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Prevalence, antimicrobial susceptibility and characterization of typical and atypical EPEC in Thailand and Kenya

Research Project

Project/Area Number 17H04651
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section海外学術
Research Field Bacteriology (including mycology)
Research InstitutionOsaka Prefecture University

Principal Investigator

Yamasaki Shinji  大阪府立大学, 生命環境科学研究科, 教授 (70221653)

Co-Investigator(Kenkyū-buntansha) 日根野谷 淳  大阪府立大学, 生命環境科学研究科, 准教授 (20548490)
飯島 義雄  神戸市環境保健研究所, その他部局等, 所長 (60144739)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥15,600,000 (Direct Cost: ¥12,000,000、Indirect Cost: ¥3,600,000)
Fiscal Year 2019: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2018: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2017: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Keywordseae遺伝子 / 下痢原性大腸菌 / aEPEC / tEPEC / E. albertii / 感染症 / 細菌 / 獣医学
Outline of Final Research Achievements

food samples, in particular, meats in both Thailand and Kenya. However, bundle forming pilus (bfp) gene was not detected in the same sample and even in eae gene-positive Escherichia coli isolated. These data indicate that atypical enteropathogenic E. coli (only eae gene-positive) is prevalent but not typical enteropathogenic E. coli (both eae and bfp gene-positive) in Thailand and Kenya. In addition, Escherichia albertii specific gene such as Eacdt genes were also detected in meat samples as well as stool specimens of diarrheal patients in Thailand. Furthermore, E. albertii was isolated from not only meat samples but also diarrheal patient. The isolates do not contain stx2 genes but produce biological active cytolethal distending toxin. Two E. albertii isolates were multi-drug resistant. Taken together, eae gene-positive bacteria such as aEPEC and E. albertii might be important emerging zoonotic pathogens.

Academic Significance and Societal Importance of the Research Achievements

eae、bfp及びEAF遺伝子が陽性のEPECが、特に開発途上国では下痢症の原因であると考えられてきた。今回、タイ、ケニアにおいてeae遺伝子のみ陽性のaEPECが主流であることが明らかとなった。aEPECはstx2ファージが感染することでEHEC になることからtEPECよりより病原性が強いと言われている。一方、今回初めてE. albertiiをタイの食品のみならず下痢症患者便から検出した。今後、aEPECとtEPECを鑑別できる検査法やE. albertiiの検査法を普及することで、これらの細菌の疫学や下痢症起因菌としての位置づけをより明確にできることが期待できる。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (13 results)

All 2020 2019 2018 2017 Other

All Int'l Joint Research (7 results) Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results) Presentation (1 results)

  • [Int'l Joint Research] Thammasat University(タイ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Technical University of Mombasa(ケニア)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Thammasat University(タイ)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] technical University of Mombasa(ケニア)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Kuwait University(クウェート)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Mombasa University of Technology(ケニア)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Thammassat University(タイ)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Development of XRM-MacConkey agar selective medium for the isolation of Escherichia albertii2020

    • Author(s)
      Hinenoya Atsushi、Nagano Keigo、Okuno Kentaro、Nagita Akira、Hatanaka Noritoshi、Awasthi Sharda Prasad、Yamasaki Shinji
    • Journal Title

      Diagnostic Microbiology and Infectious Disease

      Volume: 97 Issue: 1 Pages: 115006-115006

    • DOI

      10.1016/j.diagmicrobio.2020.115006

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phenotypic and molecular characterization of Escherichia albertii: Further surrogates to avoid potential laboratory misidentification.2019

    • Author(s)
      Hinenoya#, H. Ichimura#, S.P. Awasthi, N. Yasuda, J. Yatsuyanagi and S. Yamasaki
    • Journal Title

      Int. J. Med. Microbiol.

      Volume: 309 Issue: 2 Pages: 108-115

    • DOI

      10.1016/j.ijmm.2018.12.003

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of a specific cytolethal distending toxin (cdt) gene (Eacdt)-based PCR for the detection and identification of Escherichia albertii.2019

    • Author(s)
      A. Hinenoya, H. Ichimura, N. Yasuda, S. Harada, K. Yamada, M. Suzuki, Y. Iijima, N. Hatanaka, S.P. Awasthi, and S. Yamasaki*
    • Journal Title

      Diagn. Microbiol. Infect. Dis.

      Volume: 95 Issue: 2 Pages: 119-124

    • DOI

      10.1016/j.diagmicrobio.2019.04.018

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of a multiplex PCR for the simultaneous detection of eae, stx and cdt genes in genus Escherichia and detection of a novel cdtB gene in Providencia rustigianii2018

    • Author(s)
      J. Hassan, S.P. Awasthi, N. Hatanaka, K. Okuno, P.H. Hoang, A. Nagita, A. Hinenoya and S. Yamasaki
    • Journal Title

      Pathog. Dis.

      Volume: 76 Issue: 9 Pages: 1-7

    • DOI

      10.1093/femspd/ftz002

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Association of cytolethal distending toxin-II gene-positive Escherichia coli with Escherichia albertii , an emerging enteropathogen2017

    • Author(s)
      Hinenoya A, Yasuda N, Mukaizawa N, Sheikh S, Niwa Y, Awasthi SP, Asakura M, Tsukamoto T, Nagita A, Albert MJ, Yamasaki S
    • Journal Title

      Int J Med Microbiol

      Volume: 307 Issue: 8 Pages: 564-571

    • DOI

      10.1016/j.ijmm.2017.08.008

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] タイ王国の市販食品におけるeae遺伝子陽性細菌の汚染状況2019

    • Author(s)
      瀧本誠太、Srinuan Somroop、畑中律敏、Sharda Prasad Awasth、日根野谷 淳、山崎伸二
    • Organizer
      第72回日本細菌学会関西支部総会
    • Related Report
      2019 Annual Research Report

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Published: 2017-04-28   Modified: 2025-01-30  

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