Specific traffic system mediated by lipid in Entamoeba histolytica
| Project/Area Number |
16K08768
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Parasitology (including sanitary zoology)
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
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| Research Collaborator |
Nakasone Eiko
Hanadate Yuki
Kawano Tetsuro
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 赤痢アメーバ / メンブラントラフィック / 小胞体 / Rab GTPase / 細胞表面 / 細胞内輸送 / Cdc50 / Entamoeba histolytica / 輸送シグナル / 感染症 |
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| Outline of Final Research Achievements |
In enteric protozoan parasite Entamoeba histolytica, EhRab8A GTPase is localized to the ER and needed for the correct transport of surface molecules, which might be needed for the cellular adhesion. In this work, I identified one of the binding protein of EhRab8A, homologue of Cdc50. In mammal, Cdc50 codes a non-catalytic subunit of lipid flippase P4-ATPase and localized to the plasma membrane. EhCdc50-overexpressing transformant amoeba showed that the accumulation of EhCdc50 to the amoebic ER and showed miltefosine resistance, that is broad anti-parasitic phosphocholine analogue. As a second EhRab8A dependent cargo protein, novel molecule EHI_159620 was identified by the biotinylation of constitutively-active EhRab8A expressing cells. Identification of these novel binding proteins of EhRab8A may reveal diverse traffic system in protozoa.
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| Academic Significance and Societal Importance of the Research Achievements |
一般的な分泌タンパク質は、小胞体でCOPII小胞に包まれ、ゴルジ体を経て細胞表面に輸送される。COPII小胞に包まれるには、Sar1 GTPaseの機能が必要である。本研究では嫌気性寄生原虫の赤痢アメーバでは、RabファミリーのGTPaseが小胞体に局在し、小胞体から細胞表面への輸送を担う経路があることを発見した。この現象は、原虫におけるunconventional secretion の存在を示し、多様な輸送機構の理解に貢献する。
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Report
(4 results)
Research Products
(16 results)
