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Roles of Exosomal miRNA release from lung fibroblasts in the pathogenesis of COPD

Research Project

Project/Area Number 15K19413
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Respiratory organ internal medicine
Research InstitutionChiba University

Principal Investigator

Ikari Jun  千葉大学, 大学院医学研究院, 助教 (50734604)

Research Collaborator Tada Yuji  千葉大学, 大学院医学研究院, 講師 (50344990)
Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsエクソソーム / マイクロRNA / 肺線維芽細胞 / COPD / 肺繊維芽細胞
Outline of Final Research Achievements

In COPD, miR-146a is decreased in lung fibroblasts, leading to reduced repair function. Whether lung fibroblasts release exosomes contain miR-146a and whether this can be modulated by inflammatory cytokines is unclear. Human fetal lung fibroblasts (HFL-1) or primary adult control and COPD lung fibroblasts were cultured with and without IL-1β and TNF-α in vitro. HFL-1 cells secreted exosomes into cultured media both in the presence and absence of IL-1β and TNF-α. Cytokines stimulation significantly increased extracellular miR-146a secretion in a time and concentration dependent manner. Cytokines stimulated both exosomes secretion and intracellular miR-146a expression, leading to the augmented miR-146a release. COPD lung fibroblasts secreted less miR-146a into Exosomes than control lung fibroblasts. The current study demonstrates that lung fibroblasts release extracellular miR-146a, that this is modulated by cytokines and that COPD fibroblasts release less miR-146a.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (2 results)

All 2016 Other

All Int'l Joint Research (1 results) Presentation (1 results) (of which Int'l Joint Research: 1 results)

  • [Int'l Joint Research] Nebraska University Medical Center(米国)

    • Related Report
      2016 Annual Research Report
  • [Presentation] Extracellular Vesicles (EVs) from Normal Human Lung Fibroblasts Cells (NHLF) and COPD/Pulmonary Emphysema Cells Stimulated by IL-1β/TNF-α Mediate Divergent Effects2016

    • Author(s)
      Fumihiko Makino , Jun Ikari , Masae Makino , Amy Nelson , Hesham Basma , Xiangde Liu , Stephen I. Rennard
    • Organizer
      American Thoracic society conference 2016
    • Place of Presentation
      サンフランシスコ(米国)
    • Year and Date
      2016-05-19
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research

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Published: 2015-04-16   Modified: 2018-03-22  

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