| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
Entamoeba histolytica and non-virulent Entamoeba dispar lectins (Igls) have 2 isoforms, Igl1 and Igl2, and both isoforms showed hemolytic activities. The expression levels of Igl2 in Entamoeba histolytica and Entamoeba dispar are equivalent but those of Igl1 are higher in Entamoeba histolytica than in Entamoeba dispar. Therefore, we attenuated expression of Igl1 in Entamoeba histolytica utilizing a gene-silencing technique and evaluated the effect on hemolytic activity. The gene-silenced strain had less hemolytic activity compared with that of wild type indicating that the expression level of Igl1 affected the hemolytic activity of Entamoeba histolytica.
The hemolytic activity resided in 60 amino acids of Igl1 was confirmed from the hemolytic assay using recombinant fragment proteins of Igl1.
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