Regulation mechanisms of lymphocyte trafficking by sphingosine 1-phosphate (S1P) transporters
| Project/Area Number |
17K08399
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Environmental and hygienic pharmacy
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| Research Institution | Setsunan University |
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Principal Investigator |
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| Project Period (FY) |
2017-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 赤血球 / S1P / 輸送体 / トランスポーター / 情報伝達物質 / S1P輸送体 / 蛍光測定 / CRISPR/Cas9 / MFSD2B / 脂質メディエーター |
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| Outline of Final Research Achievements |
Sphingosine 1-phosphate (S1P) is an intercellular lipid mediator essential for host defense against infection. Erythrocytes and endothelial cells release S1P into the blood stream. I analyzed gene-expression profiles of MEDEP-E14 cells that release S1P extracellularly and revealed that mfsd2b is the responsible gene for the S1P release activity. The charged residues D85 and K413 of MFSD2B are essential for the S1P transport in the cells. Expression of MFSD2B in mice erythrocytes strongly suggested that MFSD2B functions as an erythrocyte S1P transporter.
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| Academic Significance and Societal Importance of the Research Achievements |
赤血球のS1P輸送体MFSD2Bが見つかったことで、体内の主要なS1P輸送体がすべて明らかになった。S1Pは感染症から体を守る働きだけでなく、がん細胞の転移やAlzheimer 病にも関与する。MFSD2Bは赤血球だけでなく血小板のS1P輸送体としても働いており、今後MFSD2Bの未知の役割や薬の標的としての可能性が明らかになっていくと期待される。また、MFSD2Bとタンパク質の構造が非常に似ていても運ぶ物質の異なるMFSD2Aやタンパク質の構造がかなり異なっても運ぶ物質が同じSPNS2との比較により、MFSD2BのS1P輸送における詳細なメカニズムが解明できると考えている。
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Report
(5 results)
Research Products
(17 results)
