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Analysis of the mechanism of HSF1 transcription complex by evolutional approach

Research Project

Project/Area Number 16K08625
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Pathological medical chemistry
Research InstitutionYamaguchi University

Principal Investigator

TAKII Ryosuke  山口大学, 大学院医学系研究科, 助教 (00419558)

Research Collaborator FUJIMOTO Mitsuaki  
NAKAI Akira  
MATSUMOTO Masaki  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords熱ショック応答 / HSF / DNAプルダウン / 進化 / 転写 / DNAプルダウン法 / 遺伝子発現 / プロテオーム解析
Outline of Final Research Achievements

To identify the mechanism of HSF1 transcription, we used evolutionary approach. From this results, we identified that two amino acids of HSF1 are necessary to induce HSP70 during heat shock. These two amino acids are conserved form lizard to mammalian HSF1. Finally we identified Factor A by using DNA pulldown assay. Factor A is able to promote HSP70 induction and related cell division.

Academic Significance and Societal Importance of the Research Achievements

細胞内のタンパク質の正しい構造と量は常に一定に保たれている。それらを担う因子の1つが熱ショック転写因子(HSF)である。HSF1は神経変性疾患やがんの病態との関連が報告されているが、治療薬として副作用なくHSF1の活性化を制御する薬剤は現時点ではない。そこで、HSF1転写複合体の構成因子を明らかにし、その活性化機構を解析することは、最終的には上述の病態の改善へつながる。
これまでもHSF1転写複合体の構成因子を明らかにするために、様々な研究者が挑んでいるが強力な制御因子は見つかっていない。そこで、本研究では新たにDNAプルダウン法を通じて、HSF1転写複合体の構成因子群を明らかにする。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (14 results)

All 2018 2017 2016

All Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Open Access: 3 results,  Acknowledgement Compliant: 1 results) Presentation (8 results) (of which Int'l Joint Research: 1 results) Book (1 results)

  • [Journal Article] PARP1 promotes the human heat shock response by facilitating HSF1 binding to DNA2018

    • Author(s)
      Fujimoto Mitsuaki、Takii Ryosuke、Katiyar Arpit、Srivastava Pratibha、Nakai Akira
    • Journal Title

      Molecular and Cellular Biology

      Volume: 38 Issue: 13

    • DOI

      10.1128/mcb.00051-18

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inhibition of gingipains prevents Porphyromonas gingivalis-induced preterm birth and fetal death in pregnant mice.2018

    • Author(s)
      Takii R, Kadowaki T, Tsukuba T, Yamamoto K.
    • Journal Title

      European Journal of Pharmacology

      Volume: 824 Pages: 48-56

    • DOI

      10.1016/j.ejphar.2018.01.028

    • Related Report
      2018 Annual Research Report 2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] HSF1 and HSF3 cooperatively regulate the heat shock response in lizards2017

    • Author(s)
      Takii Ryosuke、Fujimoto Mitsuaki、Matsuura Yuki、Wu Fangxu、Oshibe Namiko、Takaki Eiichi、Katiyar Arpit、Akashi Hiroshi、Makino Takashi、Kawata Masakado、Nakai Akira
    • Journal Title

      PLOS ONE

      Volume: 12 Issue: 7 Pages: e0180776-e0180776

    • DOI

      10.1371/journal.pone.0180776

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] The HSF1-PARP13-PARP1 complex facilitates DNA repair and promotes mammary tumorigenesis2017

    • Author(s)
      Fujimoto Mitsuaki、Takii Ryosuke、Takaki Eiichi、Katiyar Arpit、Nakato Ryuichiro、Shirahige Katsuhiko、Nakai Akira
    • Journal Title

      Nature Communications

      Volume: 8 Issue: 1 Pages: 1638-1638

    • DOI

      10.1038/s41467-017-01807-7

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Anti-TNF-α agent infliximab and splenectomy are protective against renal ischemia-reperfusion injury.2016

    • Author(s)
      Y. Nagata, M. Fujimoto, K. Nakamura, N. Isoyama, M. Matsumura, K. Fujikawa, K. Uchiyama, E. Takaki, R. Takii, A. Nakai, and H. Matsuyama.
    • Journal Title

      Transplantation

      Volume: 100 Issue: 8 Pages: 1675-1682

    • DOI

      10.1097/tp.0000000000001222

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] 染色体関連因子による熱ショック遺伝子の転写誘導機構の解明2018

    • Author(s)
      瀧井良祐、藤本充章、Arpit Katiyar、Pratibha Srivastava、中井 彰
    • Organizer
      第41回日本分子生物学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] トカゲHSF1とHSF3は熱ショック応答を協調的に制御する2018

    • Author(s)
      瀧井良祐、藤本充章、Arpit Katiyar、Pratibha Srivastava、中井 彰
    • Organizer
      第91回日本生化学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] HSF1転写複合体解析による熱ショック応答機構の解明2017

    • Author(s)
      瀧井良祐、藤本充章、Arpit Katiyar、松本雅記、中井 彰
    • Organizer
      転写研究会 冬の若手ワークショップ
    • Place of Presentation
      九十九里浜、千葉県 (一宮シーサイドオーツカ)
    • Year and Date
      2017-01-31
    • Related Report
      2016 Research-status Report
  • [Presentation] 染色体関連因子による熱ショック応答の制御2017

    • Author(s)
      瀧井良祐、中井 彰
    • Organizer
      生命科学系学会合同年次大会
    • Related Report
      2017 Research-status Report
  • [Presentation] HSF1の活性調節に働くリン酸化部位Ser326は進化的に保存されている2017

    • Author(s)
      瀧井良祐、藤本充章、Arpit Katiyar、Pratibha Srivastava、中井 彰
    • Organizer
      第12回臨床ストレス応答学会
    • Related Report
      2017 Research-status Report
  • [Presentation] HSF1 and HSF3 cooperatively regulate the heat shock response in lizards2017

    • Author(s)
      Ryosuke Takii, Mitsuaki Fujimoto, Arpit Katiyar, Pratibha Srivastava and Akira Nakai
    • Organizer
      8th International Congress on Stress Responses in Biology & Medicine
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] HSF1転写複合体の同定と機能解析2016

    • Author(s)
      瀧井良祐、藤本充章、Arpit Katiyar、松本雅記、中井 彰
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      横浜市、神奈川県 (パシフィコ横浜)
    • Year and Date
      2016-11-30
    • Related Report
      2016 Research-status Report
  • [Presentation] 進化的アプローチによる熱ショック因子HSF1転写複合体の同定2016

    • Author(s)
      瀧井良祐、藤本充章、高木栄一、Arpit Katiyar、松本雅記、中井 彰
    • Organizer
      第89回日本生化学会
    • Place of Presentation
      仙台市、宮城県 (仙台国際センター)
    • Year and Date
      2016-09-25
    • Related Report
      2016 Research-status Report
  • [Book] Heat Shock Factor.2016

    • Author(s)
      Takii R, Fujimoto M
    • Total Pages
      301
    • Publisher
      Springer, Japan
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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