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Elucidation of the intracellular signal due to the repetition stretch stimulation to cells derived from keloid and hypertrophic scar

Research Project

Project/Area Number 17K17019
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Plastic surgery
Research InstitutionThe University of Tokushima

Principal Investigator

MINEDA Kazuhide  徳島大学, 大学院医歯薬学研究部(医学域), 助教 (70747815)

Research Collaborator SATO Katsuya  
Project Period (FY) 2017-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywordsケロイド / カルシウムシグナル応答 / ストレッチ刺激 / カルシウムイオン / 病的応答 / 線維芽細胞 / 伸展刺激 / カルシウムシグナル / 力学的刺激
Outline of Final Research Achievements

For fibroblasts derived from keloid (K-FBs) and fibroblasts derived from normal skin(N-FBs) , we gave stretching stimulation repeatedly and observed intracellular calcium ion concentration under the confocal microscope in real time.The cellular response rate of K-FBs groups was significantly high in comparison with the N-FBs groups (approximately 7%), and K-FBs groups (approximately 20%) showed high sensitivity for the stretching stimulation.The intracellular calcium ion concentration had the adjustment by negative feedback after the peak.When K-FBs was significantly high and supposed N-FBs to compare the peak value with the normal response, the cutoff value by the ROC analysis was 1.77, and it was speculated that the cell population indicating the pathologic response was present in approximately 13% in K-FBs.

Academic Significance and Societal Importance of the Research Achievements

本研究課題で、ケロイド由来線維芽細胞は繰り返しストレッチ刺激に易感受性であり、約13%に細胞内カルシウムイオン濃度の過剰な上昇を伴う病的応答が確認された。我々は、この病的応答を示す細胞集団がケロイド体質特有のものであると推察しており、原因として、小胞体ー細胞質間の細胞内カルシウムイオン濃度調節機構の機能異常でないかと考えている。今後、細胞内カルシウムイオン濃度を調節している小胞体膜上のカルシウムポンプ/チャネルについて検証し、ケロイド体質の解明を目指す。

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • Research Products

    (4 results)

All 2018 2017

All Presentation (4 results)

  • [Presentation] ケロイド由来線維芽細胞における繰り返しストレッチ刺激に起因したカルシウムシグナル応答の検討2018

    • Author(s)
      峯田一秀
    • Organizer
      第27回日本形成外科基礎学術集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 持続的な繰り返しストレッチ刺激に対する真皮線維芽細胞のCa2+シグナル応答2018

    • Author(s)
      今川尊稔、峯田一秀
    • Organizer
      第29回バイオフロンティア講演会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 前胸部ストレッチを模擬した刺激に対する正常およびケロイド線維芽細胞の刺激感受性の違い2018

    • Author(s)
      須谷和弘、峯田一秀
    • Organizer
      第29回バイオフロンティア講演会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 繰り返しストレッチ刺激を契機としたヒト線維芽細胞におけるカルシウムシグナル応答の検討2017

    • Author(s)
      峯田一秀
    • Organizer
      日本創傷治癒学会
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2020-03-30  

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