Analysis of elimination of non-self and development of Stealth T cells aiming generalized antigen-specific adoptive T cell therapy
| Project/Area Number |
15H04918
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Nagasaki University (2016-2017)
Mie University (2015) |
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Principal Investigator |
IKEDA Hiroaki 長崎大学, 医歯薬学総合研究科(医学系), 教授 (40374673)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2017: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
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| Keywords | 細胞療法 / 遺伝子治療 / がん / 非自己細胞 / TCR / MHC / 癌 / T細胞輸注療法 / 免疫学 / がん免疫療法 / ゲノム編集 / siRNA |
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| Outline of Final Research Achievements |
We successfully generated 'Stealth T cells' that lack MHC class I expression as well as endogenous T cell receptor expression along with enforced expression of NY-ESO-1-specific T cell receptor utilizing beta 2 micro globulin-targetted CRISPR-Cas9 and siTCR vector. We showed that these 'Stealth T cells' lost the ability to stimulate allogeneic T cells while maintaining the anti-tumor activity against NY-ESO-1 positive tumors. These results suggested that 'Stealth T cells' will be useful for the adoptive T cell therapy with allogeneic T cells for cancer patients.
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Report
(4 results)
Research Products
(63 results)
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[Journal Article] Clinical Implications of CD4+CD25+Foxp3+Regulatory T Cell Frequencies After CHP-MAGE-A4 Cancer Vaccination2018
Author(s)
Wada M, Tsuchikawa T, Kyogoku N, Abiko T, Miyauchi K, Takeuchi S, Kuwatani T, Shichinohe T, Miyahara Y, Kageyama S, Ikeda H, Shiku H, Hirano S.
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Journal Title
Anticancer Research
Volume: 38
Issue: 3
Pages: 1435-1444
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Safety and persistence of WT1-specific T-cell receptor gene-transduced lymphocytes in patients with AML and MDS.2017
Author(s)
Tawara I, Kageyama S, Miyahara Y, Fujiwara H, Nishida T, Akatsuka Y, Ikeda H, Tanimoto K, Terakura S, Murata M, Inaguma Y, Masuya M, Inoue N, Kidokoro T, Okamoto S, Tomura D, Chono H, Nukaya I, Mineno J, Naoe T, Emi N, Yasukawa M, Katayama N, Shiku H.
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Journal Title
Blood
Volume: 130
Issue: 18
Pages: 1985-1994
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Differential detection of cytoplasmic Wilms tumor 1 expression by immunohistochemistry, Western blotting, and mRNA quantification.2017
Author(s)
Maki T, Ikeda H, Kuroda A, Kyogoku N, Yamamura Y, Tabata Y, Abiko T, Tsuchikawa T, HidaY, ShichinoheT, Tanaka E, Kaga K, Hatanaka K, MatsunoY, Imai N, Satoshi Hirano.
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Journal Title
International Journal of Oncology
Volume: 50
Issue: 1
Pages: 129-140
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Time-dependent transition of the immunoglobulin G subclass and immunoglobulin E response in cancer patients vaccinated with cholesteryl pullulan-melanoma antigen gene-A4 nanogel.2016
Author(s)
Kyogoku N, Ikeda H, Tsuchikawa T, Abiko T, Fujiwara A, Maki T, Yamamura Y, Ichinokawa M, Tanaka K, Imai N, Miyahara Y, Kageyama S, Shiku H, Hirano S.
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Journal Title
Oncology Letters
Volume: 12
Issue: 6
Pages: 4493-4504
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Clinical relevance of antigen spreading pattern induced by CHP-MAGE-A4 cancer vaccination2016
Author(s)
Miyauchi K, Tsuchikawa T, Wada M, Abiko T, Kyogoku N, Shichinohe T, Miyahara Y, Kageyama S, Ikeda H, Shiku H, Hirano S.
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Journal Title
Immunotherapy
Volume: 印刷中
Issue: 5
Pages: 527-40
DOI
Related Report
Peer Reviewed
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[Journal Article] Adoptive transfer of MAGE-A4 T-cell receptor gene-transduced lymphocytes in patients with recurrent esophageal cancer2015
Author(s)
Kageyama S, Ikeda H, Miyahara Y, Imai N, Ishihara M, Saito K, Sugino S, Ueda S, Ishikawa T, Kokura S, Naota H, Ohishi K, Shiraishi T, Inoue N, Tanabe M, Kidokoro T, Yoshioka H, Tomura D, Nukaya I, Mineno J, Takesako K, Katayama N, Shiku H.
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Journal Title
Clinical Cancer Research
Volume: in press
Issue: 10
Pages: 2268-2277
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] 2015 Guidance on cancer immunotherapy development in early-phase clinical studies2015
Author(s)
Guidance Development Review Committee; Working Group for Clinical Studies of Cancer Immunotherapy; Working Group for Effector Cell Therapy; Working Group for CMC/Non-clinical Studies; Working Group for Cancer Vaccines and Adjuvants; Working Group for Anti-immune Checkpoint Therapy and Comprehensive Cancer
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Journal Title
Cancer Sci
Volume: 106
Issue: 12
Pages: 1761-1771
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] Cytokine Release Syndrome and Tumor Responses in a First-in-Man Trial of a Novel Affinity-Enhanced TCR-Gene Transduced T Cell Transfer Targeting NY-ESO-1 Antigen2017
Author(s)
Kageyama S, Ishihara M, Kitano S, Miyahara Y, Kato H, Mishima H, Yamamoto N, Hattori H, Iwase H, Funakoshi T, Kojima T Inoue N, Kidokoro T, Tomura D, Okamoto S, Chono H, Nukaya I, Mineno J, Ikeda H, Watanabe T, Shiku H
Organizer
2017 米国血液学会総会
Related Report
Int'l Joint Research
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[Presentation] Cytokine release syndrome and tumor responses in a first-in-man trial of a novel affinity-enhanced TCR-gene transduced T cell transfer targeting NY-ESO-1 antigen2017
Author(s)
Hiroyoshi Hattori, Mikiya Ishihara, Shigehisa Kitano, Yoshihiro Miyahara, Hidefumi Kato, Hideyuki Mishima, Noboru Yamamoto, Hiroaki Iwase, Takeru Funakoshi, Takashi Kojima, Hiroaki Ikeda, Takashi Watanabe, , Shinichi Kageyama, Hiroshi Shiku
Organizer
2017 ESGCT
Related Report
Int'l Joint Research
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[Presentation] Clinical trials of NY-ESO-1 and MAGE-A4 targeting TCR-gene transduced T cell transfer to patients with refractory solid tumors2017
Author(s)
Shinichi Kageyama, Mikiya Ishihara, Shigehisa Kitano, Yoshihiro Miyahara, Hidefumi Kato, Hideyuki Mishima, Noboru Yamamoto, Hiroaki Iwase, Hiroyoshi Hattori, Takeru Funakoshi, Takashi Kojima, Hiroaki Ikeda, Takashi Watanabe, Hiroshi Shiku
Organizer
第２３回 日本遺伝子細胞治療学会学術集会
Related Report
Invited
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[Presentation] Efficacy and safety of immunotherapy with chimeric antigen receptor targeting WT1 and HLA-A24:02 pMHC complex2016
Author(s)
Hiroaki Ikeda, Yasushi Akahori, Motohiro Yoneyama, Yuki Orito, Yoshihiro Miyahara, Yasunori Amaishi, Sachiko Okamoto, Junichi Mineno, Kazutoh Takesako, Hiroshi Fujiwara, Masaki Yasukawa, Hiroshi Shiku
Organizer
米国遺伝子細胞治療学会
Place of Presentation
Washigton DC, USA
Year and Date
2016-05-04
Related Report
Int'l Joint Research
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[Presentation] Immunotherapy with chimeric antigen receptor targeting intracellular WT1 gene product complexed with HLA-A*24:02 molecule2015
Author(s)
Hiroaki Ikeda, Yasushi Akahori, Motohiro Yoneyama, Yuki Orito, Yoshihiro Miyahara, Yasunori Amaishi, Sachiko Okamoto, Junichi Mineno, Kazutoh Takesako, Hiroshi Fujiwara, Masaki Yasukawa, Hiroshi Shiku
Organizer
57th ASH Annual Meeting and Exposition
Place of Presentation
Orlando, USA
Year and Date
2015-12-05
Related Report
Int'l Joint Research
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[Presentation] Development of immunotherapy with chimeric antigen receptor targeting intracellular WT1 gene product presented on HLA-A*24:02 molecule2015
Author(s)
Yasushi Akahori, Motohiro Yoneyama, Hiroaki Ikeda, Yuki Orito, Yoshihiro Miyahara, Yasunori Amaishi, Sachiko Okamoto, Junichi Mineno, Kazutoh Takesako, Hiroshi Fujiwara, Masaki Yasukawa, Hiroshi Shiku
Organizer
がん免疫療法・マクロファージ国際会議 2015
Place of Presentation
東京大学（東京都・文京区）
Year and Date
2015-07-09
Related Report
Int'l Joint Research
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[Presentation] Donor lymphocyte infusion thrapy utilizing genetically engineered lymphocytes that express tumor-specific TCR and siRNA for endogenous TCR enables tumor eradication without GvHD induction.2015
Author(s)
Ikeda H, Ueno H, Tawara I, Kawamura A, Okamori K, Imai N, Okamoto S, Mineno J, Takesako K, Katayama N, Shiku H.
Organizer
13th Annual Meeting of Association of Cancer Immunotherapy (CIMT).
Place of Presentation
Mainz, Germany
Year and Date
2015-05-12
Related Report
Int'l Joint Research
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