| Project/Area Number |
15K10021
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Kanazawa University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
田村 昌也 金沢大学, 大学病院, 助教 (10397185)
高田 宗尚 金沢大学, 医学系, 協力研究員 (20459514)
加藤 寛城 金沢大学, 附属病院, 助教 (20733843)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 肺保存 / 不凍物質 / 微弱地場 / サブゼロ / 微弱磁場 |
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| Outline of Final Research Achievements |
The effectiveness of subzero lung preservation in a variable magnetic field using CAS with anti-freeze protein was examined.
We prepared a lung allograft model in rats. After reperfusion for 2 hours following 24-hour preservation, we evaluated the energy charge and reperfusion injury values of the transplanted lung in four groups. Group A: without preservation, Group B: at 4°C in a normal refrigerator, Group C: at -2°C in a CAS, Group D: 60 mg/ml of AFP to the preservation solution used for Group C. The outcome of Group C was better than that of Group B and equivalent to that of Group A and Group D. CAS was effective and adding AFP resulted in only a small added effectiveness. In addition, the effectiveness of anti-freeze proteins (AFP and AFGP) was examined in the same model as Group B. Anti-freeze proteins, in particular AFGP, are relatively effective. Furthermore, a separate experiment demonstrated that CAS with AFP was not significantly effective for -10°C lung cryopreservation.
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