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Feasibility Study for the generation of live cell from extinct or endangered animal

Research Project

Project/Area Number 16H02593
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Integrative animal science
Research InstitutionUniversity of Yamanashi

Principal Investigator

WAKAYAMA Teruhiko  山梨大学, 大学院総合研究部, 教授 (40360672)

Co-Investigator(Kenkyū-buntansha) 岸上 哲士  山梨大学, 大学院総合研究部, 教授 (10291064)
長友 啓明  山梨大学, 大学院総合研究部, 特任助教 (30746813)
大我 政敏  山梨大学, 大学院総合研究部, 特任助教 (40644886)
水谷 英二  東京大学, 医科学研究所, 特任研究員 (80443034)
Research Collaborator WAKAYAMA Sayaka  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥40,560,000 (Direct Cost: ¥31,200,000、Indirect Cost: ¥9,360,000)
Fiscal Year 2018: ¥12,870,000 (Direct Cost: ¥9,900,000、Indirect Cost: ¥2,970,000)
Fiscal Year 2017: ¥12,870,000 (Direct Cost: ¥9,900,000、Indirect Cost: ¥2,970,000)
Fiscal Year 2016: ¥14,820,000 (Direct Cost: ¥11,400,000、Indirect Cost: ¥3,420,000)
Keywordsクローン / 核移植 / 遺伝子資源 / 凍結乾燥 / 精子 / 絶滅危惧種 / 絶滅動物 / 顕微授精 / 卵子 / 核移植絶滅動物 / 染色体
Outline of Final Research Achievements

This study aims to recover and preserve genetic resources of extinct and endangered species. For this purpose, we tried to improve somatic cell nuclear cloning technology, and stability and tolerance of freeze-dried sperm nuclei were examined in order to conserve animal genetic resources. As a result, we succeeded in creating cloned mice from urine derived somatic cell nuclei. This result indicates that cloning techniques can increase the endangered species by non-invasively. We also discovered that freeze-dried spermatozoa can be stored at room temperature for over a year when it was preserved under high vacuum conditions.

Academic Significance and Societal Importance of the Research Achievements

本研究によってクローン技術の基礎研究が進み、動物を傷つけずにドナー核を入手できクローン個体を作り出せることが明らかとなった。これらの技術を応用すれば絶滅危惧種を安全に増やすことが可能になるだろう。一方遺伝子資源の保存方法として、精子の凍結乾燥の可能性を示すことができた。この方法なら宇宙ステーションで長期間マウスの精子を保存することも可能であり、地球規模の震災が起こっても、動物の遺伝子資源を確実に保存できる可能性を示すことができた。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (37 results)

All 2019 2018 2017 2016 Other

All Journal Article (16 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 16 results,  Open Access: 12 results,  Acknowledgement Compliant: 4 results) Presentation (18 results) (of which Int'l Joint Research: 8 results,  Invited: 8 results) Book (1 results) Remarks (2 results)

  • [Journal Article] Zygotic fluorescence recovery after photo-bleaching analysis for chromatin looseness that allows full-term development2018

    • Author(s)
      Ooga Masatoshi、Funaya Satoshi、Aoki Fugaku、Wakayama Teruhiko
    • Journal Title

      Journal of visualized experiments

      Volume: ー Issue: 136 Pages: 136-136

    • DOI

      10.3791/57068

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Assessing the tolerance to room temperature and viability of freeze-dried mice spermatozoa over long-term storage at room temperature under vacuum2018

    • Author(s)
      Kamada Yuko、Wakayama Sayaka、Shibasaki Ikue、Ito Daiyu、Kamimura Satoshi、Ooga Masatoshi、Wakayama Teruhiko
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 10602-10602

    • DOI

      10.1038/s41598-018-28896-8

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Single nucleolus precursor body formation in the pronucleus of mouse zygotes and SCNT embryos2018

    • Author(s)
      Kyogoku Hirohisa、Wakayama Teruhiko、Kitajima Tomoya S.、Miyano Takashi
    • Journal Title

      PLOS ONE

      Volume: 13 Issue: 8 Pages: e0202663-e0202663

    • DOI

      10.1371/journal.pone.0202663

    • NAID

      120006529672

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Generation of two-cell cloned embryos from mouse faecal cell2018

    • Author(s)
      Kamimura Satoshi、Wakayama Sayaka、Kuwayama Hiroki、Tanabe Yoshiaki、Kishigami Satoshi、Wakayama Teruhiko
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 14922-14922

    • DOI

      10.1038/s41598-018-33304-2

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Birth of cloned mice from vaginal smear cells after somatic cell nuclear transfer.2017

    • Author(s)
      Kuwayama H, Tanabe Y, Wakayama T, Kishigami S.
    • Journal Title

      Theriogenology.

      Volume: 94 Pages: 79-85

    • DOI

      10.1016/j.theriogenology.2017.02.012

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Healthy offspring from freeze-dried mouse spermatozoa held on the International Space Station for 9 months2017

    • Author(s)
      Sayaka Wakayama, Yuko Kamada, Kaori Yamanaka, Takashi Kohda, Hiromi Suzuki, Toru Shimazu,
    • Journal Title

      PNAS

      Volume: 1 Issue: 23 Pages: 1-6

    • DOI

      10.1073/pnas.1701425114

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] FRAP analysis of chromatin looseness in mouse zygotes that allows full-term development2017

    • Author(s)
      Ooga Masatoshi、Wakayama Teruhiko
    • Journal Title

      PLOS ONE

      Volume: 12 Issue: 5 Pages: e0178255-e0178255

    • DOI

      10.1371/journal.pone.0178255

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Reconstitution of the oocyte nucleolus in mice through a single nucleolar protein, NPM22017

    • Author(s)
      Ogushi Sugako、Yamagata Kazuo、Obuse Chikashi、Furuta Keiko、Wakayama Teruhiko、Matzuk Martin M.、Saitou Mitinori
    • Journal Title

      J Cell Sci.

      Volume: 130 Issue: 14 Pages: 2416-2429

    • DOI

      10.1242/jcs.195875

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Derivation of ground-state female ES cells maintaining gamete-derived DNA methylation2017

    • Author(s)
      Yagi M, Kishigami S, Tanaka A, Semi K, Mizutani E, Wakayama S, Wakayama T, Yamamoto T, Yamada Y.
    • Journal Title

      Nature

      Volume: 548 Issue: 7666 Pages: 224-227

    • DOI

      10.1038/nature23286

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Production of cloned mice using oocytes derived from ICR-outbred strain.2017

    • Author(s)
      Tanabe Y, Kuwayama H, Wakayama S, Nagatomo H, Ooga M, Kamimura S, Kishigami S, Wakayama T.
    • Journal Title

      Reproduction

      Volume: 154(6) Issue: 6 Pages: 859-866

    • DOI

      10.1530/rep-17-0372

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Agarose capsules as new tools for protecting denuded mouse oocytes/embryos during handling and freezing-thawing and supporting embryonic development in vivo.2017

    • Author(s)
      Nagatomo H, Yao T, Araki Y, Mizutani E, Wakayama T.
    • Journal Title

      Scientific reports

      Volume: 7 Issue: 1 Pages: 17960-17960

    • DOI

      10.1038/s41598-017-18365-z

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The Number of Point Mutations In iPS Cells and ntES Cells Depends on the Method and Somatic Cell Type Employed for Their Generation.2017

    • Author(s)
      Araki R, Mizutani E, Hoki Y, Sunayama M, Wakayama S, Nagatomo H, Kasama Y, Nakamura M, Wakayama T, Abe M.
    • Journal Title

      Stem Cells

      Volume: 35 Issue: 5 Pages: 1189-1196

    • DOI

      10.1002/stem.2601

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Generation of cloned mice and nuclear transfer embryonic stem cell lines from urine-derived cells.2016

    • Author(s)
      Mizutani E, Torikai K, Wakayama S, Nagatomo H, Ohinata Y, Kishigami S, Wakayama T.
    • Journal Title

      Scientific Reports.

      Volume: 6 Issue: 1 Pages: 23808-23808

    • DOI

      10.1038/srep23808

    • NAID

      130007023594

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Phenotypes of Aging Postovulatory Oocytes After Somatic Cell Nuclear Transfer in Mice.2016

    • Author(s)
      Lee AR, Shimoike T, Wakayama T, Kishigami S.
    • Journal Title

      Cell Reprogram

      Volume: 18 Issue: 3 Pages: 147-153

    • DOI

      10.1089/cell.2016.0014

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of Long-Term Exposure of Donor Nuclei to the Oocyte Cytoplasm on Production of Cloned Mice Using Serial Nuclear Transfer.2016

    • Author(s)
      Wakayama S, Tanabe Y, Nagatomo H, Mizutani E, Kishigami S, Wakayama T.
    • Journal Title

      Cell Reprogram

      Volume: 18 Issue: 6 Pages: 382-389

    • DOI

      10.1089/cell.2016.0026

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Aberrant Expression of TIMP-2 and PBEF Genes in the Placentae of Cloned Mice Due to Epigenetic Reprogramming Error.2016

    • Author(s)
      Kim HR, Lee JE, Oqani RK, Kim SY, Wakayama T, Li C, Sa SJ, Woo JS, Jin DI.
    • Journal Title

      PLoS One.

      Volume: 11 Issue: 11 Pages: e0166241-e0166241

    • DOI

      10.1371/journal.pone.0166241

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Animal cloning from extinct or endangered species by nuclear injection2018

    • Author(s)
      Teruhiko Wakayama
    • Organizer
      International conference 2018 BME Session: CELL REPROGRAMMING AND REPRODUCTIVE BIOTECHNOLOGY
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Freeze-dried sperm preserved on space station.2018

    • Author(s)
      Teruhiko Wakayama
    • Organizer
      Environmental impact on reproduction/fertility
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 哺乳類精子の室温保存を目指して2018

    • Author(s)
      若山照彦
    • Organizer
      Cryopreservation Conference 2018
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Production of cloned mice using oocytes derived from ICR strain.2017

    • Author(s)
      Yoshiaki Tanabe, Hiroki Kuwayama, Sayaka Wakayama, Hiroaki Nagatomo, Masatoshi Ooga, Satoshi Kamimura, Satoshi Kishigami, and Teruhiko Wakayama
    • Organizer
      4th World Congress of Reproductive Biology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Unique method for producing oocyte that decreased spindle formation factors2017

    • Author(s)
      Shunsuke Konno, Masatoshi Ooga, Satoshi Kamimura, Sayaka Wakayama and Teruhiko Wakayama
    • Organizer
      4th World Congress of Reproductive Biology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Improvement of freeze-drying preservation method of spermatozoa at room temperature.2017

    • Author(s)
      Yuko Kamada, Daiyu Ito, Ikue Shibasaki, Satoshi Kamimura, Hiroaki Nagatomo, Masatoshi Ooga, Sayaka Wakayama and Teruhiko Wakayama
    • Organizer
      4th World Congress of Reproductive Biology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Developmental competence of mouse oocytes activated by injection of PLCζ from different species2017

    • Author(s)
      Yunosuke Yamamoto, Masatoshi Ooga, Satoshi Kamimura, Hiroaki Nagatomo, Sayaka Wakayama, Junya Ito, and Teruhiko Wakayama
    • Organizer
      4th World Congress of Reproductive Biology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The success of the pronuclear formation in mouse somatic cell nuclear transfer using fecal cells.2017

    • Author(s)
      Satoshi Kamimura, Sayaka Wakayama, Hiroki Kuwayama, Yoshiaki Tanabe, Satoshi Kishigami, and Teruhiko Wakayama
    • Organizer
      4th World Congress of Reproductive Biology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Disrupted parental asymmetry of chromatin structure in ROSI-derived zygotes.2017

    • Author(s)
      Masatoshi Ooga, and Teruhiko Wakayama
    • Organizer
      4th World Congress of Reproductive Biology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 絶滅動物の復活を目指して2016

    • Author(s)
      若山照彦
    • Organizer
      第34回日本受精着床学会
    • Place of Presentation
      軽井沢プリンスホテルウエスト、長野県
    • Year and Date
      2016-09-30
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] フリーズドライ精子の常温長期保存の試み2016

    • Author(s)
      鎌田 裕子、若山 清香、若山照彦
    • Organizer
      109回日本繁殖生物学会
    • Place of Presentation
      麻布大学 神奈川県
    • Year and Date
      2016-09-12
    • Related Report
      2016 Annual Research Report
  • [Presentation] マウス2細胞期胚において分配異常を起こした染色体の回収と同定の試み2016

    • Author(s)
      柴﨑郁江、鎌田裕子、鳥飼昂平、長友啓明、水谷英二、若山照彦
    • Organizer
      109回日本繁殖生物学会
    • Place of Presentation
      麻布大学 神奈川県
    • Year and Date
      2016-09-12
    • Related Report
      2016 Annual Research Report
  • [Presentation] ICR卵子を用いたクローンマウスの作出について2016

    • Author(s)
      田邊圭啓、桑山拡樹、 岸上哲士、若山照彦
    • Organizer
      109回日本繁殖生物学会
    • Place of Presentation
      麻布大学 神奈川県
    • Year and Date
      2016-09-12
    • Related Report
      2016 Annual Research Report
  • [Presentation] 膣垢細胞を用いたクローンマウス産仔の作出と検討2016

    • Author(s)
      桑山拡樹、田邊圭啓、若山照彦、岸上哲士
    • Organizer
      109回日本繁殖生物学会
    • Place of Presentation
      麻布大学 神奈川県
    • Year and Date
      2016-09-12
    • Related Report
      2016 Annual Research Report
  • [Presentation] 生殖バイオテクノロジーの最前線 ~クローン技術から宇宙での生殖まで~2016

    • Author(s)
      若山照彦
    • Organizer
      第30回関東臨床細胞学会
    • Place of Presentation
      山梨県立文学館、山梨県
    • Year and Date
      2016-09-10
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 子孫の新しい作り方 ―クローンや宇宙繁殖などSFっぽい新技術―2016

    • Author(s)
      若山照彦
    • Organizer
      第9回生殖細胞の会
    • Place of Presentation
      東京海洋大学水圏科学フィールド教育研究センター、 山梨県
    • Year and Date
      2016-07-30
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] クローン技術の過去・現在・未来2016

    • Author(s)
      若山照彦
    • Organizer
      第15回生殖バイオロジー東京シンポジウム
    • Place of Presentation
      都市センターホテル、 東京都
    • Year and Date
      2016-06-17
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Production of offspring from cells derived from carcass or cast off material2016

    • Author(s)
      Teruhiko Wakayama
    • Organizer
      Epigenetic Penetrance of Reproductive Technologies
    • Place of Presentation
      University of Teramo, Teramo, Italy
    • Year and Date
      2016-06-09
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Book] Methods Mol Biol. Micro-injection2019

    • Author(s)
      Wakayama S, Kishigami S, Wakayama T.
    • Total Pages
      540
    • Publisher
      Humana Press
    • ISBN
      9781493988303
    • Related Report
      2018 Annual Research Report
  • [Remarks] 山梨大学 発生工学研究センター

    • URL

      http://www.ccn.yamanashi.ac.jp/~twakayama/LSHP/index.html

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report 2016 Annual Research Report
  • [Remarks] 若山研究室 山梨大学生命環境学部生命工学科

    • URL

      http://www.ccn.yamanashi.ac.jp/~twakayama/LSHP/Wakayama%20lab/index.html

    • Related Report
      2016 Annual Research Report

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Published: 2016-04-21   Modified: 2020-03-30  

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