• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

New unknown phenomena of anaerobic wastewater treatment : understanding and control of anaerobic bulking by multiple species of microorganisms

Research Project

Project/Area Number 17H03333
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Civil and environmental engineering
Research InstitutionToyohashi University of Technology

Principal Investigator

Yamada Takeshi  豊橋技術科学大学, 工学(系)研究科(研究院), 講師 (90533422)

Co-Investigator(Kenkyū-buntansha) 山口 剛士  松江工業高等専門学校, 環境・建設工学科, 准教授 (30759832)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2019: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2018: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2017: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
KeywordsEGSB / 嫌気性バルキング / アナエロリネア綱細菌 / メタノサエタ属アーキア / ドラフトゲノム / EGSBリアクター / 糸状性微生物 / 空間分布 / ゲノム / クロロフレキシ門細菌 / スピアマン順位相関係数 / 微生物蛍光検出法 / 嫌気性廃水処理 / バクテロイデス / クロロフレキシ / HCR-FISH / 環境技術
Outline of Final Research Achievements

A sudden outflow of granule sludge (anaerobic bulking) has been reported with the spread of expanded granular sludge bed reactors and the expansion of applied wastewater species. In order to overcome this problem, it is essential to elucidate the causative bacteria involved in this problem and establish control technology based on that information. In this study, we estimated the substrate utilization of causative bacteria for anaerobic bulking, and succeeded in the isolation of the causative bacteria. We also succeeded in constructing the draft genome required for estimating the bulking mechanism.

Academic Significance and Societal Importance of the Research Achievements

本研究では、Expanded granular sludge bed (EGSB) リアクターの問題となっていた嫌気性バルキング現象に焦点を当て、その制御方法を確立するため、バルキングメカニズムの解明に関する研究を試みた。嫌気性バルキングに関与する糸状性細菌は分離・培養が困難な難培養微生物であったが、本研究を通して分離とそのドラフトゲノムの解読に成功した。これらの成果は、EGSBリアクターの安定的な運転を実現する上で必要となる制御技術の確立に先鞭を付ける成果であり、その学術的・社会的意義は大きいと考えている。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (30 results)

All 2019 2018 2017

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 4 results) Presentation (17 results) (of which Int'l Joint Research: 2 results) Book (8 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Draft Genome Sequence of Thermodesulfovibrio sp. Strain Kuro-1, a Thermophilic, Lactate-Degrading Anaerobe Isolated from a Thermophilic Anaerobic Digestion Reactor2019

    • Author(s)
      Takeshi Yamada, Jun Harada, Misaki Kurobe, Surya Giri, Masaru Konishi Nobu, Takashi Narihiro, Hideto Tsuji, Hiroyuki Daimon
    • Journal Title

      Microbiology Resource Announcements

      Volume: 8 Issue: 29

    • DOI

      10.1128/mra.00709-19

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 16S rRNA Gene Amplicon Profiling of Anaerobic Bulking- Associated Prokaryotic Microbiota in a Mesophilic Expanded Granular Sludge Bed Reactor for Beverage Wastewater Treatment2019

    • Author(s)
      Takeshi Yamada, Jun Harada, Yuki Okazaki, Tsuyoshi Yamaguchi, Atsushi Nakano
    • Journal Title

      Microbiology Resource Announcements

      Volume: 8 Issue: 30

    • DOI

      10.1128/mra.00678-19

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 16S rRNA Gene Amplicon Sequencing of Microbial Communities Involved in Anaerobic Bulking in a Mesophilic Expanded Granular Sludge Bed Reactor Treating Wastewater Discharged from a Japanese-Style Thickened Worcestershire Sauce-Producing Factory2019

    • Author(s)
      Takeshi Yamada, Jun Harada, Yuki Okazaki, Tsuyoshi Yamaguchi, Atsushi Nakano
    • Journal Title

      Microbiology Resource Announcements

      Volume: 8 Issue: 36

    • DOI

      10.1128/mra.00801-19

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Draft Genome Sequence of Moorella sp. Strain Hama-1, a Novel Acetogenic Bacterium Isolated from a Thermophilic Digestion Reactor2018

    • Author(s)
      Harada Jun、Yamada Takeshi、Giri Surya、Hamada Masako、Nobu Masaru K.、Narihiro Takashi、Tsuji Hideto、Daimon Hiroyuki
    • Journal Title

      Genome Announcements

      Volume: 6 Issue: 24

    • DOI

      10.1128/genomea.00517-18

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Morphology and Distribution of Causative Microorganisms involved in Anaerobic Bulking in the EGSB Reactor2019

    • Author(s)
      Jun Harada, Tsuyoshi Yamaguchi, Takashi Narihiro, Atsushi Nakano, Takeshi Yamada
    • Organizer
      Water and Environment Technology Conference 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 食品系有機性廃水を処理するEGSB リアクターの新たな嫌気性バルキングの原因解明2019

    • Author(s)
      原田淳, 岡﨑祐輝, 山口剛士, 成廣隆, 中野淳, 山田剛史
    • Organizer
      令和元年度土木学会全国大会 第74回年次学術講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] EGSB リアクターの嫌気性バルキングに関与する未培養糸状性微生物の分離と培養2019

    • Author(s)
      原田淳, 山口剛士, 成廣隆, 中野淳, 山田剛史
    • Organizer
      日本微生物生態学会第33回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] EGSBリアクターで発生した嫌気性バルキングに関与する糸状性細菌のグラニュール汚泥内空間分布の評価2019

    • Author(s)
      原田淳, 山口剛士, 成廣隆, 中野淳, 山田剛史
    • Organizer
      第56回環境工学研究フォーラム
    • Related Report
      2019 Annual Research Report
  • [Presentation] 高感度FISH法を用いた微生物回収の試み2019

    • Author(s)
      岡﨑祐輝, 山口剛士, 原田淳, 山田剛史, 中野淳
    • Organizer
      第53回日本水環境学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] EGSBリアクターで発生した嫌気性バルキングのバルキング原因菌の同定と原因解明2019

    • Author(s)
      原田淳, 岡﨑祐輝, 山口剛士, 中野淳, 山田剛史
    • Organizer
      第53回日本水環境学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] EGSBリアクターにおける数種の未培養糸状性細菌群が関与する嫌気性バルキング2018

    • Author(s)
      原田淳, 岡﨑祐輝, 浜田雅子, 山口剛士, 中野淳, 成廣隆, 山田剛史
    • Organizer
      日本微生物生態学会第32回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] EGSBリアクター内の汚泥に対するHCR-FISH法を用いた未培養微生物の視覚的検出2018

    • Author(s)
      岡﨑祐輝, 山口剛士, 原田淳, 山田剛史, 中野淳
    • Organizer
      日本微生物生態学会第32回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] EGSBリアクターの新たな嫌気性バルキングに関与する複数種の嫌気性バルキング原因菌の解明2018

    • Author(s)
      原田淳, 岡﨑祐輝, 浜田雅子, 山口剛士, 中野淳, 成廣隆, 山田剛史
    • Organizer
      土木学会全国大会 第73回年次学術講演会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 分子生物学的技法と統計解析を用いたEGSBリアクターにおける新たな嫌気性バルキングの原因解明2018

    • Author(s)
      原田淳, 岡﨑祐輝, 山口剛士, 中野淳, 山田剛史
    • Organizer
      平成30年度日本水環境学会中部支部研究発表会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Identification of causative filamentous bacteria for anaerobic bulking occurred in an EGSB reactor2018

    • Author(s)
      Harada, J., Y. Okazaki, T. Yamaguchi, A. Nakano, T. Yamada
    • Organizer
      Irago conference 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] EGSBリアクターの新たな嫌気性バルキング:複数種の原因微生物の関わりを視覚的に捉える2018

    • Author(s)
      原田淳, 浜田雅子, 岡崎祐輝, 山口剛士, 中野淳, 山田剛史
    • Organizer
      第52回日本水環境学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] EGSBリアクター内に生息する未培養微生物の視覚的検出2018

    • Author(s)
      岡﨑祐輝, 原田淳, 山田剛史, 中野淳, 山口剛士
    • Organizer
      第52回日本水環境学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 嫌気性廃水処理汚泥のバルキングに関与する未培養微生物群の解析2017

    • Author(s)
      山田剛史, 原田淳, 岡﨑祐輝, 浜田雅子, 山口剛士, 中野淳
    • Organizer
      環境微生物系学会合同大会2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] 高感度FISH法を用いた水処理装置内の未培養微生物の視覚的検出2017

    • Author(s)
      岡﨑祐輝, 山口剛士, 中野淳, 山田剛史
    • Organizer
      土木学会全国大会 第72回年次学術講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 嫌気性排水処理を行うEGSBリアクターでの嫌気性バルキングに関する微生物の検出2017

    • Author(s)
      原田淳, 山田剛史
    • Organizer
      東三河生態系ネットワークフォーラム2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] 食品系有機性廃水を処理するEGSBリアクターで発生した嫌気性バルキングに関する微生物評価2017

    • Author(s)
      山田剛史, 原田淳, 岡崎祐輝, 浜田雅子, 山口剛士, 中野淳
    • Organizer
      第54回環境工学研究フォーラム
    • Related Report
      2017 Annual Research Report
  • [Book] "Anaerolinea", in Bergey’s Manual of Systematics of Archaea and Bacteria (BMSAB), Whitman, W.B. (Eds.)2018

    • Author(s)
      Yamada, T. and Y. Sekiguchi
    • Total Pages
      5
    • Publisher
      John Wiley
    • Related Report
      2018 Annual Research Report
  • [Book] "Anaerolineaceae", in Bergey’s Manual of Systematics of Archaea and Bacteria (BMSAB), Whitman, W.B. (Eds.)2018

    • Author(s)
      Yamada, T and Y. Sekiguchi
    • Total Pages
      4
    • Publisher
      John Wiley
    • Related Report
      2018 Annual Research Report
  • [Book] "Anaerolineae", in Bergey’s Manual of Systematics of Archaea and Bacteria (BMSAB), Whitman, W.B. (Eds.)2018

    • Author(s)
      Yamada, T and Y. Sekiguchi
    • Total Pages
      2
    • Publisher
      John Wiley
    • Related Report
      2018 Annual Research Report
  • [Book] "Bellilinea", in Bergey’s Manual of Systematics of Archaea and Bacteria (BMSAB), Whitman, W.B. (Eds.)2018

    • Author(s)
      Yamada, T. and Y. Sekiguchi
    • Total Pages
      3
    • Publisher
      John Wiley
    • Related Report
      2017 Annual Research Report
  • [Book] "Leptolinea", in Bergey’s Manual of Systematics of Archaea and Bacteria (BMSAB), Whitman, W.B. (Eds.)2018

    • Author(s)
      Yamada, T. and Y. Sekiguchi
    • Total Pages
      4
    • Publisher
      John Wiley
    • Related Report
      2017 Annual Research Report
  • [Book] "Levilinea", in Bergey’s Manual of Systematics of Archaea and Bacteria (BMSAB), Whitman, W.B. (Eds.)2018

    • Author(s)
      Yamada, T. and Y. Sekiguchi
    • Total Pages
      4
    • Publisher
      John Wiley
    • Related Report
      2017 Annual Research Report
  • [Book] "Longilnea", in Bergey’s Manual of Systematics of Archaea and Bacteria (BMSAB), Whitman, W.B. (Eds.)2018

    • Author(s)
      Yamada, T. and Y. Sekiguchi
    • Total Pages
      3
    • Publisher
      John Wiley
    • Related Report
      2017 Annual Research Report
  • [Book] "Anaerolineales", in Bergey’s Manual of Systematics of Archaea and Bacteria (BMSAB), Whitman, W.B. (Eds.)2018

    • Author(s)
      Yamada, T. and Y. Sekiguchi
    • Total Pages
      1
    • Publisher
      John Wiley
    • Related Report
      2017 Annual Research Report
  • [Patent(Industrial Property Rights)] アンモニア酸化細菌に結合する核酸分子、その核酸分子を用いるアンモニア酸化細菌の検出方法及び検出キット2018

    • Inventor(s)
      山田剛史、萩原達也
    • Industrial Property Rights Holder
      山田剛史、萩原達也
    • Industrial Property Rights Type
      特許
    • Filing Date
      2018
    • Related Report
      2017 Annual Research Report

URL: 

Published: 2017-04-28   Modified: 2021-02-19  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi