Generation of transgenic Medaka producing a transporter targeting to fish oocytes

Research Project

Project/Area Number	15K14797
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Aquatic life science
Research Institution	Hokkaido University
Principal Investigator	HIRAMATSU Naoshi 北海道大学, 水産科学研究院, 准教授 (10443920)
Co-Investigator(Renkei-kenkyūsha)	IJIRI Shigeho 北海道大学, 大学院水産科学研究院, 准教授 (90425421) KAWAKAMI Koichi 国立遺伝学研究所, 教授 (70195048)
Project Period (FY)	2015-04-01 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000) Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords	分子輸送 / バイオリアクター / 卵 / ビテロジェニン / メダカ / 生物工学
Outline of Final Research Achievements	The aim of this study is to obtain the first technical basis for the development of a bio-reactor system through the production of model transporters in transgenic fish, with the final goal of which constructs a system transporting various effective materials across generations (i.e., from mother to egg or larva) in fish. Various expression constructs were generated by combining transporters, fluorescent proteins, a linker, trans-genic vector systems. Trans-genic medaka expressing fluorescent model transporters, as well as a model transporter with a linker, were produced as the second (F1) and the third (F2, the fluorescent transporter alone) trans-genic generations. This study provided fundamental knowledge for the next examination toward a massive bio-production of the transporters.