara, Y., Suiko, M., Liu, M.-C.
  • Journal Title: Biological and Pharmaceutical Bulletin Volume: 39 Issue: 9 Pages: 1432-1436
  • DOI: 10.1248/bpb.b16-00015
  • NAID: 130005262319
  • ISSN: 0918-6158, 1347-5215
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Identification and Characterization of the Human Cytosolic Sulfotransferases Mediating the Sulfation of Clioquinol and Iodoquinol (2016)
  • Author(s): Yamamoto, A., Debrah-Pinamang, M., DiModica, N.J., Kurogi, K., Naqvi, A.A., Hui, Y., Sakakibara, Y., Suiko, M., Liu, M.-C.
  • Journal Title: Drug Metab. Lett. Volume: 10 Pages: 200-205
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] 植物硫酸転移酵素の諸性質と今後の展開 (2016)
  • Author(s): 橋口拓勇, 榊原陽一, 黒木勝久, 水光正仁
  • Journal Title: 日本農薬学会誌 Volume: 41 Pages: 198-202
  • NAID: 130005402046
  • Peer Reviewed
• [Journal Article] A novel procedure for the assessment of the antioxidant capacity of food components. (2016)
  • Author(s): Yoshimura, T., Harashima, M., Kurogi, K., Suiko, M., Liu, M.-C., Sakakibara, Y.
  • Journal Title: Anal. Biochem. Volume: 507 Pages: 7-12
  • DOI: 10.1016/j.ab.2016.05.002
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Sulfate Conjugation of Daphnetin by the Human Cytosolic Sulfotransferases. (2016)
  • Author(s): Han, Z., Xi, Y., Luo, L., Zhou, C., Kurogi, K., Sakakibara, Y., Suiko, M., Liu, M.-C.
  • Journal Title: J. Ethnopharmacol. Volume: S0378-8741 Pages: 30311-30317
  • DOI: 10.1016/j.jep.2016.05.041
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Human cytosolic sulfotransferase SULT1C4 mediates the sulfation of doxorubicin and epirubicin. (2016)
  • Author(s): Luo L, Zhou C, Hui Y, Kurogi K, Sakakibara Y, Suiko M, Liu MC.
  • Journal Title: Drug Metab. Pharmacokinet. Volume: 31 Issue: 2 Pages: 163-166
  • DOI: 10.1016/j.dmpk.2016.01.003
  • NAID: 50014431257
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Sulfation of Acetaminophen by the Human Cytosolic Sulfotransferases: A Systematic Analysis. (2015)
  • Author(s): Yamamoto, A., Liu, M.-Y., Kurogi, K., Sakakibara, Y., Saeki, Y., Suiko, M., Liu, M.-C.
  • Journal Title: The Journal of Biochemistry Volume: 158 Pages: 497-504
  • DOI: 10.1093/jb/mvv062
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Sulfation of afimoxifene, endoxifen, raloxifene, and fulvestran by the human cytosolic sulfotransferases (SULTs): A system analysis. (2015)
  • Author(s): Hui, Y., Luo, L., Zhang, L., Kurogi, K., Zhou, C., Sakakibara, Y., Suiko, M., Liu, M.-C.
  • Journal Title: Journal of Pharmacological Sciences Volume: 128 Issue: 3 Pages: 144-149
  • DOI: 10.1016/j.jphs.2015.06.004
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Sulfation of 6-Gingerol by the Human Cytosolic Sulfotransferases: A Systematic Analysis. (2015)
  • Author(s): Luo, Y., Mei, X., Xi, Y., Zhou, C., Hui, Y., Kurogi, K., Sakakibara, Y., Suiko, M., Liu, M.-C.
  • Journal Title: Planta Medica Volume: 82 Issue: 03 Pages: 238-243
  • DOI: 10.1055/s-0035-1558158
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Sulfation of 6-hydroxymelatonin, N-acetylserotonin and 4-hydroxyramelteon by the human cytosolic sulfotransferases (SULTs) (2015)
  • Author(s): Luo, L., Zhou, C., Kurogi, K., Sakakibara, Y., Suiko, M., Liu, M.-C.
  • Journal Title: Xenobiotica Volume: 46 Issue: 7 Pages: 612-619
  • DOI: 10.3109/00498254.2015.1107656
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Sulfation of benzyl alcohol by the human cytosolic sulfotransferases (SULTs): a systematic analysis. (2015)
  • Author(s): Zhang L, Kurogi K, Liu MY, Schnapp AM, Williams FE, Sakakibara Y, Suiko M, Liu MC.
  • Journal Title: J. Appl. Toxicol. Volume: none Issue: 9 Pages: 1090-1094
  • DOI: 10.1002/jat.3268
  • Peer Reviewed / Int'l Joint Research
• [Presentation] シクロペンテノンプロスタグランジン代謝体の受容体を介する作用解析 (2018)
  • Author(s): 児玉直輝、黒木勝久、Ming-Cheh Liu、水光正仁、榊原陽一
  • Organizer: 日本生化学会九州支部例会
• [Presentation] コモンマーモセット硫酸転移酵素SULT1Cサブファミリーに関する研究 (2018)
  • Author(s): 眞鍋曜子、黒木勝久、Ming-Cheh Liu、水光正仁、榊原陽一
  • Organizer: 日本生化学会九州支部例会
• [Presentation] 脂質由来α,β-不飽和アルデヒドおよびカルボニル化合物の硫酸化に関する研究 (2018)
  • Author(s): 黒木勝久、児玉直輝、水光正仁、榊原陽一
  • Organizer: 第42回蛋白質と酵素の構造と機能に関する九州シンポジウム
• [Presentation] 食事性タンパク質由来代謝物indoxyl sulfateの好中球細胞酸化促進作用 (2018)
  • Author(s): 堤秀平、黒木勝久、榊原陽一、水光正仁、安田伸
  • Organizer: 日本農芸化学会2018年度西日本支部大会
• [Presentation] 小胞体ストレスを軽減する食品成分の探索 (2017)
  • Author(s): 杉尾里和、下平武彦、神力はるな、永浜清子、近藤知巳、水光正仁、黒木勝久、榊原陽一
  • Organizer: 日本生化学会九州支部例会
• [Presentation] 肥育ホルモン硫酸化活性を有するbovine SULTの同定 (2017)
  • Author(s): 石川和希、吉瀬仁宣、下平武彦、水光正仁、黒木勝久、榊原陽一
  • Organizer: 日本生化学会九州支部例会
• [Presentation] PAPS合成酵素アイソフォームの生理機能解明 (2017)
  • Author(s): 上中勝護、山下光恵、黒木勝久、上地珠代、剣持直哉、Ming-Cheh Liu、水光正仁、榊原陽一
  • Organizer: 日本プロテオーム学会2017年大会
• [Presentation] プロスタグランジン代謝物のプロスタグランジン受容体への作用解析 (2017)
  • Author(s): 黒木勝久、榊原陽一、Ming-Cheh Liu、水光正仁
  • Organizer: 日本農芸化学会関西・中四国・西日本支部2017年度合同大阪大会
• [Presentation] PAPS合成酵素アイソフォームの生理機能解明 (2017)
  • Author(s): 上中勝護、山下光恵、黒木勝久、上地珠代、剣持直哉、Ming-Cheh Liu、水光正仁、榊原陽一
  • Organizer: 日本電気泳動学会総会
• [Presentation] Preparation and Functions of Regiospecific Sulfated Polyphenols (2016)
  • Author(s): Takehiko Shimohira, Katsuhisa Kurogi, Ming-Cheh Liu, Yoichi Sakakibara, Masahito Suiko
  • Organizer: YABEC2016
  • Place of Presentation: Phenix Seagaia Resort (Miyazaki)
  • Year and Date: 2016-10-27
  • Int'l Joint Research
• [Presentation] 生体制御分子硫酸体の生理機能評価技術開発 (2016)
  • Author(s): 下平武彦、黒木勝久、Ming-Cheh Liu、榊原陽一、水光正仁
  • Organizer: 第68回日本生物工学会大会
  • Place of Presentation: 富山国際会議場（富山）
  • Year and Date: 2016-09-28
• [Presentation] ゼブラフィッシュをモデルとしたPAPS合成酵素の生理機能解明 (2016)
  • Author(s): 上中勝護、上地珠代、剣持直哉、黒木勝久、Ming-Cheh Liu、水光正仁、榊原陽一
  • Organizer: 第89回日本生化学会大会
  • Place of Presentation: 仙台国際センター（仙台）
  • Year and Date: 2016-09-25
  • Invited
• [Presentation] 生体制御分子硫酸体の受容体を介した機能発現メカニズム解明を目指して (2016)
  • Author(s): 下平武彦、黒木勝久、Ming-Cheh Liu、水光正仁、榊原陽一
  • Organizer: 第４０回蛋白質と酵素の構造と機能に関する九州シンポジウム
  • Place of Presentation: 指宿ベイテラス（指宿）
  • Year and Date: 2016-08-26
• [Presentation] Sulfation of naphthoquinone and structurally related compounds (2016)
  • Author(s): Yoichi Sakakibara, Tomoki Takeyasu, Katsuhisa Kurogi, Ming-Cheh Liu, Masahito Suiko
  • Organizer: Conjugation 2016
  • Place of Presentation: UBC (Vancouver, Canada)
  • Year and Date: 2016-06-19
  • Int'l Joint Research
• [Presentation] フラボノイドおよびフラボノイド硫酸体結合タンパク質の探索 (2016)
  • Author(s): 小川穂乃香、下平武彦、黒木勝久、Ming-Cheh Liu、水光正仁、榊原陽一
  • Organizer: 日本生化学会九州支部例会
  • Place of Presentation: 鹿児島大学
  • Year and Date: 2016-05-14
• [Presentation] ウシ肝臓膜結合型フェノール硫酸転移酵素の同定 (2016)
  • Author(s): 吉瀬仁宣、黒木勝久、Ming-Cheh Liu、水光正仁、榊原陽一
  • Organizer: 日本生化学会九州支部例会
  • Place of Presentation: 鹿児島大学（鹿児島）
  • Year and Date: 2016-05-14
• [Presentation] ケトステロイドの代謝機構としての硫酸化 (2015)
  • Author(s): 橋口拓勇、黒木勝久、Liu, Ming-Cheh、水光正仁、榊原陽一
  • Organizer: BMB2015
  • Place of Presentation: 神戸ポートアイランド（兵庫・神戸）
  • Year and Date: 2015-12-01
• [Presentation] PAPS合成酵素アイソフォームの機能解明とその利用 (2015)
  • Author(s): 上中勝護、黒木勝久、上地珠代、剣持直哉、Liu, Ming-Cheh、水光正仁、榊原陽一
  • Organizer: 第67回日本生物工学会大会
  • Place of Presentation: 城山観光ホテル（鹿児島・鹿児島）
  • Year and Date: 2015-10-26
• [Presentation] プロテオミクスによるゼブラフィッシュPAPS合成酵素の機能解明 (2015)
  • Author(s): 上中勝護、上地珠代、黒木勝久、剣持直哉、Liu, Ming-Cheh、水光正仁、榊原陽一
  • Organizer: 日本農芸化学会2015年度中四国・西日本支部合同大会
  • Place of Presentation: 愛媛大学（愛媛・松山）
  • Year and Date: 2015-09-18
• [Presentation] ヒト蛋白質チロシン硫酸転移酵素の結晶構造に基づく硫酸化機構解明 (2015)
  • Author(s): 田中槙之助、西依利晃、古城英貴、坂上功樹、黒木勝久、榊原陽一、水光正仁、木村誠、角田佳充
  • Organizer: 日本農芸化学会2015年度中四国・西日本支部合同大会
  • Place of Presentation: 愛媛大学（愛媛・松山）
  • Year and Date: 2015-09-18
• [Presentation] 4-Methylumbelliferoneとその硫酸化代謝物による活性酸素産生抑制作用 (2015)
  • Author(s): 菅原進太郞、小野政輝、井越敬司、黒木勝久、榊原陽一、水光正仁、安田伸
  • Organizer: 2015年度生物機能研究会
  • Place of Presentation: 佐賀大学（佐賀・佐賀）
  • Year and Date: 2015-07-04
• [Presentation] 食品機能性成分硫酸体の受容体を介した機能発現メカニズム解明を目指して (2015)
  • Author(s): 下平武彦、黒木勝久、Liu, Ming-Cheh、榊原陽一、水光正仁
  • Organizer: 2015年度生物機能研究会
  • Place of Presentation: 佐賀大学（佐賀・佐賀）
  • Year and Date: 2015-07-04
• [Presentation] PAPS合成酵素アイソフォームの生理機能解明 (2015)
  • Author(s): 上中勝護、黒木勝久、上地珠代、剣持直哉、Liu, Ming-Cheh、水光正仁、榊原陽一
  • Organizer: 2015年度生物機能研究会
  • Place of Presentation: 佐賀大学（佐賀・佐賀）
  • Year and Date: 2015-07-04
• [Presentation] Identification and Cheracterization of Vitamin K Sulfotransferase (2015)
  • Author(s): Tomoaki Takeyasu, Katsuhisa Kurogi, Ming-Cheh Liu, Yoichi Sakakibara, Masahito Suiko
  • Organizer: ICCP450 Tokyo 2015
  • Place of Presentation: 国立オリンピック記念青少年センター（東京・渋谷）
  • Year and Date: 2015-06-13
  • Int'l Joint Research
• [Presentation] 脊椎動物PAPS合成酵素の機能解析 (2015)
  • Author(s): 上中勝護、黒木勝久、上地珠代、剣持直哉、Liu, Ming-Cheh、榊原陽一、水光正仁
  • Organizer: 平成27年度 日本生化学会九州支部例会
  • Place of Presentation: 九州大学（福岡・福岡）
  • Year and Date: 2015-05-16
• [Presentation] ヒト蛋白質チロシン硫酸転移酵素1の結晶構造解析 (2015)
  • Author(s): 田中槙之助、西依利晃、古城英貴、黒木勝久、榊原陽一、水光正仁、木村誠、角田佳充
  • Organizer: 平成27年度 日本生化学会九州支部例会
  • Place of Presentation: 九州大学（福岡・福岡）
  • Year and Date: 2015-05-16
• [Remarks] 宮崎大学農学部応用生物科学科 生体分子機能化学研究室
  • URL: http://biochemistrylab.web.fc2.com