| Project/Area Number |
17H06718
|
|---|
| Research Category |
Grant-in-Aid for Research Activity Start-up
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Obstetrics and gynecology
|
|---|
| Research Institution | University of Fukui |
|---|
Principal Investigator |
Miyazaki Yumiko 福井大学, 学術研究院医学系部門(附属病院部), 医員 (10808710)
|
|---|
| Research Collaborator |
Orisaka Makoto
Mizutani Tetsuya
Yazawa Takashi
|
|---|
| Project Period (FY) |
2017-08-25 – 2019-03-31
|
| Project Status |
Completed (Fiscal Year 2018)
|
| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
|---|
| Keywords | 間葉系幹細胞 / 顆粒膜細胞 / 羊膜 / Steroidogenic factor 1 / Steroidogenic Factor 1 / SF-1 / 再生医学 |
|---|
| Outline of Final Research Achievements |
The mechanism by which differentiation occurs from mesoderm to ovary, particularly granulosa cells, is still unclear. We aimed to establish a protocol to induce differentiation of pregranulosa cells efficiently using human amniotic membrane-derived mesenchymal stem cells (hAMMSC). Amniotic membrane was collected from the placenta of pregnant woman who gave consent. Cell sorting was performed using a surface marker of mesenchymal stem cells to separate the human amniotic membrane-derived mesenchymal stem cells. It was confirmed that this hAMMSC has pluripotency to differentiate into osteoblasts and adipocytes. Adenovirus-mediated Steroidogenic factor 1(SF-1) gene transfer allowed hAMMSC to differentiate into cells expressing multiple steroid hormone-related enzyme genes.
|
| Academic Significance and Societal Importance of the Research Achievements |
中胚葉から副腎や精巣が分化するメカニズムはある程度解明されているが、卵巣とくに顆粒膜細胞の分化発生メカニズムは未だ明らかでない。今回我々は、ヒト羊膜由来の間葉系幹細胞（hAMMSC）にSF-1遺伝子を導入することで、ステロイド関連酵素を発現する細胞へと分化させた。さらなる条件の検討により、hAMMSCを前顆粒膜細胞へと誘導することを目指している。ヒト人工多能性幹細胞（iPS細胞）から始原生殖細胞様細胞を誘導し、マウス胎仔卵巣組織と共培養することで、ヒト卵原細胞を分化誘導することが可能となっている。本研究による顆粒膜細胞の作製は、iPS細胞を用いたヒト卵子再生の一助となる可能性がある。
|
