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Metagenomics on Oral Bacteria and Fecal Bacteria using a Next Generation Sequencing System

Research Project

Project/Area Number 17H07200
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Social dentistry
Research InstitutionTsurumi University

Principal Investigator

Otsuka Ryoko  鶴見大学, 歯学部, 学部助手 (00803701)

Project Period (FY) 2017-08-25 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords口腔細菌叢 / 腸内細菌叢 / 炎症性腸疾患
Outline of Final Research Achievements

Oral and fecal microbiota from volunteers having inflammatory bowel disease (IBD) were investigated using an advanced next generation sequencing technology and compared with those of healthy volunteers. Oral and fecal samples of ten healthy volunteers and 12 IBD volunteers were sequenced after extracting whole DNA using a 16S rRNA sequencing on the Ion Torrent Personal Genome Machine (I-PGM) System.
Streptococcus salivarius and Streptococcus oralis were detected in both oral and fecal samples collected from most volunteers in either group. Streptococcus mutans, Streptococcus gordonii and Actinomyces spp. were present in both samples of few volunteers. Granulicatella adiacens and Streptococcus salivarius were detected in both oral and fecal samples collected from three IBD volunteers. All volunteers from both groups had Streptococcus sinensis in their oral cavity. With few exceptions most volunteers had Streptococcus sinensis in their fecal samples.

Academic Significance and Societal Importance of the Research Achievements

近年、IBDの患者数は急増しているにも関わらず、根本原因がわからないため、効果的な予防方法や根治療法は確立していない。本研究によりIBD患者の口腔および腸内細菌叢から細菌学的な知見が得られたことにより、予防方法や根治療法の発見の一助となることが期待される。

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • Research Products

    (13 results)

All 2019 2018 2017

All Journal Article (2 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (10 results) (of which Int'l Joint Research: 2 results) Book (1 results)

  • [Journal Article] Precede-Proceed model based questionnaire and saliva tests for oral health checkup in adult2019

    • Author(s)
      Yoshiaki Nomura, Tomoaki Matsuyama, Kakuhiro Fukai, Ayako Okada, Mitsuo Ida, Noriaki Yamauchi, Hiroyuki Hanamura, Yoshihide Yabuki, Kazuhiko Watanabe, Motoko Sugawara, Yasuhiko Imanishi, Norihiro Koizumi, Yoshinori Murano, Atsushi Nishiyama, Yoshiteru Fukukawa, Ryoko Otsuka, Nobuhiro Hanada.
    • Journal Title

      Journal of Oral Science

      Volume: in press

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Confocal Laser Microscopic Images of In Vitro Cariogenic Biofilm and Resulted Demineralized Dentin2018

    • Author(s)
      Khairul Matin, Thwe Zin Ei, Ryoko Otsuka, Ayako Okada, Kenichi Nebuka, Nobuhiro Hanada, Junji Tagami
    • Journal Title

      Journal of Oral Health & Dentistry

      Volume: 1 Pages: 39-41

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] がん治療周術期における口腔内環境の変化―1症例報告 第2報2018

    • Author(s)
      大塚良子, 今井奨, 岡田彩子, 有吉芽生, 菊地朋宏, 宮之原真由, 曽我部薫, 村田貴俊, マティン カイルール, 野村義明, 花田信弘, 桃井保子, 熊谷崇
    • Organizer
      第67回日本口腔衛生学会・総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 血管内皮機能に対する歯周組織健康状態改善の有効性評価:ランダム化並行群間比較試験(第一報)2018

    • Author(s)
      有吉芽生, 村田貴俊, 岡田彩子, 大塚良子, 山下万美子, 鈴木恵, 青柳ひとみ, 植松裕美, 今村安芸子, 佐藤勉, カイルール マティン, 花田信弘
    • Organizer
      第67回日本口腔衛生学会・総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 喫煙者の口腔ケアと歯周組織の健康状態との関連性について2018

    • Author(s)
      岡田彩子, 村田貴俊, 有吉芽生, 大塚良子, 山下万美子, 鈴木恵, 青柳ひとみ, 植松裕美, 今村安芸子, 佐藤勉, カイルール マティン, 花田信弘
    • Organizer
      第67回日本口腔衛生学会・総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 唾液を用いた歯周病のスクリーニング検査による地域健康診断システム構築~平成29年度までの実施結果報告~2018

    • Author(s)
      上谷公之, 大祢貴俊, 矢吹義秀, 福澤洋一, 古藤真実, 吉野浩和, 三穂乙暁, 西辻直之, 谷村秀樹, 長井博昭, 小石健, 大塚良子, 岡田彩子, 岡本公彰, 野中衣理加, 村田貴俊, 野村義明, 花田信弘
    • Organizer
      第67回日本口腔衛生学会・総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 隣接面のプラークコントロールに関する研究 第10報:ゴム製歯間ブラシによる人工歯隣接面のプラーク除去率2018

    • Author(s)
      引地清水, 大塚良子, 岡田彩子, 桃井保子
    • Organizer
      日本歯科保存学会2018年度春期学術大会 第148回
    • Related Report
      2018 Annual Research Report
  • [Presentation] Metagenomics of Oral and Intestinal Bacteria using a Next Generation Sequencing System; Preliminary Report2018

    • Author(s)
      Ryoko Otsuka, Khairul Matin, Ayako Okada, Meu Ariyoshi, Kosuke Tanimoto, Junji Tagami, Nobuhiro Hanada
    • Organizer
      The 66th Annual Meeting of Japanese Association for Dental Research
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The Random Allocation Process in a Randomized Clinical Trial;Effectiveness of Periodontal Health Improvement on Vascular Endothelial Function2018

    • Author(s)
      Ayako Okada, Takatoshi Murata, Meu Ariyoshi, Ryoko Otsuka, Mamiko Yamashita, Miki Kosaka, Masayuki Suzuki, Ken Tateno, Rumi Wakiyama, Megumi Suzuki, Hitomi Aoyagi, Hiromi Uematsu, Akiko Imamura, Tsutomu Sato, Hiroshi Kawahara, Khairul Matin, Nobuhiro Hanada
    • Organizer
      The 66th Annual Meeting of Japanese Association for Dental Research
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 血管内皮機能に対する歯周組織健康状態改善の有効性評価:ランダム化並行群間比較試験(ベースライン分析)2018

    • Author(s)
      村田貴俊,岡田彩子,有吉芽生,大塚良子,山下万美子,鈴木 恵,青柳ひとみ,植松裕美,今村安芸子,佐藤 勉,マティン カイル-ル,花田信弘.
    • Organizer
      第19回日本口腔機能水学会・学術大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] がん治療周術期における口腔内環境の変化1症例報告2017

    • Author(s)
      大塚良子,今井 奬,阿部 繭,岡田彩子,有吉芽生,菊地朋宏,村田貴俊,マティン カイルール,野村義明,花田信弘,桃井保子,熊谷 崇.
    • Organizer
      第66回日本口腔衛生学会・総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Liquid Carrier Type 3DSトレーを用いた殺菌洗口液の細菌学的・臨床的効果2017

    • Author(s)
      岡田彩子,有吉芽生,曽我部薫,大塚良子,宮之原真由,村田貴俊,マティン カイルール,花田信弘.
    • Organizer
      第66回日本口腔衛生学会・総会
    • Related Report
      2017 Annual Research Report
  • [Book] 患者さんへの“ベストアンサー”シリーズ11 “老化の予防”歯科Q&A 口の健康が生活習慣病を防ぎます2018

    • Author(s)
      武内博朗,野村義明,花田信弘,今井 奨,大塚良子,岡田彩子,岡本公彰,加藤大明,宮之原真由,村田貴俊,山田秀則.
    • Total Pages
      42
    • Publisher
      医学情報社
    • ISBN
      9784903553719
    • Related Report
      2017 Annual Research Report

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Published: 2017-08-25   Modified: 2020-03-30  

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