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Study on maternal antibody transfer in avian species utilizing IgY-deficient chickens

Research Project

Project/Area Number 16H05020
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Animal production science
Research InstitutionNagoya University

Principal Investigator

Murai Atsushi  名古屋大学, 生命農学研究科, 准教授 (10313975)

Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2019: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2018: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Keywordsニワトリ / ウズラ / 家禽 / 卵黄抗体 / IgY / IgY欠損鶏 / 受容体 / 母子免疫 / 畜産学 / IgY抗体 / 卵黄 / 候補受容体 / 生理学 / 栄養学 / IgY欠損 / 卵母細胞 / 抗体 / 栄養生理学 / ファブリキウス嚢 / 胚盤 / マイクロアレイ
Outline of Final Research Achievements

Blood IgY is incorporated into egg yolks of laying birds. The maternal IgY is essential for enhancing immunity of the new-born chicks. Uptake of blood IgY into egg yolks relies on the receptor present in the ovarian follicle, but there is no report to identify the receptor. In the present study, we tried to find out candidate receptor by comparing gene expression levels in oocytes of IgY-deficient or normal chickens. Two receptor candidate genes, LRP2L and FcRY, were selected from the genes in which the gene expression level was increased in IgY-deficient chickens compared to the control chicken. Synthesized recombinant FcRY bound to IgY, whereas recombinant LRP2L did not bind to IgY. The present study showed that FcRY expressed in ovarian follicles is involved in IgY transport into egg yolks in avian maternal immunity.

Academic Significance and Societal Importance of the Research Achievements

母体の免疫成分が新生仔に継承される現象は母子免疫と呼ばれ、鳥類では、母ドリの血中抗体IgYが大量に卵黄へ取り込まれる。この現象は100年以上も前から知られているが、その分子機構は今日まで解明されていない。本研究で候補受容体として選抜されたFcRY受容体は卵黄のIgY量を決定する鍵因子であり、この受容体の発現レベルと受容体機能の優劣を選抜指標とすれば、高卵黄IgY形質を持つニワトリ系統を開発することが出来る。このニワトリ系統はIgYの増産を可能とし、さらに、ヒナの免疫能が増強されることから、卵を利用した新しい産業の創出に繋がることが期待できる。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (17 results)

All 2020 2019 2018 2017 2016 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (10 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results) Book (2 results) Remarks (1 results)

  • [Journal Article] Evaluation of a receptor gene responsible for maternal blood IgY transfer into egg yolks using bursectomized IgY-depleted chickens.2020

    • Author(s)
      Murai, A., Hamano, T., Kakiuchi, M., Kobayashi, M. and Horio, F.
    • Journal Title

      Poult. Sci.

      Volume: 99 Issue: 4 Pages: 1914-1920

    • DOI

      10.1016/j.psj.2019.11.045

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Ingestion of paddy rice increases intestinal mucin secretion and goblet cell number and prevents dextran sodium sulfate-induced intestinal barrier defect in chickens.2018

    • Author(s)
      5.Murai, A., Kitahara, K., Terada, H., Ueno, A., Ohmori, Y., Kobayashi, M. and Horio, F.
    • Journal Title

      Poult. Sci.

      Volume: 97 Issue: 10 Pages: 3577-3586

    • DOI

      10.3382/ps/pey202

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] An ELISA for quantifying quail IgY and characterizing maternal IgY transfer to egg yolk in several quail strains2016

    • Author(s)
      Murai Atsushi、Kakiuchi Misako、Hamano Takahito、Kobayashi Misato、Tsudzuki Masaoki、Nakano Mikiharu、Matsuda Yoichi、Horio Fumihiko
    • Journal Title

      Veterinary Immunology and Immunopathology

      Volume: 175 Pages: 16-23

    • DOI

      10.1016/j.vetimm.2016.04.013

    • NAID

      120005868237

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Oral antibiotics enhance antibody responses to keyhole limpet hemocyanin in orally but not muscularly immunized chickens2016

    • Author(s)
      Murai, A., Kitahara, K., Okumura, S., Kobayashi, M. and Horio, F.
    • Journal Title

      Anim. Sci. J.

      Volume: 87 Issue: 2 Pages: 257-265

    • DOI

      10.1111/asj.12424

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 加齢による産卵率低下の原因探索:産卵ピーク期とリタイア期での採卵鶏の脂質代謝特性の比較.2019

    • Author(s)
      渡辺駿斗・森大樹・小林美里・堀尾文彦・村井篤嗣
    • Organizer
      日本家禽学会2019年秋季大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 卵黄抗体の輸送機構の解明:FcRY受容体の発現局在と結合特性2019

    • Author(s)
      村井篤嗣・池田光貴・松波華菜子・佐々木諒・小林美里・堀尾文彦
    • Organizer
      日本畜産学会第126回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 採卵鶏における加齢による産卵率低下の原因追求:肝臓と血中の脂質代謝関連パラメーターの変化2019

    • Author(s)
      渡辺駿斗・森大樹・小林美里・堀尾文彦・村井篤嗣
    • Organizer
      令和元年度東海畜産学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 母ドリ卵黄へのIgY抗体の輸送に受容体は関与するのか―FcRY受容体による制御―2019

    • Author(s)
      辰巳郁也・松波華菜子・小林美里・堀尾文彦・村井篤嗣
    • Organizer
      日本畜産学会第125回大会
    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
  • [Presentation] デキストラン硫酸ナトリウムの頻回経口投与がニワトリヒナの腸管バリア機能に及ぼす影響2019

    • Author(s)
      村井篤嗣・森大樹・渡辺駿斗・新居隆浩・小林美里・堀尾文彦
    • Organizer
      日本家禽学会2019年春季大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ニワトリにおけるFcRY受容体の発現局在とIgY-Fc変異体に対する結合活性2018

    • Author(s)
      村井篤嗣・池田光貴・小林美里・堀尾文彦
    • Organizer
      日本家禽学会2018年秋季大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Maternal antibody transfer into eggs and new-born chicks in chicken and quail2018

    • Author(s)
      Murai, A
    • Organizer
      International Symposium on Animal Production and Conservation for Sustainable Development
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] FcRY受容体のIgY-Fc変異体に対する結合活性と卵黄IgY輸送への関与2017

    • Author(s)
      村井篤嗣・垣内美紗子・小林美里・堀尾文彦
    • Organizer
      日本家禽学会2017年度春季大会
    • Related Report
      2016 Annual Research Report
  • [Presentation] Molecular basis of maternal blood IgY transfer into avian egg yolks2016

    • Author(s)
      Murai, A., Hamano, T., Takimoto, T., Kakiuchi, M., Kobayashi, M. and Horio, F.
    • Organizer
      The 17th Asian-Australasian Association of Animal Production Societies Animal Science Congress
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] IgY欠損鶏を活用した卵黄へのIgY輸送を担う受容体の探索2016

    • Author(s)
      垣内美紗子・濱野貴仁・小林美里・堀尾文彦・村井篤嗣
    • Organizer
      平成28年度東海畜産学会秋季大会
    • Related Report
      2016 Annual Research Report
  • [Book] Proceedings of International Symposium on Animal Production and Conservation for Sustainable Development 20182018

    • Author(s)
      Murai, A.
    • Total Pages
      2
    • Publisher
      The United Gradate School of Agricultural Science, Gifu University). Gifu University
    • ISBN
      9784909365033
    • Related Report
      2017 Annual Research Report
  • [Book] 畜産技術:IgY抗体の卵黄への輸送機構の解明とその知見の応用2017

    • Author(s)
      村井篤嗣
    • Total Pages
      11
    • Publisher
      畜産技術協会
    • Related Report
      2016 Annual Research Report
  • [Remarks] 名古屋大学大学院 生命農学研究科 動物栄養科学研究室ホ-ムページ

    • URL

      https://www.agr.nagoya-u.ac.jp/~anutr/

    • Related Report
      2016 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2021-02-19  

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