Molecular mechanism for quality control of bacterial outer membrane by the periplasmic protease BepA
| Project/Area Number |
15K07008
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Morioka College |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
AKIYAMA Yoshinori 京都大学・ウイルス, 再生医科学研究所, 教授 (10192460)
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| Research Collaborator |
DAIMON Yasushi
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 細胞内タンパク質分解 / 表層ストレス応答 / 細菌細胞表層 / プロテアーゼ / シャペロン / 外膜タンパク質 / ジスルフィド結合 / タンパク質間相互作用 |
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| Outline of Final Research Achievements |
BepA is an Escherichia coli periplasmic protease involved in biogenesis and quality control of LptD, a component of the outer membrane lipopolysaccharide translocon. BepA can associate with the BAM complex driving integration of outer membrane proteins into the outer membrane. However, the molecular mechanism of BepA function and its association with the BAM complex remained unknown. In this study, we performed a systematic site-directed in vivo photo-cross-linking analysis to reveal the protein-protein interactions mediated by the BepA TPR domain. We revealed the mode of interaction between the BepA domein and the BAM complex as well as LptD. Mutational analysis indicated that BepA TPR domain is essential and protein-protein interactions mediated by the TPR domain is critical for the function of BepA.
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Report
(4 results)
Research Products
(9 results)
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[Journal Article] BepA mediates productive transfer of substrate proteins to the β-barrel assembly machinery (BAM) complex.2017
Author(s)
Daimon, Y., Masui, C., Tanaka, Y., Shiota, T., Suzuki, T., Miyazaki, R., Sakurada, H., Lithgow, T., Dohmae, N., Mori, H., Tsukazaki, T., Narita, S. and Akiyama, Y.
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Journal Title
Mol. Microbiol.
Volume: 106
Issue: 5
Pages: 760-776
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Novel antibacterial targets and compounds revealed by a high throughput cell wall reporter assay2015
Author(s)
Asha S. Nayar, Thomas J. Dougherty, Keith E. Ferguson, Brett A. Granger, Lisa McWilliams, Clare Stacey, Lindsey J. Leach, Shin-ichiro Narita, Hajime Tokuda, Alita A. Miller, Dean G. Brown, and Sarah M. McLeod
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Journal Title
J. Bacteriol.
Volume: 197
Issue: 10
Pages: 1726-1734
DOI
Related Report
Peer Reviewed
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