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Establishment of the new strategy with REIC/Dkk-3 against androgen independent prostate cancer.

Research Project

Project/Area Number 15K07754
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Veterinary medical science
Research InstitutionNippon Veterinary and Life Science University

Principal Investigator

Ochiai Kazuhiko  日本獣医生命科学大学, 獣医学部, 准教授 (30550488)

Co-Investigator(Kenkyū-buntansha) 呰上 大吾  日本獣医生命科学大学, 獣医学部, 准教授 (80453934)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords前立腺がん / REIC/Dkk-3 / SGTA / イヌ / ホルモン療法抵抗性 / ARシグナル伝達
Outline of Final Research Achievements

The pathological condition of canine prostate cancer resembles that of human androgen-independent prostate cancer. Both canine and human androgen receptor (AR) signalling are inhibited by overexpression of the dimerized co-chaperone SGTA, which is considered to cause the development of androgen-independency. REIC interferes with SGTA dimerization and rescues AR signalling. This study aimed to assess the effects of REIC and SGTA interactions on AR signalling in the canine androgen-independent prostate cancer cell line CHP-1.
MTH and Halo-tag PD assays showed that canine REIC interacted with SGTA and interfered with SGTA dimerization. Additionally, reporter assays revealed that canine REIC restored AR signalling. Therefore, we confirmed the interaction between canine SGTA and REIC, as well as their role in AR signalling. Our results suggest that this interaction might contribute to the development of a novel strategy for androgen-independent prostate cancer treatment.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (17 results)

All 2018 2017 2016 2015 Other

All Journal Article (7 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 7 results,  Open Access: 4 results,  Acknowledgement Compliant: 1 results) Presentation (9 results) Remarks (1 results)

  • [Journal Article] Functional alteration of canine isocitrate dehydrogenase 2 (IDH2) via an R174K mutation2018

    • Author(s)
      Kawakami S, Ochiai K, Kato Y, Michishita M, Hirama H, Obara R, Azakami D, Watanabe M, Omi T
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 80 Issue: 1 Pages: 85-91

    • DOI

      10.1292/jvms.17-0362

    • NAID

      130006321212

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] R132 mutations in canine isocitrate dehydrogenase 1 (IDH1) lead to functional changes.2018

    • Author(s)
      Kawakami S, Ochiai K, Azakami D, Kato Y, Michishita M, Morimatsu M, Ishiguro-Oonuma T, Onozawa E, Watanabe M, Omi T.
    • Journal Title

      Vet Res Commun

      Volume: 42(1) Issue: 1 Pages: 49-56

    • DOI

      10.1007/s11259-017-9707-8

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Canine REIC/Dkk-3 interacts with SGTA and restores androgen receptor signalling in androgen-independent prostate cancer cell lines2017

    • Author(s)
      Kato Yuiko、Ochiai Kazuhiko、Kawakami Shota、Nakao Nobuhiro、Azakami Daigo、Bonkobara Makoto、Michishita Masaki、Morimatsu Masami、Watanabe Masami、Omi Toshinori
    • Journal Title

      BMC Veterinary Research

      Volume: 13 Issue: 1 Pages: 170-170

    • DOI

      10.1186/s12917-017-1094-4

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Properties of the feline tumour suppressor reduced expression in immortalized cells (REIC/Dkk-3).2017

    • Author(s)
      Ochiai K, Oda H, Shono S, Kato Y, Sugihara S, Nakazawa S, Azakami D, Michishita M, Onozawa E, Bonkobara M, Sako T, Shun-Ai L, Ueki H, Watanabe M, Omi T.
    • Journal Title

      Vet Comp Oncol

      Volume: In press Issue: 4 Pages: 1181-1186

    • DOI

      10.1111/vco.12254

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Tumor suppressor REIC/DKK-3 and co-chaperone SGTA: Their interaction and roles in the androgen sensitivity2016

    • Author(s)
      Ochiai K, Morimatsu M, Kato Y, Ishiguro-Oonuma T, Udagawa C, Rungsuriyawiboon O, Azakami D, Michishita M, Ariyoshi Y, Ueki H, Nasu Y, Kumon H, Watanabe M, Omi T.
    • Journal Title

      Oncotarget

      Volume: 7 Issue: 3 Pages: 3283-3296

    • DOI

      10.18632/oncotarget.6488

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The canine prostate cancer cell line CHP-1 shows over-expression of the co-chaperone small glutamine-rich tetratricopeptide repeat-containing protein α2016

    • Author(s)
      Azakami, D., Nakahira, R., Kato, Y., Michishita, M., Kobayashi, M., Onozawa, E., Bonkobara, M., Kobayashi, M., Takahashi, K., Watanabe, M., Ishioka, K., Sako, T., Ochiai, K., and Omi, T.
    • Journal Title

      Veterinary and Compapative Oncology

      Volume: 印刷中 Issue: 2 Pages: 557-562

    • DOI

      10.1111/vco.12199

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Molecular cloning of canine co-chaperone small glutamine-rich tetratricopeptide repeat-containing protein α (SGTA) and investigation of its ability to suppress androgen receptor signalling in androgen-independent prostate cancer.2015

    • Author(s)
      Kato, Y., Ochiai, K., Michishita, M., Azakami, D., Nakahira, R., Morimatsu, M., Ishiguro-Oonuma, T., Yoshikawa, Y., Kobayashi, M., Bonkobara, M., Kobayashi, M., Takahashi, K., Watanabe, M., and Omi, T.
    • Journal Title

      The Vwterinary Journal

      Volume: 206 Issue: 2 Pages: 145-148

    • DOI

      10.1016/j.tvjl.2015.08.002

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] イヌIDH1ホモログのクローニングと機能解析2017

    • Author(s)
      川上翔太、落合和彦、道下正貴、近江俊徳
    • Organizer
      日本獣医臨床病理学会2017年大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] がん抑制遺伝子REIC/Dkk-3ネコホモログの構造と乳腺腫瘍に対するアポトーシス誘導能の解析2017

    • Author(s)
      落合和彦、杉原思穂、小田民美、川上翔太、平間日奈子、呰上大吾、近江俊徳
    • Organizer
      第160回日本獣医学会学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] IDH1イヌホモログの変異が機能におよぼす影響の検討2017

    • Author(s)
      川上翔太,落合和彦、尾原涼、道下正貴、近江俊徳
    • Organizer
      第160回日本獣医学会学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] CMAH遺伝子解析による猫 AB式血液型の分子基盤2017

    • Author(s)
      近江俊徳、中澤翔太、宇田川智野、多田尚美、三井寛子、落合和彦、 加藤由比子、呰上大吾、左向敏紀、筒井敏彦、盆子原誠、土田修一
    • Organizer
      第160回日本獣医学会学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] イヌ乳腺腫瘍細胞株で生じたp53遺伝子変異の検出と機能解析2017

    • Author(s)
      平間日奈子、落合和彦、道下正貴、近江俊徳
    • Organizer
      第48回日本比較臨床医学会学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] SGTA機能制御を起点とした 前立腺がん新規治療戦略の創出2016

    • Author(s)
      加藤由比子,落合和彦,近江俊徳
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2016-11-30
    • Related Report
      2016 Research-status Report
  • [Presentation] イヌ前立腺がん関連遺伝子SGTAがARシグナル伝達に与える影響の検討2016

    • Author(s)
      加藤由比子,落合和彦,近江俊徳
    • Organizer
      第159回日本獣医学会学術集会
    • Place of Presentation
      日本大学生物資源学部(藤沢)
    • Year and Date
      2016-09-06
    • Related Report
      2016 Research-status Report
  • [Presentation] 前立腺がん関連遺伝子SGTAイヌホモログの構造および機能解析2015

    • Author(s)
      加藤由比子。落合和彦。中平嶺、道下正貴、呰上大吾、高橋公正、近江俊徳
    • Organizer
      第158回日本獣医学会学術集会
    • Place of Presentation
      北里大学(青森)
    • Year and Date
      2015-09-07
    • Related Report
      2015 Research-status Report
  • [Presentation] イヌSGTAホモログのクローニングと前立腺がん細胞のアンドロゲンシグナル伝達機構に与える影響の検討2015

    • Author(s)
      落合和彦、加藤由比子、中平嶺、道下正貴、呰上大吾、高橋公正、近江俊徳
    • Organizer
      日本獣医臨床病理学会2015年大会
    • Place of Presentation
      日本獣医生命科学大学(東京)
    • Year and Date
      2015-05-30
    • Related Report
      2015 Research-status Report
  • [Remarks] 日本獣医生命科学大学獣医学部獣医保健看護学科TOP>>比較遺伝学研究分野

    • URL

      http://www.nvlu.ac.jp/veterinary-nursing/members/002.html/

    • Related Report
      2017 Annual Research Report

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Published: 2015-04-16   Modified: 2019-03-29  

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