Production of novel prebiotics for intestinal bifidobacteria from food wastes
| Project/Area Number |
15K07448
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | Kindai University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | ビフィズス菌 / プロバイオティクス / プレバイオティクス / グリコシダーゼ / エステラーゼ |
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| Outline of Final Research Achievements |
The production of prebiotics for intestinal bifidobacteria from two food wastes was studied.
(1) By using bifidobacterial glycosidases or cells, bifidogenic disaccharide galacto-N-biose was released from wasted mucin, which contains abundant O-glycans. A novel 6-sulfo-N-acetylglucosaminidase, an essential enzyme for degradation of terminally sulfated glycans, was identified. A bifidobacterial strain defective in incorporation of galacto-N-biose was also screened, which is suitable for production of the disaccharide.
(2) Mume-polyphenols prepared from umesu, a waste of umeboshi production, was found to enhance bifidobacterial growth. The major phenolic compound, prunose, was assimilated by B. catenulatum and B. pseudocatenulatum, which express prunose-specific ferulate esterase. Arabinan-like polysaccharides was degraded by two novel GH43 exo-α-arabinofuranosidases from B. longum. Effects of orally administrated mume-polyphenols on intestinal microbiota was evaluated using mice.
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Report
(4 results)
Research Products
(34 results)
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[Journal Article] Chemo-enzymatic synthesis of the glucagon containing N-linked oligosaccharide and its characterization.2018
Author(s)
Higashiyama, T., Umekawa, M., Nagao, M., Katoh, T., Ashida, H., Yamamoto, K.
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Journal Title
Carbohydrate Research
Volume: 455
Pages: 92-96
DOI
Related Report
Peer Reviewed
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[Journal Article] The First Crystal Structure of a Family 129 Glycoside Hydrolase from a Probiotic Bacterium Reveals Critical Residues and Metal Co-factors2017
Author(s)
Mayo Sato, Dorothee Liebschner, Yusuke Yamada, Naohiro Matsugaki, Takatoshi Arakawa, Siobhan S. Wills, Mitchell Hattie, Keith A. Stubbs, Tasuku Ito, Toshiya Senda, Hisashi Ashida and Shinya Fushinobu
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Journal Title
Journal of Biological Chemistry
Volume: 292
Issue: 29
Pages: 12126-12138
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Introduction of H-antigens into oligosaccharides and sugar chains of glycoproteins using highly efficient 1,2-α-L-fucosynthase2016
Author(s)
Sugiyama Y, Gotoh A, Katoh T, Honda Y, Yoshida E, Kurihara S, Ashida H, Kumagai H, Yamamoto K, Kitaoka M, and Katayama T.
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Journal Title
Glycobiology
Volume: 26
Pages: 1235-1247
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Application study of 1,2-α-L-fucosynthase: Introduction of Fucα1-2Gal disaccharide structures on N-glycan, ganglioside, and xyloglucan oligosaccharide2016
Author(s)
Sugiyama Y, Katoh T, Honda Y, Gotoh A, Ashida H, Kurihara S, Yamamoto K, and Katayama T.
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Journal Title
Bioscience, Biotechnology, and Biochemistry
Volume: 81
Issue: 2
Pages: 283-291
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Novel substrate specificities of two lacto-N-biosidases towards β-linked galacto-N-biose-containing oligosaccharides of globo H, Gb5, and GA1.2015
Author(s)
Gotoh A, Katoh T, Sugiyama Y, Kurihara S, Honda Y, Sakurama H, Kambe T, Ashida H, Kitaoka M, Yamamoto K, Katayama T
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Journal Title
Carbohydr Res
Volume: 408
Pages: 18-24
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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