Identification of master genes for regulating developmental time in regenerated organs

• Project/Area Number: 15K15707
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Prosthodontics/ Dental materials science and
• Research Institution: Okayama University
• Principal Investigator: OSHIMA Masamitsu 岡山大学, 医歯薬学総合研究科, 助教 (00548307)
• Co-Investigator(Kenkyū-buntansha): 窪木 拓男 岡山大学, 医歯薬学総合研究科, 教授 (00225195) ; 大野 充昭 岡山大学, 医歯薬学総合研究科, 助教 (60613156) ; 秋山 謙太郎 岡山大学, 大学病院, 講師 (70423291)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 歯科補綴学一般 / 細胞生物学 / 発生・再生 / 歯

Outline of Final Research Achievements

We aimed to study the identification of master genes for regulating developmental time and morphogenesis in regenerated organs. We hypothesized that essential regulatory factors of developmental time and morphogenesis in tooth development could be identified by comparing the deciduous and permanent tooth germs harvested from an identical beagle dog. In this study, we identified the FGF14 and FEZF2 genes as candidates of the regulating factors by the cDNA microarray analysis. Furthermore, we analyzed the effects of these molecules on the murine tooth germ development. We have indicated that FGF14 promoted substantial elongation of the incisor tooth germ by acceleration of ameloblast proliferation. Further study to elucidate the molecular mechanism of FGF14 and FEZF2 on tooth development is necessary for the identification of the regulatory factors of developmental time and morphogenesis in tooth organogenesis.

Research Products

• [Journal Article] Practical whole-tooth restoration utilizing autologous bioengineered tooth germ transplantation in a postnatal canine model. (2017)
  • Author(s): Ono M, Oshima M, Ogawa M, Sonoyama W, Hara ES, Oida Y, Shinkawa S, Nakajima R , Mine A ,Hayano S, Fukumoto S , Kasugai8 S , Yamaguchi A, Tsuji T, Kuboki T
  • Journal Title: Sci Rep Volume: 7 Issue: 1 Pages: 44522-44522
  • DOI: 10.1038/srep44522
  • NAID: 120006535050
  • Peer Reviewed / Open Access
• [Journal Article] Challenges for stem cell-based "regenerative prosthodontics" (2017)
  • Author(s): Kunimichi Niibe, Fumio Suehiro, Masamitsu Oshima, Masahiro Nishimura, Takuo Kuboki, Hiroshi Egusa
  • Journal Title: Journal of Prosthodontic Research Volume: 61 Pages: 1-3
  • NAID: 130005465202
  • Peer Reviewed
• [Journal Article] Development of a functional biohybrid implant formed from periodontal tissue utilizing bioengineering technology. (2016)
  • Author(s): Nakajima K, Oshima M, Yamamoto N, Tanaka C, Koitabashi R, Inoue T, Tsuji T.
  • Journal Title: Tissue Engineering Part A. Volume: 22(17-18) Issue: 17-18 Pages: 1108-1115
  • DOI: 10.1089/ten.tea.2016.0130
  • Peer Reviewed / Open Access
• [Journal Article] Bioengineering a 3D integumentary organ system from iPS cells using an in vivo transplantation model (2016)
  • Author(s): 1Ryoji Takagi, Junko Ishimaru, Ayaka Sugawara, Koh-ei Toyoshima, Kentaro Ishida, Miho Ogawa, Kei Sakakibara, Kyosuke Asakawa, Akitoshi Kashiwakura, Masamitsu Oshima, Ryohei Minamide, Akio Sato, Toshihiro Yoshitake, Akira Takeda, Hiroshi Egusa & Takashi Tsuji
  • Journal Title: Science Advances Volume: 2(4) Issue: 4 Pages: 1-10
  • DOI: 10.1126/sciadv.1500887
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Hypothermic Temperature Effects on Organ Survival and Restoration. (2015)
  • Author(s): Ishikawa J, Oshima M, Iwasaki F, Suzuki R, Park J, Nakao K, Matsuzawa-Adachi Y, Mizutsuki T, Kobayashi A, Abe Y, Kobayashi E, Tezuka K, Tsuji T.
  • Journal Title: Scientific Reports Volume: 5 Issue: 1 Pages: 1-12
  • DOI: 10.1038/srep09563
  • Peer Reviewed / Open Access
• [Journal Article] A Novel Split Liver Protocol Using the Subnormothermic Oxygenated Circuit System in a Porcine Model of a Marginal Donor Procedure. (2015)
  • Author(s): Okada N, Mizuta K, Oshima M, Yamada N, Sanada Y, Ihara Y, Urahashi T, Ishikawa J, Tsuji T, Hishikawa S, Teratani T, Kobayashi E.
  • Journal Title: Transplantation Proceedings Volume: 47(2) Pages: 419-426
  • Peer Reviewed
• [Journal Article] Functional Tooth Restoration Utilising Split Germs Through Re-regionalisation of the Tooth-forming Field. (2015)
  • Author(s): Yamamoto N, Oshima M, Tanaka C, Ogawa M, Nakajima K, Ishida K, Moriyama K, Tsuji T.
  • Journal Title: Scientific Reports Volume: 5 Issue: 1 Pages: 1-10
  • DOI: 10.1038/srep18393
  • Peer Reviewed / Open Access
• [Presentation] 未来の歯科再生医療の実現に向けた器官再生技術の開発 (2017)
  • Author(s): 大島正充
  • Organizer: スタディグループ日曜会
  • Place of Presentation: 大阪、日本
  • Invited
• [Presentation] 歯周組織を有するバイオハイブリッドインプラントの開発 (2016)
  • Author(s): 大島正充、窪木拓男、辻孝
  • Organizer: 第36回公益社団法人日本口腔インプラント学会、中国・四国支部学術大会
  • Place of Presentation: 香川、日本
• [Presentation] 歯槽骨吸収に対する歯・歯周組織の包括的再生技術の開発 (2016)
  • Author(s): 大島正充、辻孝、窪木拓男
  • Organizer: 第7回骨バイオサイエンス研究会
  • Place of Presentation: 岡山、日本
• [Presentation] 歯胚分割技術を用いた歯の再生治療法の開発 (2015)
  • Author(s): 山本 直、大島正充、森山啓司、辻孝
  • Organizer: 第73回日本矯正歯科学会
  • Place of Presentation: 千葉,日本
  • Year and Date: 2015-10-21
• [Presentation] 歯科再生技術が導く歯・歯周組織の包括的再生 (2015)
  • Author(s): 大島正充
  • Organizer: 公益社団法人日本補綴歯科学会第124回学術大会
  • Place of Presentation: 埼玉,日本
  • Year and Date: 2015-05-30
• [Book] Horizontal Alveolar Ridge Augmentation in Implant Dentistry, A Surgical Manual. (2015)
  • Author(s): Oshima M, Tsuji T.
  • Total Pages: 340
  • Publisher: Wiley-Blackwell
• [Book] Engineering Mineralized and Load Bearing Tissues (2015)
  • Author(s): Oshima M, Tsuji T.
  • Total Pages: 276
  • Publisher: Springer International Publishing.
• [Remarks] 岡山大学プレスリリース
  • URL: https://www.okayama-u.ac.jp/tp/release/release_id457.html
• [Patent(Industrial Property Rights)] 歯科用インプラントデバイス (2016)
  • Inventor(s): 辻 孝、大島正充、窪木拓男、安藤嘉基、小川美帆
  • Industrial Property Rights Holder: 辻 孝、大島正充、窪木拓男、安藤嘉基、小川美帆
  • Industrial Property Rights Type: 特許
  • Filing Date: 2016-08-16