Elucidation and application of the biosynthetic pathway of plasmalogens in gut microbes
| Project/Area Number |
17H05025
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied microbiology
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| Research Institution | Kyoto University |
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Principal Investigator |
Ando Akinori 京都大学, 農学研究科, 助教 (10537765)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥25,740,000 (Direct Cost: ¥19,800,000、Indirect Cost: ¥5,940,000)
Fiscal Year 2019: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2018: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
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| Keywords | 腸内細菌 / plasmalogens / プラズマローゲン / リン脂質 / 脂質 / 微生物 / バイオテクノロジー / 応用微生物 |
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| Outline of Final Research Achievements |
In this study, we conducted to elucidate the plasmalogen biosynthetic mechanism in gut microbes. We revealed that most of plasmalogens were ethanolamine-type plasmalogens (PlsEtn), the top 10 molecular species accounted for about 90% of the total PlsEtn, and no PlsEtn with a polyunsaturated fatty acid bound at the sn-2 position, like human type plasmaloges.
No Δ1'-desaturase homolog gene was found in these strains which accumulated some plasmalogens. The addition of dioleoylphosphatidylethanolamine to the medium did not change the plasmalogen composition. In addition, low correlation between the distribution of molecular species of PE and PlsEtn in these bacteria. These results strongly suggested that the plasmalogen biosynthetic pathway in gut microbes is different from that in humans.
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| Academic Significance and Societal Importance of the Research Achievements |
プラズマローゲンは、生体内では希少なリン脂質ではなくむしろありふれた脂質であるにもかかわらず、いまだ具体的な生理的機能は明らかにされていない。本研究では、生体への供給源となりうる腸内細菌群のプラズマローゲンに着目し、その分布、形態、組成を精査し、さらに生合成経路の解明に取り組んだ。研究成果として得られる機能性微生物・脂質は食品素材・医薬品としての機能が期待されるものであり、それら業界を刺激するものと考えられる。
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Report
(4 results)
Research Products
(40 results)
