Molecular basis study for revealing the pathogenesis of NAFLD after pancreatic resection focusing on extracellular matrix

• Project/Area Number: 16K19917
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Digestive surgery
• Research Institution: Mie University
• Principal Investigator: Kato Hiroyuki 三重大学, 医学部附属病院, 助教 (50737004)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 膵頭十二指腸切除 / 細胞外マトリックス / NAFLD / マウス / ・ラット / 脂肪肝 / 膵外分泌機能

Outline of Final Research Achievements

We performed the study to prove the etiology of development of NASH after a pancreaticoduodenectomy (PD) using mice or rat PD models. Even in mice and rat PD model, significant liver injury and fat deposition to the liver parenchyma and sinusoidal injury as evidenced by PECAM-1 depression were seen at the 24 hours after PD. Nevertheless, we observed the phenomenon that mice and rat markedly lost their appetite, resulting in death. However, In rat PD model, the level of TNF-a mRNA, neutrophil infiltration and monocytes infiltration in liver are significantly potentiated, with compared to the SHAM model.In conclusion, MMP-9 upregulation might be associated with the development of NAFLD after PD.

Research Products

• [Journal Article] Different roles of pancreatic duct dilatation and remnant pancreatic volume for the development of pancreatic endocrine and exocrine dysfunction. (2017)
  • Author(s): Iizawa Y, Kato H, Kishiwada M, Hayasaki A, Tanemura A, Murata Y, Azumi Y, Kuriyama N, Mizuno S, Usui M, Sakurai H, Isaji S.
  • Journal Title: pancreatology Volume: 17 Issue: 5 Pages: 814-821
  • DOI: 10.1016/j.pan.2017.06.008
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Book] 膵頭十二指腸切除（PD）後の脂肪肝 (2018)
  • Author(s): 坂口充弘、飯澤祐介、藤井武宏、加藤宏之、伊佐地秀司
  • Publisher: 医学図書出版