The role of sphingosine-1-phosphate in interstitial fluid promoting the breast cancer progression
| Project/Area Number |
16K19888
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General surgery
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| Research Institution | Niigata University |
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Principal Investigator |
Tsuchida Junko 新潟大学, 医歯学総合病院, 医員 (90769415)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 脂質メディエーター / スフィンゴシン-1-リン酸 / 乳癌 / 癌間質液 / スフィンゴシン-1リン酸 |
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| Outline of Final Research Achievements |
Sphingosine-1-phosphate (S1P) is a bioactive lipid mediator that regulates processes important for cancer progression. We studied the role of S1P in the tumor microenvironment utilizing the experiments using our improved centrifugation method to collect breast tumor and normal tissue interstitial fluid (IF). Importantly, levels of S1P in IF from mammary tumors were reduced when tumor growth was suppressed by oral administration of FTY720/fingolimod. S1P levels were significantly higher in human breast tumor tissue IF than in the normal breast tissue IF. S1P tumor IF measurement illuminates new aspects of the role of S1P in the tumor microenvironment.
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| Academic Significance and Societal Importance of the Research Achievements |
癌間質液中のS1P濃度を定量する方法がこれまでになく、研究はなされてこなかった。我々は、低速遠心分離による組織間質液抽出法を開発し、質量分析装置を用いて、間質液中のS1P濃度を定量することに成功した。癌間質液中のS1Pが癌の発育進展に関わっていることが示唆される結果が得られた。本研究はS1Pの癌微小環境における役割を検討する上で新たな視点をもたらしたと言える。
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Report
(4 results)
Research Products
(23 results)
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[Journal Article] Interstitial Fluid Sphingosine-1-Phosphate in Murine Mammary Gland and Cancer and Human Breast Tissue and Cancer Determined by Novel Methods.2016
Author(s)
Nagahashi M, Yamada A, Miyazaki H, Allegood JC, Tsuchida J, Aoyagi T, Huang WC, Terracina KP, Adams BJ, Rashid OM, Milstien S, Wakai T, Spiegel S, Takabe K.
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Journal Title
J Mammary Gland Biol Neoplasia
Volume: 21
Issue: 1-2
Pages: 9-17
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Presentation] Levels of sphingolipids in plasma are elevated in breast cancer patients with lymph node metastasis2019
Author(s)
Junko Tsuchida, Masayuki Nagahashi, Kazuki Moro, Mayuko Ikarashi, Yu Koyama, Hiroshi Ichikawa, Yoshifumi Shimada, Jun Sakata, Takashi Kobayashi, Hitoshi Kameyama, Kazuaki Takabe, Toshifumi Wakai
Organizer
The 14th Annual Academic Surgical Congress
Related Report
Int'l Joint Research
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[Presentation] Peri-tumor Sphingosine-1-phosphate Levels Are Affected by the Tumor2017
Author(s)
Tsuchida J, Moro K, Ohtani A, Endo M,Niwano T, Tatsuda K, Toshikawa C, Hasegawa M, Ikarashi M, Nakajima M, Y. Koyama Ｙ, Sakata J, Kobayashi T, Kameyama H, Takabe K, Wakai T, Nagahashi M
Organizer
The 12th Annual Academic Surgical Congress
Place of Presentation
Las Vegas, USA
Related Report
Int'l Joint Research
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[Presentation] Obesity-induced Sphingosine-1-phosphate in Breast Cancer Interstitial Fluid Promotes Angiogenesis2017
Author(s)
Tsuchida J, Nakajima M, Moro K, Ohtani A, Endo M, Niwano T, Tatsuda K, Toshikawa C, Hasegawa M, Ikarashi M, Koyama Y, Sakata J, Kobayashi T, Kameyama H, Wakai T, Takabe K, Nagahashi M
Organizer
The 12th Annual Academic Surgical Congress
Place of Presentation
Las Vegas, USA
Related Report
Int'l Joint Research
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