Effects of Metal Nanoparticles on Gut Microbiome and Immune System using Medaka

• Project/Area Number: 17K20047
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Research Field: Environmental analyses and evaluation and related fields
• Research Institution: Toyo University
• Principal Investigator: Shosaku Kashiwada 東洋大学, 生命科学部, 教授 (20370265)
• Co-Investigator(Kenkyū-buntansha): 宮西 伸光 東洋大学, 食環境科学部, 教授 (80372720)
• Project Period (FY): 2017-06-30 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2019: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2018: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: ナノ毒性 / 銀ナノ粒子 / 二酸化チタン / メダカ / 免疫毒性 / 腸内細菌叢 / 細菌叢 / 酸化毒性 / 免疫 / ナノ材料

Outline of Final Research Achievements

The effects of silver nanoparticles (SNC) and titanium dioxide nanoparticles (TiO2-NP) on the innate immunity and intestinal flora of medaka were evaluated by comprehensive genetic analysis and pathogen infection experiments. The bacterial flora associated with short-chain fatty acid production in the skin and intestine was found to be affected by SNC or TiO2-NP exposure. Investigation of the medaka with the infectious bacterium Edwardsiella piscicida showed that SNC exposure significantly reduced medaka survival, but TiO2-NP exposure did not. At least, SNC exposure was found to reduce intestinal short-chain fatty acids.

Academic Significance and Societal Importance of the Research Achievements

従来の化学物質の生態リスク研究は個体に対する直接的影響に関する内容であった。しかし生物は，一個体と雖も共生細菌叢（ミクロ生態系）との調和の下にその健全性が維持されている。本研究は，金属ナノ粒子をプローブとして，メダカと腸内ミクロ生態系が係るsynbioticsのみならず，免疫機能に対する影響の一端を生態毒性学的に明らかにした。メダカのsynbioticsはヒトにおけるそれとは異なると予測されたが、モデル生物メダカを用いた本研究成果は、今後の関連研究に対して貴重な情報の提供を行うだけでなく、脊椎生物モデルとしての利用価値を高めた。

Research Products

• [Int'l Joint Research] Ifremer(フランス)
• [Int'l Joint Research] Akvaplan Niva(ノルウェー)
• [Int'l Joint Research] Ifremer(フランス)
• [Presentation] 魚類毒性研究のための金属ナノ粒子のSP-ICP-MS分析手法の開発 (2019)
  • Author(s): 武島綾香，片岡知里，柏田祥策
  • Organizer: 第25回日本環境毒性学会研究発表会
• [Presentation] Immuno-toxic effects of silver nanocolloids and titanium dioxide nanoparticles on medaka fish (2018)
  • Author(s): Yumie Kato, Chisato Kataoka, Tadashi Ariyoshi, Kaori Shimizu, Hisato Takeuchi, Yoshihiro Kagami, Risa Horiuchi, Nobumitsu Miyanishi, Shosaku Kashiwada
  • Organizer: 31st Congress of New European Society for Comparative Physiology and Biochemistry
  • Int'l Joint Research
• [Presentation] Comparative toxicity of silver nanocolloids and titanium dioxide nanoparticles using medaka (2018)
  • Author(s): Yumie Kato, Chisato Kataoka, Tadashi Ariyoshi, Yoshihiro kagami, Shosaku Kashiwada
  • Organizer: Society of Environmental Toxicology and Chemistry Europe 28th Annual Meeting
  • Int'l Joint Research
• [Presentation] Environmental Risk of Silver Nanocolloids and Titanium Dioxide Nanoparticles on Immune Function and Pathogenic Tolerance of Medaka (2018)
  • Author(s): Yumie Kato, Chisato Kataoka, Tadashi Ariyoshi, Kaori Shimizu, Hisato Takeuchi, Yoshihiro Kagami, Risa Horiuchi, Nobumitsu Miyanishi and Shosaku Kashiwada
  • Organizer: 第21回環境ホルモン学会研究発表会
• [Presentation] Comparative toxicity of silver nanocolloids and titanium dioxide nanoparticles using medaka (2018)
  • Author(s): Yumie Kato, Tadashi Ariyoshi, Chisato Kataoka, Shosaku Kashiwada
  • Organizer: SETAC EU
  • Int'l Joint Research
• [Remarks] 東洋大学生命環境科学研究センター
  • URL: http://www.aqua-env.org/