| Project/Area Number |
15H04674
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General physiology
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| Research Institution | University of Fukui |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
竹内 綾子 福井大学, 学術研究院医学系部門, 准教授 (00378704)
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| Co-Investigator(Renkei-kenkyūsha) |
HIKIDA Masaki 秋田大学, 大学院理工学研究科, 教授 (60228715)
SHIMAYOSHI Takao 九州大学, 情報基盤研究開発センター, 准教授 (60373510)
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| Research Collaborator |
IINO Satoshi 福井大学, 学術研究院医学系部門, 教授 (40242854)
HORIGUCHI Kazuhide 福井大学, 学術研究院医学系部門, 准教授 (20377451)
FUKAZAWA Yugo 福井大学, 学術研究院医学系部門, 教授 (60343745)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2017: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2015: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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| Keywords | ミトコンドリア / カルシウム / 心臓 / 数理モデル / 心臓生理 / トランスポータ / 心臓生理学 / モデル |
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| Outline of Final Research Achievements |
NCLX-knockout mice were created using the CRISPR-Cas9 method. Some of the homo-knockout mice show phenotypes different form wild type mice, such as low body weight, and abnormalities of heart and kidney. The Langendorff perfusion of mouse heart with a NCLX blocker (CGP-37157) tended to have decreased heart rate. In isolated mouse ventricular myocytes, CGP-37157 prolonged the 30 and 50% duration of action potential, and augmented the amplitude of Ca2+ transient probably due to the increase of SR Ca2+ content. A new comprehensive mathematical model of cardiac mitochondria was developed and published. A new mathematical model of sinoatrial cell, which contains the Ca2+ interaction between mitochondria and SR, was developed.
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