Survey and development of rapid test for acaricide resistance in ticks in Kenya and Uganda
| Project/Area Number |
17H04641
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 海外学術 |
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| Research Field |
Veterinary medical science
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
白藤 梨可 帯広畜産大学, 原虫病研究センター, 助教 (00549909)
中尾 亮 北海道大学, 獣医学研究院, 准教授 (50633955)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥15,080,000 (Direct Cost: ¥11,600,000、Indirect Cost: ¥3,480,000)
Fiscal Year 2019: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
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| Keywords | R. decoloratus / 殺ダニ剤抵抗性 / ケニア / ウガンダ / amitraz / octopamine receptor / SNP / LAMP-RFLP / マダニ / アフリカ / cell-based assay / ガーナ / Acetylcholinesterase / 電位依存性ナトリウムチャネル / アドレナリン作動性様オクトパミン受容体 |
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| Outline of Final Research Achievements |
In the promotion of livestock economy in the African region, the blood-feeding of Rhipicephalus decoloratus and the spread of tick-borne infection are serious obstacles. This study aims to develop a rapid technique for diagnosing acaricide-resistance in tick, which will solve the problem of conventional diagnostic bioassay protocols. In particular, this study was performed focusing on the mutation of the octopamine receptor gene encoding the target molecule of amitraz, a formamidine-based acaricide. As a result of SNP analysis, so far, we found at least 3 SNPs with amino acid substitutions, and succeeded in finding one SNP mutation conferring higher production of cAMP than that of wild type in cAMP analysis.
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| Academic Significance and Societal Importance of the Research Achievements |
殺ダニ剤抵抗性を有するRhipicephalus decoloratusは、アフリカ地域優占種ということもあり、遺伝子情報が不足している。殺ダニ剤抵抗性の診断は従来の虫体を使用した暴露試験で行われているが、これに代替しうる遺伝子診断手法が希求されている。本研究成果は、殺ダニ剤アミトラズの抵抗性形質に関与する遺伝子のSNP検出法の開発に寄与するものであり、学術的にも社会的にもインパクトの大きい成果といえる。
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Report
(4 results)
Research Products
(20 results)
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[Journal Article] NovelCharacteristics of Mitochondrial Electron Transport Chain from Eimeria tenella2018
Author(s)
Matsubayashi M, Inaoka DK, Komatsuya K, Hatta T, Kawahara F, Sakamoto K,Hikosaka K, Yamagishi J, Sasai K, Shiba T, Harada S, Tsuji N, Kita K.
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Journal Title
Genes (Basel)
Volume: 10
Issue: 1
Pages: 1-1
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Trematode infections in farm animals and their vector snails in Saint Martin’s Island, the southeastern offshore area of Bangladesh in the Bay of Bengal2018
Author(s)
Yasin MG, Alim MA, Anisuzzaman, Ahasan SA, Munsi MN, Chowdhury EH, Hatta T,Tsuji N, Mondal MMH.
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Journal Title
Journal of Veterinary Medical Science
Volume: 80
Issue: 4
Pages: 684-688
DOI
NAID
ISSN
0916-7250, 1347-7439
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Positive phototropism is accelerated in Biomphalaria glabrata snails by infection with Schistosoma mansoni.2018
Author(s)
H Maeda, T Hatta, D Tsubokawa, F Mikami, T Nishimaki, T Nakamura, Anisuzzaman, M Matsubayashi, M Ogawa, CP da Costa, N Tsuji
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Journal Title
Parasitology International
Volume: 67
Issue: 5
Pages: 609-611
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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