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Analysis of maturing of crops in relation to plant water

Research Project

Project/Area Number 16H04867
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Crop production science
Research InstitutionKyushu University

Principal Investigator

Iwaya-Inoue Mari  九州大学, 農学研究院, 学術特任教員 (2016年度 教授) (60091394)

Co-Investigator(Kenkyū-buntansha) 湯淺 高志  宮崎大学, 農学部, 教授 (40312269)
石橋 勇志  九州大学, 農学研究院, 准教授 (50611571)
鄭 紹輝  佐賀大学, 農学部, 教授 (90253517)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2016: ¥13,000,000 (Direct Cost: ¥10,000,000、Indirect Cost: ¥3,000,000)
Keywords乾燥ストレス / ダイズ / ササゲ / 子実 / 一斉登熟 / 貯蔵物質 / 青立ち / 登熟 / 養分動態 / 水の動態 / 作物生産 / 植物ホルモン / 莢先熟 / 水分生理 / 作物学 / シンクソースバランス
Outline of Final Research Achievements

Unlike other annual legume crops such as cowpea, soybean leaves drop, and stems lose their green color with seed maturation. A soybean-specific phenomenon called green stem syndrome (GSS) prevents soybean stems and leaves from drying down properly, albeit the mature seeds lose water. GSS lowers both grain yield and grain quality and interferes with machine harvesting. Our results suggested that cytokinin and autophagy were involved in GSS of soybean. In addition, drought stress caused decrease in seed dry weight due to decrease in contents of protein and lipids while increase in soluble sugar content in seeds.
On the other hand, drought stress in cowpea did not affect germination rate, indicating that seed viability and its quality were maintained. Consequently, cowpea yield under drought stress was maintained, despite a decrease in photosynthetic rate, by translocation of photoassimilates from leaves via temporary sink and source organs, especially from flower stalks to the seeds.

Academic Significance and Societal Importance of the Research Achievements

ダイズは他のマメ科作物と異なり一斉登熟性をもつが、「青立ち」株では機械収穫の際に茎葉の水分により汚粒を発生し、古くから農業上の大きな問題となってきた。本研究では、一斉登熟性をもたないササゲと比較し、摘莢や乾燥ストレスを与え、シンクソースバランスを崩すことにより「青立ち」のメカニズムを解明し、栽培技術の向上を目指した。
根や葉の植物ホルモンに着目した結果、ダイズでは、サイトカイニンの制御が青立ち抑制に有効であることを明らかにした。一方、ササゲでは、乾燥ストレスによりABA合成が促進され、葉の脱水に伴い、糖は花柄へ移動し、さらに花柄から子実への転流促進により、登熟が正常に起ることを示した。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (29 results)

All 2019 2018 2017 2016 Other

All Journal Article (7 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 7 results,  Open Access: 4 results,  Acknowledgement Compliant: 1 results) Presentation (18 results) (of which Int'l Joint Research: 5 results,  Invited: 1 results) Book (2 results) Remarks (2 results)

  • [Journal Article] Variation in photosensitivity of flowering in the world soybean mini-core collections (GmWMC).2019

    • Author(s)
      Islam, M. R. , D. Fujita, S. Watanabe and S. H. Zheng
    • Journal Title

      Plant Production Sci.

      Volume: - Issue: 2 Pages: 1-7

    • DOI

      10.1080/1343943x.2018.1561197

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Drought stress during soybean seed filling affects storage compounds through regulation of lipid and protein metabolism2018

    • Author(s)
      Nakagawa, A. C. S., H. Itoyama, Y. Ariyoshi, N. Ario, Y. Tomita, Y. Kondo, M. Iwaya-Inoue and Y. Ishibashi
    • Journal Title

      Acta Physiologiae Plantarum

      Volume: 40 Issue: 6 Pages: 111-111

    • DOI

      10.1007/s11738-018-2683-y

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Vegetative growth after flowering through gibberellin biosynthesis regulates pod setting rate in soybean (Glycine max (L.) Merr.)2018

    • Author(s)
      Taniguchi, T., N. Murayama, M. Hasegawa, A.C.S. Nakagawa, S. Tanaka, S.-H.Zheng, N. Hamaoka, M. Iwaya-Inoue and Y. Ishibashi
    • Journal Title

      Plant Signaling & Behavior

      Volume: 13 Pages: 1-5

    • DOI

      10.1080/15592324.2018.1473668

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Seed dormancy induced by extreme dehydration and its breaking mechanism in relation to water status and reactive oxygen species2018

    • Author(s)
      Ishibashi, Y., T. Yuasa and M. Iwaya-Inoue
    • Journal Title

      Advances in Experimental Medicine and Biology, Springer, Singapore

      Volume: 1081 Pages: 233-257

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 植物の低温・乾燥耐性における水分生理学的研究2018

    • Author(s)
      井上眞理
    • Journal Title

      低温生物工学会

      Volume: 64 Pages: 1-11

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Nitrogen manipulation affects leaf senescence during late seed filling in soybean2017

    • Author(s)
      Md. Matiul Islam, Yushi Ishibashi, Andressa C. S. Nakagawa, Yuki Tomita, Xin Zhao, Mari Iwaya-Inoue, Susumu Arima, Shao-Hui Zheng
    • Journal Title

      Acta Physiologiae Plantarum

      Volume: 39 Issue: 1 Pages: 42-42

    • DOI

      10.1007/s11738-016-2334-0

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Effects of light quality on pod elongation in soybean (Glycine max (L.) Merr.) and cowpea (Vigna unguiculata (L.) Walp.).2017

    • Author(s)
      Tanaka S, Ario N, Nakagawa ACS, Tomita Y, Murayama N, Taniguchi T, Hamaoka N, Iwaya-Inoue M, Ishibashi Y.
    • Journal Title

      Plant Signal Behav.

      Volume: 12 Issue: 6 Pages: 6-6

    • DOI

      10.1080/15592324.2017.1327495

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] ダイズ(Glycine max (L.) Merr.)における莢成長関連遺伝子GmPSS9の多型による転写制御機構の解明2019

    • Author(s)
      田中征矢, A. C.S. Nakagawa, 谷口琢紀, 堀川駿, 小西拓哉, 黒瀬良太, 村山直暉, 長谷川光夫, 濱岡範光, 井上眞理, 石橋勇志
    • Organizer
      日本作物学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ダイズの日長による結莢制御機構に関する基礎的研究 日本作物学会講演会要旨集 2019年 247 巻 2019/03/262019

    • Author(s)
      谷口琢紀, 村山直暉, 長谷川光夫, Anderessa C.S. Nakagawa, 田中征矢, 小西拓哉, 堀川駿, 黒瀬良太, 富田雄貴, 濱岡範光, 井上眞理, 石橋勇志
    • Organizer
      日本作物学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ササゲの乾燥耐性品種における深根性形質の検討2018

    • Author(s)
      辰己由華,村上真哉, 石橋勇志, 井上眞理
    • Organizer
      低温生物工学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ダイズの老化・栄養飢餓に応答したアミノ酸代謝とオートファジー調節メカニズム2018

    • Author(s)
      川口颯介, 瀧石英雄, 石橋孝明, 湯淺高志
    • Organizer
      三学会合同宮崎大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] マメ科作物の環境ストレス・栄養シグナルに応答した オートファジー誘導とアミノ酸代謝シグナル2018

    • Author(s)
      藤巻航, 川口颯介, Do Tuan Tung, Yee Yee Tun, 瀧石英雄, 石橋孝明, Naroon Waramit, Sontichai Chanprame, Ratchanee Hongprayoon,湯淺高志
    • Organizer
      好塩微生物研究会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ササゲのストレスメモリーを介した乾燥地適応戦略2018

    • Author(s)
      三浦寧音, 辰己由華, 濱岡範光, 井上眞理, 石橋勇志
    • Organizer
      日本作物学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 乾燥および摘莢処理がダイズの子実成分に及ぼす影響 (Influence of source and sink manipulation on soybean seed storage metabolism)2018

    • Author(s)
      中川 アンドレッサ, 田中征矢, 村山直暉, 谷口琢紀, 長谷川光夫, 濱岡範光, 井上眞理, 石橋勇志
    • Organizer
      日本作物学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ダイズの莢先熟発生機構の解析;地下部におけるサイトカイニン合成の影響について2017

    • Author(s)
      富田雄貴、中川カミラ聖子アンドレッサ、田中征矢、村山直暉、谷口琢紀、濱岡範光、石橋勇志、井上眞理
    • Organizer
      第243回日本作物学会
    • Place of Presentation
      東京大学、東京都
    • Year and Date
      2017-03-29
    • Related Report
      2016 Annual Research Report
  • [Presentation] ダイズの莢先熟発生機構の解析;窒素とサイトカイニンの関係について2017

    • Author(s)
      富田雄貴、中川カミラ聖子アンドレッサ、田中征矢、村山直暉、谷口琢紀、濱岡範光、石橋勇志、井上眞理
    • Organizer
      第243回日本作物学会
    • Place of Presentation
      東京大学、東京都
    • Year and Date
      2017-03-29
    • Related Report
      2016 Annual Research Report
  • [Presentation] 植物の低温・乾燥耐性における水分生理学的研究2017

    • Author(s)
      井上眞理
    • Organizer
      低温生物工学会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Identification of a candidate gene for pod growth in soybean (Glycine max (L.) Merr.)2017

    • Author(s)
      Tanaka, S., Y. Ariyoshi, ACS Nakagawa, Y. Tomita, N. Murayama, T. Taniguchi, M.Hasegawa, N. Hamaoka N, M. Iwaya-Inoue and Y. Ishibashi
    • Organizer
      AFELiSA 2017 (The 14th International Joint Symposium among Japan and Korea)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effects of drought stress on water-related parameters and photoassimilate translocation in cowpea (Vigna unguiculata (L.) Walp) at pod-filling stage2017

    • Author(s)
      Egashira, C., N. Miura, T. Yamauchi, Y. Miyamoto, Y. Hashiguchi, Y. Matsushima, T. Yuasa, Y. Ishibashi and M. Iwaya-Inoue
    • Organizer
      AFELiSA 2017 (The 14th International Joint Symposium among Japan and Korea)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Mechanism of drought stress memory in cowpea and soybean plants2017

    • Author(s)
      Miura N., S. Murakami, M. Tochihara, R. Komai, N. Hamaoka, Y. Ishibashi and M. Iwaya-Inoue
    • Organizer
      AFELiSA 2017 (The 14th International Joint Symposium among Japan and Korea)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ササゲの乾燥適応におけるVuWRKYの役割2017

    • Author(s)
      栃原美咲, 湯淺高志,三浦寧音, 駒井玲花, 辰巳由華, 濱岡範光, 石橋勇志, 井上眞理
    • Organizer
      低温生物工学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Increasing of endogenous cytokinin in reproductive stage is involved in green stem syndrome of soybean2016

    • Author(s)
      1.Tomita Y., Andressa C. S. Nakagawa, S. Tanaka, N. Murayama, T.Taniguchi, N. Hamaoka, Y. Ishibashi, and M. Iwaya-Inoue
    • Organizer
      AFELiSA 2016 (The13th International Joint Symposium among Japan and Korea)
    • Place of Presentation
      Daejeon, Korea
    • Year and Date
      2016-11-09
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Mehanism of drought stress memory in cowpea and soybean plants2016

    • Author(s)
      Miura N., S. Murakami, M. Tochihara, R. Komai, N. Hamaoka, Y. Ishibashi, M. Iwaya-Inoue
    • Organizer
      AFELiSA 2016 (The13th International Joint Symposium among Japan and Korea)
    • Place of Presentation
      Daejeon, Korea
    • Year and Date
      2016-11-09
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ササゲとダイズにおけるABAを介した乾燥ストレスメモリー機構2016

    • Author(s)
      三浦寧音, 村上真哉, 栃原美咲, 駒井玲花, 濱岡範光, 井上眞理, 石橋勇志
    • Organizer
      第120回日本熱帯作物学会
    • Place of Presentation
      鹿児島大学、鹿児島市
    • Year and Date
      2016-10-08
    • Related Report
      2016 Annual Research Report
  • [Presentation] ササゲとダイズのABAを介した乾燥応答メカニズムの解明2016

    • Author(s)
      栃原美咲, 村上真哉, 石橋勇志, 井上眞理
    • Organizer
      第240回日本作物学会
    • Place of Presentation
      信州大学、長野市
    • Year and Date
      2016-09-05
    • Related Report
      2016 Annual Research Report
  • [Book] Survival Strategies in Extreme Cold and Desiccation: Adaptation Mechanisms and their Application. Advances in Experimental Medicine and Biology 10812018

    • Author(s)
      Iwaya-Inoue, M., M. Sakurai, M. Uemura (Eds)
    • Total Pages
      409
    • Publisher
      Springer, Singapore
    • ISBN
      9789811312441
    • Related Report
      2018 Annual Research Report
  • [Book] Mechanisms of Maturation and Germination in Crop Seeds Exposed to Environmental Stresses with a Focus on Nutrients, Water Status, and Reactive Oxygen Species. In: Survival Strategies in Extreme Cold and Desiccation: Adaptation Mechanisms and their Applications, Series of Advances in Experimental Medicine and Biology. (Eds) Iwaya-Inoue M, Sakurai M, Uemura M2018

    • Author(s)
      Ishibashi, Y., T. Yuasa and M. Iwaya-Inoue
    • Publisher
      Springer
    • Related Report
      2017 Annual Research Report
  • [Remarks]

    • URL

      http://www.agr.kyushu-u.ac.jp/lab/sakumotsu/

    • Related Report
      2018 Annual Research Report
  • [Remarks] 九州大学 作物学研究室

    • URL

      http://www.agr.kyushu-u.ac.jp/lab/sakumotsu/

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report

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Published: 2016-04-21   Modified: 2021-02-19  

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