| Project/Area Number |
16K10971
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Anesthesiology
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| Research Institution | Nara Medical University |
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Principal Investigator |
Egawa Junji 奈良県立医科大学, 医学部, 講師 (00453168)
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| Co-Investigator(Kenkyū-buntansha) |
井上 聡己 奈良県立医科大学, 医学部, 病院教授 (50295789)
川口 昌彦 奈良県立医科大学, 医学部, 教授 (60275328)
瓦口 至孝 奈良県立医科大学, 医学部, 非常勤講師 (90433333)
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| Project Period (FY) |
2016-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 頭部外傷 / ミノサイクリン / 欠損体積 / Inverted grid test |
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| Outline of Final Research Achievements |
We investigated whether the direct injection of minocycline in the brain can improve the functional outcome after traumatic brain injury (TBI). We used the C57BL/6 mice for our experiment. Mice were subjected to TBI using a stereotaxic impactor. Immediately after TBI, minocycline or saline (control) was directory injected into the lesion area. The mice injected minocycline tend to maintain the better motor function compared to the saline injected mice (control group), but it is not statistically significant. There is no significant difference in the lesion volume between minocycline group and control group. We did not find any difference in immunohistochemistry images labeling with GFAP (glial fibrillary acidic protein) and Iba1 (ionized calcium-binding adapter molecule 1) between minocycline group and control group. We also did not find the difference in western blot labeling with GFAP between the groups.
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| Academic Significance and Societal Importance of the Research Achievements |
頭部外傷の治療方法をマウスの頭部外傷モデルを使って検討した。炎症を抑えることを目的に、ミノサイクリンという抗生物質を脳に直接投与した。ミノサイクリンを投与したマウスでは、やや運動機能が保持されている傾向にあったが、統計学的には有意なものではなかった。組織や生化学検査も行ったが、大きな差はなかった。今後さらなる投与方法や薬物の研究が必要である。
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