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Deciphering the mechanism of acquisition and evolution of symbiotic microbes in the termite gut

Research Project

Project/Area Number 16H04840
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Ecology/Environment
Research InstitutionTokyo Institute of Technology

Principal Investigator

Hongoh Yuichi  東京工業大学, 生命理工学院, 教授 (90392117)

Co-Investigator(Kenkyū-buntansha) 山田 明徳  長崎大学, 水産・環境科学総合研究科(水産), 准教授 (40378774)
伊藤 武彦  東京工業大学, 生命理工学院, 教授 (90501106)
Research Collaborator Igai Katsura  東京工業大学, 生命理工学院, 研究員
Morikawa Takahiro  東京工業大学, 生命理工学院, 大学院生
Takahashi Yudai  東京工業大学, 生命理工学院, 大学院生
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2018: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2017: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2016: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Keywordsシロアリ / 共生 / 腸内微生物 / 原生生物 / セルロース / 進化 / 昆虫 / 腸内微生物群集 / セルラーゼ / 木質分解
Outline of Final Research Achievements

This study aimed to decipher how termites have acquired their gut microbes that are responsible for digesting dead plant matter (lignocellulose). For this purpose, I focused on one clade of higher termites that has relatively recently re-acquired protists as a dominant component of the gut microbiota after the loss of the original cellulolytic protist assemblages. As a result, my research team revealed that the novel protists are ubiquitous in diverse termite lineages but have become dominant only in the specific termite clade. In addition, we found that the protist cytoplasm is filled with wood particles, and the gut compartment containing the protists showed significant cellulolytic activities. Thus, in this study, we obtained fundamental information on this new group of protists for future studies.

Academic Significance and Societal Importance of the Research Achievements

本研究では、比較的最近、新規に獲得されたと見られるシロアリ腸内微生物を題材とし、その系統と多様性、セルロース分解活性などを明らかにした。シロアリは木材の大害虫であるとともに、自然界では重要な分解者であり、その生理・生態の解明は基礎・応用科学の両面で重要である。本研究により、シロアリが腸内共生微生物を獲得する進化過程の一部が解明され、今後のシロアリ研究の基盤となる情報を得ることができた。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (29 results)

All 2019 2018 2017 2016 Other

All Int'l Joint Research (7 results) Journal Article (8 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 6 results,  Open Access: 4 results,  Acknowledgement Compliant: 1 results) Presentation (13 results) (of which Int'l Joint Research: 6 results,  Invited: 8 results) Remarks (1 results)

  • [Int'l Joint Research] University of Sydney(オーストラリア)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Kasetsart University/Valaya Alongkorn Rajabhat University(タイ)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Kasetsart University/Valaya Alongkorn Rajabhat University(タイ)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] University of Sydney(オーストラリア)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Kasetsart University(タイ)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Suranaree University of Technology(タイ)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] University of Sydney(オーストラリア)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] 材食を可能とするシロアリ腸内多重共生機構の解明2019

    • Author(s)
      本郷裕一
    • Journal Title

      昆虫と自然(ニューサイエンス社)

      Volume: 54 Pages: 35-38

    • NAID

      40022290331

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Ectosymbiotic bacterial microbiota densely colonize the surface of thelastomatid nematodes in the gut of the wood-feeding cockroach Panesthia angustipennis2018

    • Author(s)
      Murakami Takumi、Onouchi Shu、Igai Katsura、Ohkuma Moriya、Hongoh Yuichi
    • Journal Title

      FEMS Microbiology Ecology

      Volume: 95 Issue: 2

    • DOI

      10.1093/femsec/fiy238

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genome analyses of uncultured TG2/ZB3 bacteria in ‘Margulisbacteria’ specifically attached to ectosymbiotic spirochetes of protists in the termite gut2018

    • Author(s)
      Utami Yuniar Devi、Kuwahara Hirokazu、Igai Katsura、Murakami Takumi、Sugaya Kaito、Morikawa Takahiro、Nagura Yuichi、Yuki Masahiro、Deevong Pinsurang、Inoue Tetsushi、Kihara Kumiko、Lo Nathan、Yamada Akinori、Ohkuma Moriya、Hongoh Yuichi
    • Journal Title

      The ISME Journal

      Volume: 13 Issue: 2 Pages: 455-467

    • DOI

      10.1038/s41396-018-0297-4

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Metagenomic analyses highlight the symbiotic association between the glacier stonefly Andiperla willinki and its bacterial gut community2018

    • Author(s)
      Murakami, T., Segawam T., Takeuchi, N., Barcaza, G., Lavarcam P., Kohshima, S., Hongoh, Y.
    • Journal Title

      Environmental Microbiology

      Volume: 20 Issue: 11 Pages: 4170-4183

    • DOI

      10.1111/1462-2920.14420

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] ゲノム解析によるシロアリ腸内原生生物共生細菌の役割の解明2018

    • Author(s)
      本郷裕一
    • Journal Title

      しろあり(公益社団法人日本しろあり対策協会 機関誌)

      Volume: 169 Pages: 12-26

    • NAID

      40021458992

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Phylogenetic diversity and single-cell genome analysis of "Melainabacteria", a non-photosynthetic cyanobacterial group, in the termite gut2018

    • Author(s)
      Utami Y.D., Kuwahara H., Murakami T., Morikawa T., Sugaya K., Kihara K., Yuki M., Lo N., Deevong P., Hasin S., Boonriam W., Inoue T., Yamada A., Ohkuma M., Hongoh Y.
    • Journal Title

      Microbes and Environments

      Volume: 33 Pages: 50-57

    • NAID

      130006581744

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Discovery and Complete Genome Sequence of a Bacteriophage from an Obligate Intracellular Symbiont of a Cellulolytic Protist in the Termite Gut2017

    • Author(s)
      Pramono A.K., Kuwahara H., Itoh T., Toyoda A., Yamada A., Hongoh Y
    • Journal Title

      Microbes and Environments

      Volume: 32 Issue: 2 Pages: 112-117

    • DOI

      10.1264/jsme2.ME16175

    • NAID

      130006789871

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Discovery of ectosymbiotic Endomicrobium lineages associated with protists in the gut of stolotermitid termites2017

    • Author(s)
      Izawa Kazuki、Kuwahara Hirokazu、Sugaya Kaito、Lo Nathan、Ohkuma Moriya、Hongoh Yuichi
    • Journal Title

      Environmental Microbiology Reports

      Volume: 9 Issue: 4 Pages: 411-418

    • DOI

      10.1111/1758-2229.12549

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Genomics of uncultivable bacteria deciphers the multilayered symbiotic system in the termite gut2018

    • Author(s)
      Yuichi Hongoh
    • Organizer
      OIST seminar
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Multilayered-symbiotic system in the termite gut2018

    • Author(s)
      Yuichi Hongoh
    • Organizer
      Central China Normal University Seminar
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Genomics of uncultivable bacteria deciphers multilayered symbiotic system in the termite gut2018

    • Author(s)
      Yuichi Hongoh
    • Organizer
      2018 World Life Science Conference (Beijing, China)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Multi-layered symbiotic system in the termite gut2018

    • Author(s)
      Yuichi Hongoh
    • Organizer
      第46回内藤カンファレンス
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Genomics of uncultivable bacteria deciphers multilayered symbiotic system in the termite gut2018

    • Author(s)
      Yuichi Hongoh
    • Organizer
      International Workshop on Insect Gut Microbiology, Max Planck Institute(Marburg)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Single-cell genomics deciphers the termite gut ecosystem2018

    • Author(s)
      Yuichi Hongoh
    • Organizer
      第91回日本細菌学会総会 国際シンポジウム「Frontiers in Bacterial Genomics
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 高等シロアリ腸内共生原生生物の多様性と木質分解への寄与2017

    • Author(s)
      守川貴裕、猪飼桂、高橋雄大、金井英樹、大熊盛也、井上徹志、山田明徳、本郷裕一
    • Organizer
      第61回日本応用動物昆虫学会大会
    • Place of Presentation
      農工大、東京
    • Year and Date
      2017-03-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] 1細胞ゲノムとメタゲノムから読み解くシロアリ腸内多重共生系2017

    • Author(s)
      本郷裕一
    • Organizer
      平成28年度EIIRISプロジェクト研究成果報告会
    • Place of Presentation
      豊橋技大、豊橋
    • Year and Date
      2017-03-10
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 高等シロアリ及びゴキブリ腸内共生原生生物の系統的多様性とその形態2017

    • Author(s)
      髙橋雄大,猪飼桂,守川貴裕,菅谷快斗,大熊盛也,小林正規,井上徹志,山田明徳,本郷裕一
    • Organizer
      第1回日本共生生物学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 高等シロアリ腸内木質分解原生生物の再獲得の可能性2017

    • Author(s)
      猪飼桂,守川貴裕,髙橋雄大,守屋繁春, 大熊盛也,井上徹志,山田明徳,本郷裕一
    • Organizer
      第1回日本共生生物学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] シロアリはなぜ木だけを食べて生きられるのか2017

    • Author(s)
      本郷裕一
    • Organizer
      名古屋大学セミナー
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] シロアリ腸内多重共生系の進化2016

    • Author(s)
      本郷裕一
    • Organizer
      日本進化学会第18回大会
    • Place of Presentation
      東工大、東京
    • Year and Date
      2016-08-26
    • Related Report
      2016 Annual Research Report
  • [Presentation] シロアリと絶対共生する腸内微生物群集の進化と機能2016

    • Author(s)
      本郷裕一
    • Organizer
      第27回日本生体防御学会総会
    • Place of Presentation
      九大、福岡
    • Year and Date
      2016-07-08
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Remarks] 本郷研ホームページ

    • URL

      http://www.hongoh.bio.titech.ac.jp

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report

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Published: 2016-04-21   Modified: 2020-03-30  

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