Microbial degradation and detoxification of neonicotinoides by symbiotic systems of white-rot fungi with bacteria

• Project/Area Number: 15H04618
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental agriculture(including landscape science)
• Research Institution: Shizuoka University
• Principal Investigator: Hirai Hirofumi 静岡大学, 農学部, 教授 (70322138)
• Co-Investigator(Kenkyū-buntansha): 一瀬 博文 九州大学, 農学研究院, 准教授 (00432948) ; 長井 薫 甲子園大学, 栄養学部, 教授 (20340953) ; 亀井 一郎 宮崎大学, 農学部, 教授 (90526526)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000); Fiscal Year 2017: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2016: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2015: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
• Keywords: ネオニコチノイド / 白色腐朽菌 / 細菌 / バイオレメディエーション / 微生物

Outline of Final Research Achievements

Neonicotinoides (NEOs) have been widely used world-wide. However, its toxicity for bees and neurological toxicity for humans are urgent problems. In the present study, we clarified that white-rot fungi could degrade and detoxify NEOs, and that cytochrome P450 was involved in the degradation. Moreover, the symbiotic systems of white-rot fungi with bacteria effectively degraded NEOs.

Research Products

• [Journal Article] 白色腐朽菌を用いたネオニコチノイド系殺虫剤の分解・無毒化 (2018)
  • Author(s): 平井浩文
  • Journal Title: アグリバイオ Volume: 2 Pages: 53-55
  • NAID: 40021807548
• [Journal Article] Bioremediation of the neonicotinoid insecticide clothianidin by the white-rot fungus Phanerochaete sordida (2017)
  • Author(s): Toshio Mori, Jianqiao Wang, Yusuke Tanaka, Kaoru Nagai, Hirokazu Kawagishi, Hirofumi Hirai
  • Journal Title: Journal of Hazardous Materials Volume: 321 Pages: 586-590
  • DOI: 10.1016/j.jhazmat.2016.09.049
  • NAID: 120006501668
  • Peer Reviewed / Open Access
• [Presentation] 白色腐朽菌によるアセタミプリド分解に関与するシトクロムP450遺伝子の同定 (2018)
  • Author(s): 大野 遥、一瀬 博文、森 智夫、河岸 洋和、平井 浩文
  • Organizer: 日本農芸化学会2018年度大会
• [Presentation] Degradation of neonicotinoid insecticides by the white-rot fungus Phanerochaete sordida YK-624 (2017)
  • Author(s): Jianqiao Wang, Yusuke Tanaka, Toshio Mori, Hirokazu Kawagishi, Hirofumi Hirai
  • Organizer: The 14th International Symposium on Persistent Toxic Substances
  • Int'l Joint Research
• [Presentation] 高活性リグニン分解菌Phanerochaete sordida YK-624株によるネオニコチノイド系殺虫剤の分解及び無毒化 (2016)
  • Author(s): 田中佑典、王剣橋、長井薫、森智夫、河岸洋和、平井浩文
  • Organizer: 第61回リグニン討論会
  • Place of Presentation: 京都大学宇治キャンパス（京都府、宇治市）
  • Year and Date: 2016-10-27
• [Presentation] 地球環境問題解決に向けた担子菌の利用研究 (2016)
  • Author(s): 平井浩文
  • Organizer: 日本きのこ学会第20回大会 公開シンポジウム
  • Place of Presentation: 静岡県男女共同参画センター「あざれあ」（静岡県、静岡市）
  • Year and Date: 2016-09-07
  • Invited
• [Presentation] 白色腐朽菌Phanerochaete sordida YK-624株によるクロチアニジンの分解 (2016)
  • Author(s): 田中佑典、王剣橋、長井薫、森智夫、河岸洋和、平井浩文
  • Organizer: 第66回日本木材学会大会
  • Place of Presentation: 名古屋大学（愛知県名古屋市）
  • Year and Date: 2016-03-27
• [Book] きのこの生理機能と応用開発の展望 (2017)
  • Author(s): 平井浩文 他44名
  • Total Pages: 377
  • Publisher: S&T出版
  • ISBN: 9784907002664