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Network of flowering proteins in fruit trees

Research Project

Project/Area Number 16K07598
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Horticultural science
Research InstitutionSaga University

Principal Investigator

Kotoda Nobuhiro  佐賀大学, 農学部, 准教授 (50355426)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywordsカンキツ / FT / 相互作用因子 / 酵母ツーハイブリッド / 花成誘導 / 花成抑制 / AP1 / 花芽形成 / 相互作用 / リンゴ / ウンシュウミカン / TFL1/FT / 花成タンパク質 / 糖シグナル / 転写因子 / タンパク質 / FT
Outline of Final Research Achievements

As it is said that Momo-kuri 3 years and kaki 8 years, it takes about 4 to 10 years of juvenile period to produce flowers after sowing seeds of fruit trees. Therefore, flower bud differentiation is an important event for fruit tree production. We have discovered ten or more proteins that bind to the FT protein, based on the analysis of the flowering promoting gene of apple, FLOWING LOCUS T (FT), and the flowering suppression gene, TFL1. In addition, not only the FT protein but also the APETALA1 (AP1) protein, which is putatively related to floral organ formation, binds to other protein and the result suggested its function. Furthermore, we succeeded in identifying the factor that binds to the citrus FT (CuFT) protein.

Academic Significance and Societal Importance of the Research Achievements

花成誘導遺伝子であるFTとの相互作用因子はシロイヌナズナやトマトなどの草本生植物では2000年代初めに報告があったが、果樹等の木本植物では私たちのグループがリンゴで最初に報告をした。今回は、リンゴと同じく主要な果樹であるカンキツにおいて、機能未知のタンパク質がFTと相互作用(結合)することを確認した。FTの機能を直接解明する方法の他に、FTと相互作用する因子を解析することによって、花成の本質を明らかにする方法がある。果樹類の花成は草本植物のように単純ではない可能性があり、このようアプローチから、果樹類の花成メカニズムを明らかにし、将来的には果樹類の花成制御法開発の基礎となることが期待される。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (6 results)

All 2018 2017 2016 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (4 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results) Remarks (1 results)

  • [Journal Article] Gibberellin 2-Oxidase Genes from Satsuma Mandarin (<i>Citrus unshiu</i> Marc.) Caused Late Flowering and Dwarfism in Transgenic <i>Arabidopsis</i>2017

    • Author(s)
      Nobuhiro Kotoda, Satoshi Matsuo, Ichiro Honda, Kanako Yano, Tokurou Shimizu
    • Journal Title

      The Horticulture Journal

      Volume: 86 Issue: 2 Pages: 183-193

    • DOI

      10.2503/hortj.OKD-016

    • NAID

      130005612788

    • ISSN
      2189-0102, 2189-0110
    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Presentation] Phenotypic analysis of transgenic Arabidopsis with GA oxidase like gene CuGA16 isolated from Satsuma mandarin ‘Aoshima’2018

    • Author(s)
      Hasan, N. and N. Kotoda
    • Organizer
      平成30年度園芸学会秋季大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Molecular basis of flowering2017

    • Author(s)
      N. Kotoda and T. Noda
    • Organizer
      ISHS 13th International Symposium
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 北部九州のカンキツと機能性成分2017

    • Author(s)
      N Kotoda
    • Organizer
      Workshop on the Golden Seed Project of Citrus
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] ウンシュウミカン由来花成抑制遺伝子CuBFT, CuCENおよびCuTFL1の機能解析2016

    • Author(s)
      野田孝幸・納冨麻子・古藤田信博
    • Organizer
      園芸学会
    • Place of Presentation
      名城大学
    • Year and Date
      2016-09-10
    • Related Report
      2016 Research-status Report
  • [Remarks] 佐賀大学農学部応用生物科学科果樹園芸学分野

    • URL

      http://fruit.ag.saga-u.ac.jp/HP_kaju1/index.html

    • Related Report
      2017 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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